Monitoring the fate of small silver nanoparticles during artificial digestion

Kästner, Claudia; Lichtenstein, Dajana; Lampen, Alfonso; Thünemann, Andreas F.

doi:10.1016/j.colsurfa.2016.08.013

Cited by 39 publications

(67 citation statements)

References 18 publications

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“…Artificial in vitro digestion was originally based on DIN ISO 19738 and distinctly modified for scientific investigations on metals, metallic nanoparticles [19][20][21][22][23][24] , and other nanoparticles and biopolymers [25][26] . As described in Figure 1, the artificial in vitro digestion consists of three steps with the descried composition.…”

Section: Artificial In Vitro Digestionmentioning

confidence: 99%

Impact of an Artificial Digestion Procedure on Aluminum-Containing Nanomaterials

et al. 2017

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Aluminum has gathered toxicological attention based on relevant human exposure and its suspected hazardous potential. Nanoparticles from food supplements or food contact materials may reach the human gastrointestinal tract. Here, we monitored the physicochemical fate of aluminum-containing nanoparticles and aluminum ions when passaging an in vitro model of the human gastrointestinal tract. Small-angle X-ray scattering (SAXS), transmission electron microscopy (TEM), ion beam microscopy (IBM), secondary ion beam mass spectrometry (TOF-SIMS), and inductively coupled plasma mass spectrometry (ICP-MS) in the single-particle mode were employed to characterize two aluminum-containing nanomaterials with different particle core materials (Al, γAlO) and soluble AlCl. Particle size and shape remained unchanged in saliva, whereas strong agglomeration of both aluminum nanoparticle species was observed at low pH in gastric fluid together with an increased ion release. The levels of free aluminum ions decreased in intestinal fluid and the particles deagglomerated, thus liberating primary particles again. Dissolution of nanoparticles was limited and substantial changes of their shape and size were not detected. The amounts of particle-associated phosphorus, chlorine, potassium, and calcium increased in intestinal fluid, as compared to nanoparticles in standard dispersion. Interestingly, nanoparticles were found in the intestinal fluid after addition of ionic aluminum. We provide a comprehensive characterization of the fate of aluminum nanoparticles in simulated gastrointestinal fluids, demonstrating that orally ingested nanoparticles probably reach the intestinal epithelium. The balance between dissolution and de novo complex formation should be considered when evaluating nanotoxicological experiments.

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Section: Artificial In Vitro Digestionmentioning

confidence: 99%

Impact of an Artificial Digestion Procedure on Aluminum-Containing Nanomaterials

et al. 2017

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“…[34] These experiments do not consider their interaction with food components and the potential alterations occurring in the gastrointestinal tract, [35][36][37] while it has been shown that AgNPs can be affected by the surrounding conditions. [38] Due to their high reactivity, NMs might interact with food components, such as carbohydrates, lipids, proteins, and minerals, which may influence their size, charge, agglomeration state, and surface composition. [35,37,39,40] Besides, once ingested, AgNPs pass through the GIT in which physiological parameters (e.g., pH, ionic strength, protein composition) are constantly evolving and thus influencing the features of AgNPs throughout the digestion process.…”

Section: Introductionmentioning

confidence: 99%

“…[35,37,39,40] Besides, once ingested, AgNPs pass through the GIT in which physiological parameters (e.g., pH, ionic strength, protein composition) are constantly evolving and thus influencing the features of AgNPs throughout the digestion process. [38,41,42] As toxic effects of NMs are related to their physicochemical properties, [21,43] all the alterations that could take place before AgNPs attain the intestinal cells might affect their potential toxicity [35,39] and must therefore be included in cytotoxicity investigations to realize an accurate risk assessment. [37] The European Food Safety Authority (EFSA) has published a guidance in which recommendations were given for risk assessment of nanomaterials used in the food chain.…”

Section: Introductionmentioning

confidence: 99%

The Food Matrix and the Gastrointestinal Fluids Alter the Features of Silver Nanoparticles

Laloux

Kastrati

Cambier

et al. 2020

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Silver nanoparticles (AgNPs) are used in the agri‐food sector, which can lead to their ingestion. Their interaction with food and their passage through the gastrointestinal tract can alter their properties and influence their fate upon ingestion. Therefore, this study aims at developing an in vitro method to follow the fate of AgNPs in the gastrointestinal tract. After incorporation of AgNPs into a standardized food matrix, a precolonic digestion is simulated and AgNPs are characterized by different techniques. The presence of food influences the AgNPs properties by forming a corona around nanoparticles. Even if the salivary step does not impact significantly the AgNPs, the pH decrease and the digestive enzymes induce the agglomeration of AgNPs during the gastric phase, while the addition of intestinal fluids disintegrates these clusters. AgNPs can thus reach the intestinal cells under nanometric form, although the presence of food and gastrointestinal fluids modifies their properties compared to pristine AgNPs. They can form a corona around the nanoparticles and act as colloidal stabilizer, which can impact the interaction of AgNPs with intestinal epithelium. This study demonstrates the importance of taking the fate of AgNPs in the gastrointestinal tract into account to perform an accurate risk assessment of nanomaterials.

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“…Zusätzlich unterstützen oberflächenaktive Stoffe wie Gallensäuren, Phospholipide, Fettsäuren und Proteine die Zersetzung der Nahrung und, abhängig von der jeweiligen Materialbeschaffenheit, potenziell auch die von nanoskaligen Strukturen. Je nach individueller physikochemischer Natur des betrachteten Nanopartikels kann infolge der chemischen Umgebung des Magen-Darm-Trakts unterUmständen eine vollständige Auflösung anorganischer Nanopartikel oder eine Aggregation zu größeren Partikeln erfolgen [27][28][29]. Umgekehrt konnte gleichzeitig eine Neubildung von nanoskaligen Strukturen aus gelösten Verbindungen im Magen-Darm-Trakt gezeigt werden [30].…”

Section: Introductionunclassified

“…Stattdessen kommen zum Studium dieser Phänomene zumeist artifizielle Modelle des Verdauungstrakts zur Anwendung, welche die verschiedenen Abschnitte der gastrointestinalen Passage und ihre chemischen Gegebenheiten, wie etwa den Speichel in der Mundhöhle, den Magensaft oder die intestinale Flüs-sigkeit, nachstellen. Dies ermöglicht eine Untersuchung der Partikeleigenschaften entlang der Teilschritte der Verdauung auch unter der Berücksichtigung der gleichzeitigen Präsenz verschiedener Nahrungsmittelbestandteile [27,28,30,32,33]. In diesem Zusammenhang wird diskutiert und untersucht, inwieweit Nanopartikel aufgrund ihrer geringen Grö-ße möglicherweise bevorzugt physiologische Barrieren überwinden und somit in Zellen oder Gewebe aufgenommen werden können.…”

Section: Introductionunclassified

Nanomaterialien in Lebensmitteln – toxikologische Eigenschaften und Risikobewertung

Böhmert

Laux

Luch

et al. 2017

Bundesgesundheitsbl

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Monitoring the fate of small silver nanoparticles during artificial digestion

Cited by 39 publications

References 18 publications

Impact of an Artificial Digestion Procedure on Aluminum-Containing Nanomaterials

Impact of an Artificial Digestion Procedure on Aluminum-Containing Nanomaterials

The Food Matrix and the Gastrointestinal Fluids Alter the Features of Silver Nanoparticles

Nanomaterialien in Lebensmitteln – toxikologische Eigenschaften und Risikobewertung

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