Monitoring of Selected Genes in Campylobacter Jejuni and Campylobacter Coli Isolates from Domestic Animals

Abstract

In the present study, the presence of flaA, cadF, cdtB, and iam genes of Campylobacter sp. were analysed using PCR. Material for analyses comprised 100 Campylobacter sp. isolates obtained from healthy broiler chickens, fatteners, and calves, among which 84 isolates were ascribed to Campylobacter jejeuni and 16 to Campylobacter coli. All isolates (100%) had the cadF gene responsible for adhesion and the flaA gene determining the motility of the analysed bacteria. The frequency of occurrence of the cdtB gene responsible for the production of the cytolethal distending toxin (CDT) was determined to be high (98.6% in broiler chickens, 75% in fatteners, 62.5% in calves). In case of the iam gene, the highest frequency was recorded in Campylobacter sp. isolated from broiler chickens (84.7%), while in strains collected from fatteners and calves it was lower, amounting to 41.7% and 18.8%, respectively.

The prevalence of Campylobacter spp. and occurrence of virulence genes isolated from dogs

“…Biswas et al (2011) confirmed the occurrence of this gene in 100% of the examined C. jejuni strains derived from human clinical studies and cow faeces. Selwet and Galbas (2012a;2012b) observed that the cadF and flaA genes were found in 100% of C. coli and C. jejuni isolated from broilers, porkers, calves and piglets. All the strains of C. jejuni, C. coli C. upsaliensis under analysis carried the cdtB gene encoding the protein exhibiting toxic properties, i.e.…”

Monitoring of Virulence Genes, Drug-Resistance in Campylobacter coli Isolated from Golden Retrievers

“…According to the investigations by Andrzejewska et al (2011) and Selwet and Galbas (2012a;2012b) on C. coli occurrence in people, dogs, cats, and piglets, the presence of the flaA gene in all the examined isolates was also recorded (Selwet et al, 2015). The next factor affecting virulence is gene cadF, which is responsible for production of adhesines.…”

“…In the experiments, the cadF gene was found also in 100% of isolates from both study groups. Some researchers attribute a high importance of the cadF gene leading to campylobacteriosis in human (Selwet and Galbas, 2012a). Cytokines such as interleukin-8 (IL-8) are secreted by host cells in response to bacterial invasion, acting as early warning signs to the host immune system, and Campylobacter spp.…”

“…are able of producing many toxins, which can damage red blood cells. Many of these toxins/hemolysins are considered virulence factors because of their ability to increase the availability of iron to the pathogen throughout the process of infection via lyses of erythrocytes and subsequent release of heme from hemoglobin (Istivan et al, 2008;Selwet and Galbas, 2012a). Summing up, the prevalence of C. coli in dogs are low, suggesting that this population of dogs is unlikely to be a common source of C. coli infection for humans.…”

An Assessment of the Occurrence of Selected Virulence and Antibiotic Resistance Genes in Bacteria of the Genus Campylobacter Collected from Horses

“…Thermotolerant bacteria of the Campylobacter genus (mainly Campylobacter jejuni and Campylobacter coli) are part of the natural intestinal flora of birds and mammals. They are also commonly found in water and soil contaminated with animal faeces [1]. Contact with faeces or with a sick animal and failure to comply with basic rules of hygiene may cause campylobacteriosis in humans [2].…”