The prevalence of Campylobacter spp. and occurrence of virulence genes isolated from dogs

Selwet, Marek; Cłapa, Tomasz; Galbas, Mariola; Słomski, Ryszard; Porzucek, Filip

doi:10.33073/pjm-2015-011

Cited by 14 publications

(15 citation statements)

References 18 publications

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“…12.6%. This result stood in opposition to our earlier studies [ 1 , 20 ] and research conducted by other authors [ 5 , 21 ]. Chaban et al [ 22 ] analysed a group ( n = 70) of healthy dogs and found bacteria of the Campylobacter genus in 58% of the samples.…”

Section: Discussioncontrasting

confidence: 99%

The prevalence of virulence genes and multidrug resistance in thermophilic Campylobacter spp. isolated from dogs

Selwet

2019

Open Life Sciences

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The aim of the study was to determine the role of dogs as a potential reservoir of Campylobacter spp. At the next stage of the research the frequency of occurrence of selected virulence genes, i.e. cadF, flaA and iam as well as genes responsible for the formation the cytolethal distending toxin (CDT), i.e. cdtA, cdtB and cdtC was determined. The isolates obtained in the research were tested for their resistance to selected antibiotics: ciprofloxacin (CIP), enrofloxacin (EF), erythromycin (E) and tetracycline (TE). Campylobacter spp. was found in 63 (12.6%) out of a total number of 500 isolates. 61 (12.2%) isolates were identified as C. jejuni. The number of C. jejuni isolates found in the younger animals was smaller (p <0.05) than in the older ones. The frequency of occurrence of virulence genes and the genes responsible for the formation of CDT was significantly (p <0.05) higher in the older dogs. A comparison of the effect of antibiotics showed that the isolates obtained from both age groups exhibited low resistance to erythrosine (13.5% in the group aged under 1 year and 8.6% in the group aged over 1 year). Both groups exhibited the highest resistance to ciprofloxacin and enrofloxacin.

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Section: Discussioncontrasting

confidence: 99%

The prevalence of virulence genes and multidrug resistance in thermophilic Campylobacter spp. isolated from dogs

Selwet

2019

Open Life Sciences

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“…fetus recovered from water and milk samples. In the studies of Lluque et al [ 77 ], Wieczorek et al [ 78 ] and Selwet et al [ 79 ], the cadF gene was detected in Campylobacter species from a Peruvian pediatric cohort, from meat samples and from Campylobacter isolates isolated from dogs and these reports corroborate our finding. The presence of one or more virulence genes in the Campylobacter genome give rise to the incidence of human infection (Abu-Madi et al [ 80 ]).…”

Section: Discussionsupporting

confidence: 90%

Occurrence, Virulence and Antimicrobial Resistance-Associated Markers in Campylobacter Species Isolated from Retail Fresh Milk and Water Samples in Two District Municipalities in the Eastern Cape Province, South Africa

Igwaran

Okoh

2020

Antibiotics

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Campylobacter species are among the major bacteria implicated in human gastrointestinal infections and are majorly found in faeces of domestic animals, sewage discharges and agricultural runoff. These pathogens have been implicated in diseases outbreaks through consumption of contaminated milk and water in some parts of the globe and reports on this is very scanty in the Eastern Cape Province. Hence, this study evaluated the occurrence as well as virulence and antimicrobial-associated makers of Campylobacter species recovered from milk and water samples. A total of 56 water samples and 72 raw milk samples were collected and the samples were processed for enrichment in Bolton broth and incubated for 48 h in 10% CO2 at 42 °C under microaerobic condition. Thereafter, the enriched cultures were further processed and purified. After which, presumptive Campylobacter colonies were isolated and later confirmed by PCR using specific primers for the detection of the genus Campylobacter, target species and virulence associated genes. Antimicrobial resistance profiles of the isolates were determined by disk diffusion method against a panel of 12 antibiotics and relevant genotypic resistance genes were assessed by PCR assay. A total of 438 presumptive Campylobacter isolates were obtained; from which, 162 were identified as belonging to the genus Campylobacter of which 36.92% were obtained from water samples and 37.11% from milk samples. The 162 confirmed isolates were further delineated into four species, of which, 7.41%, 27.16% and 8.64% were identified as C. fetus, C. jejuni and C. coli respectively. Among the virulence genes screened for, the iam (32.88%) was most prevalent, followed by flgR (26.87%) gene and cdtB and cadF (5.71% each) genes. Of the 12 antibiotics tested, the highest phenotypic resistance displayed by Campylobacter isolates was against clindamycin (95.68%), while the lowest was observed against imipenem (21.47%). Other high phenotypic resistance displayed by the isolates were against erythromycin (95.06%), followed by ceftriaxone (93.21%), doxycycline (87.65%), azithromycin and ampicillin (87.04% each), tetracycline (83.33%), chloramphenicol (78.27%), ciprofloxacin (77.78%), levofloxacin (59.88%) and gentamicin (56.17%). Relevant resistance genes were assessed in the isolates that showed high phenotypic resistance, and the highest resistance gene harbored by the isolates was catII (95%) gene while VIM, KPC, Ges, bla-OXA-48-like, tetC, tetD, tetK, IMI and catI genes were not detected. The occurrence of this pathogen and the detection of virulence and antimicrobial resistance-associated genes in Campylobacter isolates recovered from milk/water samples position them a risk to human health.

