Monitoring of tumour progression using bioluminescence imaging and computed tomography scanning in a nude mouse orthotopic model of human small cell lung cancer

The Biology of Malignant Mesothelioma and the Relevance of Preclinical Models

“…However, data have shown that magnetic resonance imaging (MRI) is a more reliable method for MPM tumor burden measurement compared to bioluminescence (57). Computed tomography scanning may be also of interest, as shown with a lung cancer cell line in mice (58). Tumor lesions and the localization of In the following subsections, we detail the present and ongoing models, with a focus on their interest, limitations, and impacts to assess emerging therapies.…”

Beneficial role of overexpression of TFPI‐2 on tumour progression in human small cell lung cancer

“…Moreover, genome sequencing of NCI‐H209 cells recently identified the combined loss of RB1 and TP53 typically observed in SCLC, demonstrating that this cell line clearly represents this disease [41,42]. Using this cell line, that we previously genetically modified to express firefly luciferase, we developed a reproducible and reliable nude mouse orthotopic model that resembles human SCLC [34]. The growth of the primary tumour was sensitively and non‐invasively followed by bioluminescence imaging that allowed real‐time monitoring of tumour progression in the same animals over a 2‐ to 9‐week period without sacrificing animals at different tumour stages.…”

“…Floating aggregate cells were grown in RPMI-1640 medium (Invitrogen, Cergy Pontoise, France) supplemented with 2 mM l -glutamine, 100 IU / mL penicillin, 100 μg / mL streptomycin and 10% endotoxin-free heat-inactivated foetal calf serum (FCS) (Lonza, Basel, Switzerland) at 37 • C in a humidified atmosphere with 5% CO 2 . Cells were then transfected with 4 μg of pCMV-luc plasmid (Clontech, Saint Quentin en Yvelines, France) using 10 μg Lipofectamine 2000 reagent according to the manufacturer's instructions (Invitrogen) and as previously described [ 34 ]. After transfection, cells were selected in complete medium containing 300 μg / mL G418, and stable clones expressing the highest levels of luciferase (NCI-H209 Luc cells) were isolated and cultured with 50 μg / mL G418 (Invitrogen).…”

“…Before implantation, NCI-H209 Luc cells were collected after centrifugation (3 min, 200 g ) and washed twice in FCS-free medium, and the Trypan Blue dye exclusion test was used to assess cell viability > 95%. The intrabronchial tumour cell implantation procedure was performed as previously described [ 34 ]. Mice were anaesthetised as previously described and the surgery area prepared with skin disinfection using betadine swabs.…”

New Researches and Application Progress of Commonly Used Optical Molecular Imaging Technology

“…The whole progression of disease can be monitored in combination with reporter gene. Currently, it has been used for monitoring tumor growth and metastasis, infection progress of bacteria or virus, transplantation, transgenic expression, and gene therapy [31]. …”