New Researches and Application Progress of Commonly Used Optical Molecular Imaging Technology

Chen, Zhiyi; Wang, Yixiang; Yang, Feng; Lin, Ye; Zhou, Qiulan; Liao, Yangying

doi:10.1155/2014/429198

Cited by 7 publications

(7 citation statements)

References 106 publications

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“…Для отслеживания клеток в организме реципиента используемые методы не должны нарушать жизнеспособность клеток и влиять на их способность к делению [9]. Предлагаемые на сегодняшний день способы по визуализации вводимых клеточных структур включают в себя неоптические, оптические и гибридные методы [10,11]. Однако большинство из них продемонстрировано лишь на уровне экспериментальных моделей [12].…”

Section: Discussionunclassified

Autologous mesenchymal stem cells in treatment of liver cirrhosis: evaluation of effectiveness and visualization method

Kotkas

Енукашвили

Asadulayev

et al. 2020

Science and Innovations in Medicine

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Through clinical observation, we present an assessment of the autologous mesenchymal stem cells effectiveness in treatment of liver cirrhosis of alimentary etiology. In order to determine the localization of the implanted cell structures, the stem cells were previously labeled with iron (II, III) oxide nanoparticles (IONPs). Further MRI visualization helped to detect the cell structures stained with iron oxide nanoparticles in the human body. In 6 months after the cell therapy, the patient underwent clinical and biochemical blood tests, MEGX test, elastography and subjective health assessment test. The tests data analysis revealed the improvement of the values of all examined parameters after the cell treatment. Also in 6 and 12 months after the treatment, a liver biopsy was performed from the area where the implanted stem cells were visualized. In histological examination of liver bioptates obtained from the area of MSC transplantation, the largest number of stained cells was observed in liver micronodes, as well as at the boundaries of micronodes and fibrous septa. A portion of the bioptate obtained in 12 months after transplantation was used to produce primary cell cultures. Before the first re-seeding of the cultures, cell colonies of both fibroblast-like morphology and epithelial were detected in them. Both types of colonies contained the particles. Conducting the cell therapy to a patient with liver cirrhosis of alimentary etiology contributed to improving the laboratory and instrumental examinations indicators. The patient had come through the treatment procedure satisfactorily, no complications were registered.

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Section: Discussionunclassified

Autologous mesenchymal stem cells in treatment of liver cirrhosis: evaluation of effectiveness and visualization method

Kotkas

Енукашвили

Asadulayev

et al. 2020

Science and Innovations in Medicine

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“…Fluorescence imaging, only used for short-term effect of drugs on the viability of the protoscolices, was restricted by the degenerative trend of fluorescence inevitably. Therefore, we also exerted effort to achieve a persistent bioluminescence model characterized by non-invasiveness, high sensitivity, and simple operation in comparison with conventional imaging modalities [ 16 ]. However, while most of the models that have been previously reported mainly concentrate on monocytes, such as stem and tumor cells [ 27 , 28 ], the plasmids we constructed failed to transfect protoscolices and achieve luciferase expression.…”

Section: Discussionmentioning

confidence: 99%

“…Optical molecular imaging (OMI) is a rapidly developing biomedical research discipline that allows the visual representation, characterization, and quantification of biological processes at the microscopic and macroscopic level within intact living organisms; the technique was developed based on genomics, proteomics, and modern optical imaging techniques through qualitative or quantitative observations and research of the activities of molecules and cells in physiological and pathological processes in vivo using specific molecular markers (e.g., luciferase and fluorescent protein) [ 14 , 15 ]. OMI is increasingly being utilized in disease diagnosis and therapeutic effect evaluation, and some research achievements have been obtained in preclinical studies [ 16 ]. The technique has gained popularity over the past decade because of its easy translation from in vitro to in vivo experiments, high sensitivity, and applicability to the fields of cancer, stem cells, and infectious diseases [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Construction of In Vivo Fluorescent Imaging of <i>Echinococcus granulosus</i> in a Mouse Model

et al. 2016

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Human hydatid disease (cystic echinococcosis, CE) is a chronic parasitic infection caused by the larval stage of the cestode Echinococcus granulosus. As the disease mainly affects the liver, approximately 70% of all identified CE cases are detected in this organ. Optical molecular imaging (OMI), a noninvasive imaging technique, has never been used in vivo with the specific molecular markers of CE. Thus, we aimed to construct an in vivo fluorescent imaging mouse model of CE to locate and quantify the presence of the parasites within the liver noninvasively. Drug-treated protoscolices were monitored after marking by JC-1 dye in in vitro and in vivo studies. This work describes for the first time the successful construction of an in vivo model of E. granulosus in a small living experimental animal to achieve dynamic monitoring and observation of multiple time points of the infection course. Using this model, we quantified and analyzed labeled protoscolices based on the intensities of their red and green fluorescence. Interestingly, the ratio of red to green fluorescence intensity not only revealed the location of protoscolices but also determined the viability of the parasites in vivo and in vivo tests. The noninvasive imaging model proposed in this work will be further studied for long-term detection and observation and may potentially be widely utilized in susceptibility testing and therapeutic effect evaluation.

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“…Therefore, the researchers combined the prior information of the structure into the FMT reconstruction, proposed a non-homogeneous imaging space model and a priori reconstruction method, which greatly improved the reconstruction accuracy. The structure of imaging space prior information can usually be obtained by high-resolution structural imaging modalities such as computed tomography (CT), magnetic resonance imaging (MRI) [91][92][93][94][95][96]. The optical parameters of various organs and tissues can be obtained by other imaging techniques such as diffuse optical tomography (DOT) [97].…”

Section: Inverse Problem Solvingmentioning

confidence: 99%

Recent methodology advances in fluorescence molecular tomography

Wang

Tian

2018

Vis. Comput. Ind. Biomed. Art

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Molecular imaging (MI) is a novel imaging discipline that has been continuously developed in recent years. It combines biochemistry, multimodal imaging, biomathematics, bioinformatics, cell & molecular physiology, biophysics, and pharmacology, and it provides a new technology platform for the early diagnosis and quantitative analysis of diseases, treatment monitoring and evaluation, and the development of comprehensive physiology. Fluorescence Molecular Tomography (FMT) is a type of optical imaging modality in MI that captures the three-dimensional distribution of fluorescence within a biological tissue generated by a specific molecule of fluorescent material within a biological tissue. Compared with other optical molecular imaging methods, FMT has the characteristics of high sensitivity, low cost, and safety and reliability. It has become the research frontier and research hotspot of optical molecular imaging technology. This paper took an overview of the recent methodology advances in FMT, mainly focused on the photon propagation model of FMT based on the radiative transfer equation (RTE), and the reconstruction problem solution consist of forward problem and inverse problem. We introduce the detailed technologies utilized in reconstruction of FMT. Finally, the challenges in FMT were discussed. This survey aims at summarizing current research hotspots in methodology of FMT, from which future research may benefit.

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New Researches and Application Progress of Commonly Used Optical Molecular Imaging Technology

Cited by 7 publications

References 106 publications

Autologous mesenchymal stem cells in treatment of liver cirrhosis: evaluation of effectiveness and visualization method

Autologous mesenchymal stem cells in treatment of liver cirrhosis: evaluation of effectiveness and visualization method

Construction of In Vivo Fluorescent Imaging of <i>Echinococcus granulosus</i> in a Mouse Model

Recent methodology advances in fluorescence molecular tomography

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