Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3

Asimakoula, Stamatia; Marinakos, Orfeas; Tsagogiannis, Epameinondas; Koukkou, Anna‐Irini

doi:10.3390/microorganisms11020524

Cited by 16 publications

(5 citation statements)

References 54 publications

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“…Asphe3_35170 presents over 91% similarity with other Arthrobacter CDO12s, while Asphe3_40510 presents lower identity with other CDO23s. The function of Asphe3_38850/Asphe3_38860 (PCD34), Asphe3_42380 (PCD45) and Asphe3_35170 (CDO12) has been furtherly confirmed earlier by our research group [11,25,26].…”

Section: In Silico Analysis Of P Phenanthrenivorans Genome: 4-hba Deg...supporting

confidence: 78%

“…Utilization of 4-HBA as the Sole Carbon and Energy Source P. phenanthrenivorans Sphe3 was originally isolated from a creosote polluted site with the ability to grow on phenanthrene as the sole source of carbon and energy [24]. Sphe3 is a rather catabolically versatile bacterial strain as it is able to grow in the presence of various aromatic compounds, as reported in our previous studies [11,[25][26][27]. In the present study, Sphe3 was found to be capable of utilizing 4-HBA as the sole source of carbon and energy.…”

Section: Resultsmentioning

confidence: 83%

“…[28], or catechol in Klebsiella pneumoniae [29], Klebsiella aerogenes [30], Clostridium hydroxybenzoicum [31] and Enterobacter cloacae [32]. Catechol can then undergo the oxygenolytic cleavage of the aromatic ring via meta-fission (cat-alyzed by catechol 2,3-dioxygenase, CDO23) or ortho-fission (catalyzed by catechol 1,2dioxygenase, CDO12) to form 2-hydroxymuconic semialdehyde or cis,cis-muconic acid, respectively [25,33].…”

Section: In Silico Analysis Of P Phenanthrenivorans Genome: 4-hba Deg...mentioning

confidence: 99%

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Elucidation of 4-Hydroxybenzoic Acid Catabolic Pathways in Pseudarthrobacter phenanthrenivorans Sphe3

Tsagogiannis,

Asimakoula,

Drainas

et al. 2024

IJMS

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4-hydroxybenzoic acid (4-HBA) is an aromatic compound with high chemical stability, being extensively used in food, pharmaceutical and cosmetic industries and therefore widely distributed in various environments. Bioremediation constitutes the most sustainable approach for the removal of 4-hydroxybenzoate and its derivatives (parabens) from polluted environments. Pseudarthrobacter phenanthrenivorans Sphe3, a strain capable of degrading several aromatic compounds, is able to grow on 4-HBA as the sole carbon and energy source. Here, an attempt is made to clarify the catabolic pathways that are involved in the biodegradation of 4-hydroxybenzoate by Sphe3, applying a metabolomic and transcriptomic analysis of cells grown on 4-HBA. It seems that in Sphe3, 4-hydroxybenzoate is hydroxylated to form protocatechuate, which subsequently is either cleaved in ortho- and/or meta-positions or decarboxylated to form catechol. Protocatechuate and catechol are funneled into the TCA cycle following either the β-ketoadipate or protocatechuate meta-cleavage branches. Our results also suggest the involvement of the oxidative decarboxylation of the protocatechuate peripheral pathway to form hydroxyquinol. As a conclusion, P. phenanthrenivorans Sphe3 seems to be a rather versatile strain considering the 4-hydroxybenzoate biodegradation, as it has the advantage to carry it out effectively following different catabolic pathways concurrently.

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Section: In Silico Analysis Of P Phenanthrenivorans Genome: 4-hba Deg...supporting

confidence: 78%

Section: Resultsmentioning

confidence: 83%

Section: In Silico Analysis Of P Phenanthrenivorans Genome: 4-hba Deg...mentioning

confidence: 99%

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Elucidation of 4-Hydroxybenzoic Acid Catabolic Pathways in Pseudarthrobacter phenanthrenivorans Sphe3

Tsagogiannis,

Asimakoula,

Drainas

et al. 2024

IJMS

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“…Various microbial species are more adaptable and capable of rehabilitation in contaminated environments when exposed to phenol [ 36 , 37 ]. Bacteria have gained popularity as an eco-friendly and low-cost method of dealing with environmental pollutants [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Bacillus aryabhattai B8W22 peroxidase for phenol removal in waste water effluents

