Monitoring of Target-Site Mutations Conferring Insecticide Resistance in Spodoptera frugiperda

Boaventura, Debora; Martin, Macarena; Pozzebon, Alberto; Mota-Sánchez, David; Nauen, Ralf

doi:10.3390/insects11080545

Cited by 64 publications

(96 citation statements)

References 68 publications

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“…2018; Boaventura et al . 2020c). Furthermore, genetic markers provide a powerful way to characterise changes in the presence and frequency of resistance through time, as has been demonstrated in various mosquito species in Africa (Verhaeghen et al .…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, Boaventura et al . (2020c) observed widespread global distributions of ace1 mutations in South America, Africa and South East Asia. With respect to pyrethroid resistance, Zhang et al .…”

Section: Discussionmentioning

confidence: 99%

“…2020; Boaventura et al . 2020c). Low‐frequency para sodium channel mutations, associated with pyrethroid resistance, have been observed in populations from Brazil (Carvalho et al .…”

Section: Australian Trends In Insecticide Resistancementioning

confidence: 99%

“…2013) and in Indonesia (Boaventura et al . 2020c). Similarly, a mutation in the RyR gene has been found at low frequencies in Brazil and likely plays a role in diamide resistance (Boaventura et al .…”

Section: Australian Trends In Insecticide Resistancementioning

confidence: 99%

“…2020; Boaventura et al . 2020c). Cumulative global knowledge of resistance mechanisms is helpful for developing hypotheses and expectations at more localised scales.…”

Section: Introductionmentioning

confidence: 99%

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Empowering Australian insecticide resistance research with genetic information: the road ahead

2020

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Insecticides are important for chemical control of arthropod pests in agricultural systems but select for resistance as an adaptive trait. Identifying the genetic mechanism(s) underpinning resistance can facilitate development of genetic markers, which can be used in monitoring programs. Moreover, understanding of genetic mechanisms in a broader population genetic context can be used to infer the origins of resistance, predict the dynamics of resistance evolution and evaluate the efficacy of different management strategies. Transitioning genetic information successfully into practical solutions requires overcoming two major hurdles. Firstly, genetic mechanisms must be identified to develop genetic markers. Secondly, routine use of genetic markers is required to build substantial spatio‐temporal data on the distribution and frequency of resistance alleles. In this study, we demonstrate large knowledge gaps on the genetic mechanisms of insecticide resistances in Australia using eight established arthropod pests important to the grains industry: Bemisia tabaci (silverleaf whitefly), Frankliniella occidentalis (western flower thrips), Halotydeus destructor (redlegged earth mite), Helicoverpa armigera (cotton bollworm), Myzus persicae (green peach aphid), Plutella xylostella (diamondback moth), Tetranychus urticae (two‐spotted spider mite) and Thrips tabaci (onion thrips). Many resistances have not been characterised at the genetic level in most pests, even for chemical MoA groups with a long history of use in Australia. Moreover, monitoring of resistance is spatio‐temporally patchy, which precludes examination of long‐term trends or predictive modelling. We suggest that leveraging cumulative global knowledge of resistances to develop a priori candidate genes, and incorporation of genomic approaches, can help overcome the hurdles of embracing genetic information in resistance management. We highlight the recently invasive Spodoptera frugiperda (fall armyworm) as a case study where genetic markers and genomic approaches should prove useful in rapidly assessing the risk of this species to the Australian grains industry and other agricultural commodities. The uptake of genetic information into management can only occur once its benefit to empower insecticide resistance research is fully realised. Ultimately, the road ahead requires amalgamation of multifaceted data (genes, environment and spatio‐temporal replication) to better understand and predict the dynamics of resistance evolution.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…2020; Boaventura et al . 2020c). Low‐frequency para sodium channel mutations, associated with pyrethroid resistance, have been observed in populations from Brazil (Carvalho et al .…”