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“…The other studies reported Campylobacter infection rates of 4.81-51.1%. 5,[22][23][24][25][26][27] The variation in the rate of infection may rely on the sample size, age, diet, and gastrointestinal status (diarrheal or healthy) of the studied dogs, different diagnosis methods, and geographical location, as well as the season of sampling. The most prevalent species of Campylobacter among the dogs were C. coli (38.89%) and C. jejuni (33.33%).…”

Section: Discussionmentioning

confidence: 99%

“…Although there was no significant relationship between age and infection, several surveys have emphasized that dogs less than one year old were most likely to be infected with Campylobacter. 2,16,22,[24][25][26]32 It may be because of the weakness of the puppies and the low level of immunity in young dogs compared to adults. 22,30 Although Campylobacter was more prevalent in male compared to female dogs, the statistical analysis showed no significant difference, implying that Campylobacter exposure has no gender predilection in dogs, which is consistent with the results of a previous study.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Molecular Detection, and Risk Factors of Campylobacter Infection From Companion Dogs

Gharibi

Mosallanejad

Feyzabadi

2020

Int J Enteric Pathog

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Background: Campylobacter is an organism that is usually associated with diarrhea in pet animals and humans, as well as other domestic, wild, and laboratory animals. Objective: The aim of the present survey was the isolation, molecular detection, and risk factors of Campylobacter infection from companion dogs referred to the Veterinary Hospital of Ahvaz district, the South-West of Iran. Materials and Methods: Rectal swabs were examined by culture and polymerase chain reaction (PCR) methods from 122 companion dogs (52 diarrheic and 70 clinically healthy). Several risk factors were reviewed, including age, gender, breed, nutrition status, and lifestyle. Results: The results showed that only five samples (4.1%) were positive for Campylobacter spp. in the culture method. Campylobacter spp. was detected in 18 out of 122 dogs by the PCR, yielding an overall prevalence of 14.8%. The most prevalent species of Campylobacter among the referred dogs were C. coli (38.89%) and C. jejuni (33.33%). A lower prevalence was found for C. upsaliensis (11.11%) and C. lari (5.55%). Concurrent infections were observed in two cases of C. upsaliensis + C. lari (5.55%) and C. coli + C. lari (5.55%). No significant difference was noted between healthy (11.43%) and diarrheic (19.23%) dogs (P>0.05). Eventually, age, gender, breed, nutrition status, and lifestyle had no significant effect on Campylobacter infection (P>0.05). Conclusion: Although the prevalence of Campylobacter was moderate in the dog population of Ahvaz district, these bacteria can constitute a public health hazard because of the frequent presence of Campylobacter species in the feces.

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The prevalence of Campylobacter spp. and occurrence of virulence genes isolated from dogs

Cited by 14 publications

References 18 publications

The prevalence of virulence genes and multidrug resistance in thermophilic Campylobacter spp. isolated from dogs

The prevalence of virulence genes and multidrug resistance in thermophilic Campylobacter spp. isolated from dogs

Occurrence, Virulence and Antimicrobial Resistance-Associated Markers in Campylobacter Species Isolated from Retail Fresh Milk and Water Samples in Two District Municipalities in the Eastern Cape Province, South Africa

Isolation, Molecular Detection, and Risk Factors of Campylobacter Infection From Companion Dogs

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