Monitoring of the heavy‐metal hyperaccumulation in vegetal tissues by X‐ray radiography and by femto‐second laser induced breakdown spectroscopy

Kaiser, Jozef; Samek, Ota; Reale, L.; Ka, Marsh; Malina, Radomír; Ritucci, A.; Poma, Anna; Tucci, Adele; Flora, F.; Lai, Antonia; Mancini, Lucia; Tromba, Giuliana; Zanini, Franco; Faenov, A. Ya.; Pikuz, T. A.; Cinque, Gianfelice

doi:10.1002/jemt.20394

Cited by 84 publications

(31 citation statements)

References 19 publications

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“…This process is very similar to that we could observe in the case of Cu accumulation in Dyplotaxis erucoides (Kaiser et al, 2005). Moreover, it seems that there is also a different intake process in the stem of Z. mays in comparison with the Helianthus annuus as it results from a dual-energy study previously conducted on this sunflower sample (Kaiser et al, 2007).…”

Section: Resultssupporting

confidence: 71%

“…A detailed account of the dual-energy algorithm and its implementation for elemental mapping were given in our earlier articles (Kaiser et al, 2005(Kaiser et al, , 2007Reale et al, 2008). In those experiments, dual-energy X-ray microradiography were tested on different plant samples grown in laboratory conditions with contaminants (e.g., Pb, Cd, Cu) added in controlled doses.…”

Section: The Dual-energy Algorithmmentioning

confidence: 99%

“…Moreover, synchrotron radiation can be tuned at the desired wavelength, and because of its high intensity, it is also able to detect very low amount of contaminants. Different techniques can be applied using X-rays from synchrotron sources: dual-energy (i.e., imaging of samples at X-ray energy above and below the edge of the heavy elements under study) radiography and tomography (Kaiser et al, 2005(Kaiser et al, , 2007Reale et al, 2008), projection microscopy with zone plate lenses (Yada 2009;Awaji et al, 2001), diffraction microscopy (Shapiro et al, 2005), X-ray scanning transmission microscopy (Hornberger et al, 2007), as well as X-ray microfluorescence.…”

Section: Introductionmentioning

confidence: 99%

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Detection of lead in Zea mays by dual‐energy X‐ray microtomography at the SYRMEP beamline of the ELETTRA synchrotron and by atomic absorption spectroscopy

Reale

Kaiser

Pace

et al. 2009

Microscopy Res & Technique

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This study is related to the application of the X-ray dual-energy microradiography technique together with the atomic absorption spectroscopy (AAS) for the detection of lead on Zea mays stem, ear, root, and leaf samples. To highlight the places with lead intake, the planar radiographs taken with monochromatic X-ray radiation in absorption regime with photon energy below and above the absorption edge of a given chemical element, respectively, are analyzed and processed. To recognize the biological structures involved in the intake, the dual-energy images with the lead signal have been compared with the optical images of the same Z. mays stem. The ear, stem, root, and leaf samples have also been analyzed with the AAS technique to measure the exact amount of the hyperaccumulated lead. The AAS measurement revealed that the highest intake occurred in the roots while the lowest in the maize ears and in the leaf. It seems there is a particular mechanism that protects the seeds and the leaves in the intake process.

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Section: Resultssupporting

confidence: 71%

Section: The Dual-energy Algorithmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of lead in Zea mays by dual‐energy X‐ray microtomography at the SYRMEP beamline of the ELETTRA synchrotron and by atomic absorption spectroscopy

Reale

Kaiser

Pace

et al. 2009

Microscopy Res & Technique

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show abstract

“…Likewise, the distribution of metal ions in the plant tissues and organs must be analysed because of different capability of different plant tissues to detoxify metal ions. The diagnostic techniques enabling three-dimensional monitoring of distribution of elements within different plant tissues and organs include mainly X-ray imaging methods [31 -33] [34]. Although the X-ray radiation based methods are widely used in laboratories, these methods have some disadvantages, especially limitation in utilization for in-situ analysis only.…”

Section: Introductionmentioning

confidence: 99%

Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

et al. 2010

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The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy.

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“…Kaiser et al [7] compared femtosecond LIBS with X-ray radiography for spatially resolving Cd and Pb in leaves and roots, although quantitative results were not obtained because standard reference samples were not obtained. Similarly, Samek et al [8] demonstrated the utility of femtosecond LIBS to image Fe accumulation in leaf veins and nearby leaf material with high spatial resolution and sensitivity.…”

Section: Figure 1 Laser-induced Breakdown Spectroscopy (Libs) Measurmentioning

confidence: 99%

Feedstock Quality Factor Calibration and Data Model Development

Boardman¹,

Westover²,

Gresham³

2010

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Objective:The goal of the feedstock assembly operation is to deliver uniform, quality-assured feedstock materials that will enhance downstream system performance by avoiding problems in the conversion equipment. In order to achieve this goal, there is a need for rapid screening tools and methodologies for assessing the thermochemical quality characteristics of biomass feedstock through the assembly process. Laser-induced breakdown spectroscopy (LIBS) has been identified as potential technique that could allow rapid elemental analyses of the inorganic content of biomass feedstocks; and consequently, would complement the carbohydrate data provided by near-infrared spectrometry (NIRS). These constituents, including Si, K, Ca, Na, S, P, Cl, Mg, Fe and Al, create a number of downstream problems in thermochemical processes. In particular, they reduce the energy content of the feedstock, influence reaction pathways, contribute to fouling and corrosion within systems, poison catalysts, and impact waste streams. Expected Outcome:The mineral content and composition is recognized as both a barrier to the use of some feedstocks and as a quality attribute that may be controlled through the feedstock assembly process. The inorganic content of biomass feedstocks is known to vary over wide ranges, depending upon the plant type and anatomical fraction, growing conditions, harvesting time and method, and handling operations. If this task is successful in developing a cost-effective screening tool, there may be opportunities for selecting and blending diverse feedstocks to produce on-spec materials that will in turn, reduce the capital and maintenance costs associated with syngas cleaning and replacing components in conversion platforms. Progress:Scoping studies have been conducted with the instrumentation in order to establish the feasibility of using the LIBS technique as a feedstock screening tool and to determine the optimal system operational parameters. These parameters include identifying the most sensitive, interference-free analytical lines for key elements and the laser ablation pulse parameters for sampling the materials. The sensitivity of the analytical lines to changes in elemental concentrations over ranges of interest has been benchmarked against independent analytical analyses conducted by Hazen Research Inc.

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Monitoring of the heavy‐metal hyperaccumulation in vegetal tissues by X‐ray radiography and by femto‐second laser induced breakdown spectroscopy

Cited by 84 publications

References 19 publications

Detection of lead in Zea mays by dual‐energy X‐ray microtomography at the SYRMEP beamline of the ELETTRA synchrotron and by atomic absorption spectroscopy

Detection of lead in Zea mays by dual‐energy X‐ray microtomography at the SYRMEP beamline of the ELETTRA synchrotron and by atomic absorption spectroscopy

Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

Feedstock Quality Factor Calibration and Data Model Development

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