Section: Australian Trends In Insecticide Resistancementioning

confidence: 99%

Section: Australian Trends In Insecticide Resistancementioning

confidence: 99%

“…2020; Boaventura et al . 2020c). Cumulative global knowledge of resistance mechanisms is helpful for developing hypotheses and expectations at more localised scales.…”

Section: Introductionmentioning

confidence: 99%

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Empowering Australian insecticide resistance research with genetic information: the road ahead

2020

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CRISPR/Cas9‐mediated mutagenesis of Sex lethal (Sxl) gene impacts fertility of the Fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)

Chikmagalur Nagaraja,

Karuppannasamy,

Ramasamy

et al. 2023

Arch Insect Biochem Physiol

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Identification of novel approaches for managing the global pest, the Fall armyworm, Spodoptera frugiperda, is the need of the hour, as it defies many management strategies including synthetic chemicals, Bt transgenics, and so on. Recently CRISPR/Cas9‐based genome editing opened up newer avenues to design novel pest management strategies such as precision‐guided sterile insect technique (pgSIT). In this regard, genes governing sex determination, egg reproduction, and spermatogenesis could be the prime targets for genome editing. This requires validation of the target genes, preferably by a nontransgenic DNA‐free editing, before the final application. One such important gene regulating sex determination in Drosophila is the Sex lethal (Sxl). However, the function of Sxl is not highly conserved in other insects and, in particular, we are beginning to comprehend its role in Lepidoptera with only one reference available in Spodoptera litura till date. In the present study, we have edited the sxl gene of S. frugiperda through the delivery of ribonucleoprotein complex (sgRNA + Cas9) at G0 stage embryo, targeting the conserved region of all the documented five splice variants. Results clearly showed that editing of sxl gene impacted the overall fecundity and hatching rate. Therefore, Sxl could be one of the target genes for developing pgSIT approach for the management of S. frugiperda.

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Development of a simple and accurate molecular tool for Spodoptera frugiperda species identification using LAMP

Kim

Nam

Kwon³

et al. 2021

Pest Management Science

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BACKGROUND: The fall armyworm, Spodoptera frugiperda is a native species of the Americas. First detected in western and central Africa in early 2016, it has become one of the most serious invasive lepidopteran pests in many African and Asian countries. S. frugiperda has spread very quickly; however, there are no molecular-based, simple and accurate diagnostic tools for identification of this species in the field. Methods to identify invasive S. frugiperda are urgently needed because farmers and agricultural managers have no prior experience with this pest. RESULTS: Based on mitochondrial genome sequence alignment, a S. frugiperda-specific sequence region was identified in the transfer RNA-coding region between NADH dehydrogenase, ND3, and ND5. Using this unique region, species-diagnostic primers were designed and applied in a loop-mediated isothermal amplification (LAMP) assay and a conventional polymerase chain reaction to identify field-collected samples of S. frugiperda. The optimal incubation conditions for the LAMP assay were 61°C for 90 min with four LAMP primers; an additional loop primer increased the amplification efficiency. A response was obtained for a wide range of DNA concentrations in the LAMP assay and the minimum detectable DNA concentration was 10 pg.CONCLUSIONS: We developed a new LAMP-based molecular diagnostic method that it is easy to use and accurate. The LAMP assay was used with a DNA-releasing technique for larval and adult samples, without a DNA extraction step, by incubating the tissue sample at 95°C for 5 min. This method can be applied in intensive field monitoring of S. frugiperda and its ecological studies.

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Monitoring of Target-Site Mutations Conferring Insecticide Resistance in Spodoptera frugiperda

Cited by 64 publications

References 68 publications

Empowering Australian insecticide resistance research with genetic information: the road ahead

Empowering Australian insecticide resistance research with genetic information: the road ahead

CRISPR/Cas9‐mediated mutagenesis of Sex lethal (Sxl) gene impacts fertility of the Fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)

Development of a simple and accurate molecular tool for Spodoptera frugiperda species identification using LAMP

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