Monitoring of the concentration of β-lactam antibiotics and their precursors in complex cultivation media by high-performance liquid chromatography

Schügerl, K.; Seidel, G.

doi:10.1016/s0021-9673(98)00264-7

Cited by 22 publications

(8 citation statements)

References 26 publications

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“…Schügerl and Seidel [9] study the analytical methods and special requirements for monitoring the production of the most important b-lactam antibiotics, penicillin G and V (PEN-G and V), produced by P. chrysogenum, and cephalosporin C produced by Acremonium chrysogenum, in complex cultivation media by HPLC.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Samanidou

Evaggelopoulou

Papadoyannis³

2006

J of Separation Science

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Natural penicillin (benzylpenicillin) is the oldest antibiotic observed by Alexander Fleming in 1928. To broaden its spectrum of activity, natural penicillin was modified, giving rise to a group of antibiotics under the name 'penicillins'. Although an increasing number of bacteria appear to be resistant to them, penicillins are used to treat a variety of bacterial infections including Gram-positive, Gram-negative aerobic and anaerobic bacteria. Consequently, they are widely used in human and veterinary medicine to prevent and treat diseases. This review covers the analytical methodologies, mainly chromatographic, employed to the penicillins determination in pharmaceutical formulations, biological fluids and in production-scale fermentations reported in the literature. Results of published assays are comparatively presented focusing on sample preparation regarding isolation and purification, chromatographic conditions and method validation. Information on chemical structure, spectrum of activity and action mechanism of common penicillins has also been given.

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Section: Introductionmentioning

confidence: 99%

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Samanidou

Evaggelopoulou

Papadoyannis³

2006

J of Separation Science

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“…The samples were then centrifuged at 8000 rpm for 15 min. The supernatants were collected and stored for CPC, DAOC and glucose measurements . After properly diluting the supernatants, glucose concentrations were measured by biosensor (SBA‐40B, Shangdong Science Academy, China).…”

Section: Methodsmentioning

confidence: 99%

Enhanced cephalosporin C production with a novel DO‐Stat based carbon resources co‐feeding strategy

Duan

Gao³

et al. 2013

J of Chemical Tech & Biotech

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Background To solve the problems of high by‐product accumulations and low substrate utilization efficiencies in the traditional cephalosporin C (CPC) fermentation process, a novel DO‐Stat based substrates co‐feeding strategy for CPC production by Acremonium chrysogenum (A. chrysogenum) was conducted in a 7 L fermentor using the addition of glucose coupled with soybean oil. RESULTS Compared with the sole soybean oil feeding method, the final CPC concentration was enhanced from 25.3 g L‐1 to a higher level of 31.9 g L‐1 by the novel substrate co‐feeding strategy. The major by‐product of deacetoxycephalosporin C (DAOC) was reduced from 0.32 g L‐1 to a lower level of 0.092 g L‐1, and the final DAOC/CPC ratio (0.28%) reached the CPC fermentation quality standard (below 0.5%). Metabolic flux analysis revealed that the carbon fluxes in the CPC synthesis related route were increased about 2.2‐fold by applying the novel substrate co‐feeding strategy. Conclusion The results indicated that the co‐feeding of glucose and soybean oil in the main CPC production phase was desirable for CPC fermentation, and led to higher CPC production/yield and substrate utilization efficiencies but less DAOC accumulation. © 2013 Society of Chemical Industry

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“…The full power of contemporary separation methods has rarely been implemented. There are several reports on using high performance liquid chromatography (HPLC) to monitor bacterial processes [7][8][9][10][11], but HPLC is an inherently slow method, might lack efficiency and definitely requires sophisticated sample preparation procedures in the case of bacterial process monitoring. The on-line HPLC monitoring of bioprocesses has been critically discussed in the literature [2][3][4][5][6][7][8][9][10][11][12][13] as it appears not to be appropriate for process control.…”

Section: Introductionmentioning

confidence: 99%

“…There are several reports on using high performance liquid chromatography (HPLC) to monitor bacterial processes [7][8][9][10][11], but HPLC is an inherently slow method, might lack efficiency and definitely requires sophisticated sample preparation procedures in the case of bacterial process monitoring. The on-line HPLC monitoring of bioprocesses has been critically discussed in the literature [2][3][4][5][6][7][8][9][10][11][12][13] as it appears not to be appropriate for process control. Yet it has been established in the biological and medical monitoring [14] and approaches have been made in the development of a selective HPLC based on-line monitoring in wastewaters [15,16] and bioreactors [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Fermentation reactor coupled with capillary electrophoresis for on-line bioprocess monitoring

Tahkoniemi

Helmja

Menert

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

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Monitoring of the concentration of β-lactam antibiotics and their precursors in complex cultivation media by high-performance liquid chromatography

Cited by 22 publications

References 26 publications

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Chromatographic analysis of penicillins in pharmaceutical formulations and biological fluids

Enhanced cephalosporin C production with a novel DO‐Stat based carbon resources co‐feeding strategy

Fermentation reactor coupled with capillary electrophoresis for on-line bioprocess monitoring

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