Elmetwalli¹,

Allam

Hassan

et al. 2023

BMC Microbiol

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Environmental contamination by phenol has been reported in both aquatic and atmospheric environments. This study aimed to separate and purify the peroxidase enzyme from bacteria that degrade phenol from wastewater sources. An enrichment culture of MSM was used to screen 25 bacterial isolates from different water samples for peroxidase production, six of the isolates exhibited high levels of peroxidase enzyme activity. Qualitative analysis of peroxidase revealed that isolate No. 4 had the highest halo zones (Poly-R478: 14.79 ± 0.78 mm, Azure B: 8.81 ± 0.61 mm). The promising isolate was identified as Bacillus aryabhattai B8W22 by 16S rRNA gene sequencing with accession number OP458197. As carbon and nitrogen sources, mannitol and sodium nitrate were utilized to achieve maximum peroxidase production. A 30-h incubation period was used with pH 6.0, 30 °C, mannitol, and sodium nitrate, respectively, for maximal production of peroxidase. Purified peroxidase enzyme showed 0.012 U/mg specific activity, and SDS-PAGE analysis indicated a molecular weight of 66 kDa. The purified enzyme exhibits maximum activity and thermal stability at pH values of 4.0 and 8.0, respectively, with maximum activity at 30 °C and complete thermal stability at 40 °C. In the purified enzyme, the Km value was 6.942 mg/ml and the Vmax value was 4.132 mol/ml/hr, respectively. The results demonstrated that Bacillus aryabhattai B8W22 has promising potential for degrading phenols from various phenol-polluted wastewater sources.

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“…Immobilization protects bacterial cells from the negative effects of toxic compounds and increases the concentration of toxicants for biodegradation. Thus, alginate-immobilized Pseudarthrobacter phenanthrenivorans Sphe3 cells decomposed phenol at a concentration of up to 1500 mg L −1 [ 22 ]. Chitosan-immobilized bacterial cells Chitinophaga spp., Methyloversatilis spp., Terrimonas spp.…”

Section: Introductionmentioning

confidence: 99%

Removal of Phenol by Rhodococcus opacus 1CP after Dormancy: Insight into Enzymes’ Induction, Specificity, and Cells Viability

Egozarian,

Emelyanova,

Suzina

et al. 2024

Microorganisms

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Biodegradation of phenol is an effective method for removing this toxicant from contaminated sites. Phenol is a toxic compound for living cells, so many bacteria degrade phenol in relatively low concentrations, up to 0.75 g L−1. The Rhodococcus opacus strain 1CP is an effective destructor of a wide range of pollutants. In the absence of a carbon source in the medium, cells of the R. opacus 1CP strain easily form cyst-like resting cells (CLC). The purpose of this work was to evaluate the viability of cells during long-term storage and the efficiency of the process of phenol destruction by R. opacus 1CP cells germinating after dormancy. Resting cells were obtained by simple cultivation in a rich medium followed by storage under static conditions. This is a simple approach to obtain a large amount of biomass. Decomposition of phenol proceeded via catechol followed by ortho-cleavage of aromatic ring. The induction of three phenol hydroxylases was detected by RT-PCR in cells germinated in a mineral medium with phenol as the carbon source. The stability of the genome of cells germinating after dormancy is shown by box-PCR. Dormant R. opacus 1CP cells, both suspended and immobilized, can be directly used for the decomposition of phenol after 4–12 months storage. In addition to phenol, after 9 months of storage, immobilized germinating cells easily metabolized 4-chlorophenol and 2,4,6-trichlorophenol. The results demonstrate a potential and simple approach toward achieving long-term storage of cells for further use in bioremediation.

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Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3

Cited by 16 publications

References 54 publications

Elucidation of 4-Hydroxybenzoic Acid Catabolic Pathways in Pseudarthrobacter phenanthrenivorans Sphe3

Elucidation of 4-Hydroxybenzoic Acid Catabolic Pathways in Pseudarthrobacter phenanthrenivorans Sphe3

Evaluation of Bacillus aryabhattai B8W22 peroxidase for phenol removal in waste water effluents

Removal of Phenol by Rhodococcus opacus 1CP after Dormancy: Insight into Enzymes’ Induction, Specificity, and Cells Viability

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