Monitoring of radiotherapy-induced morphonuclear modifications in the mxt mouse mammary carcinoma by means of digital cell image analysis

Gasperin, Plínio; Gozy, M.; Pauwels, Olivier S. G.; Frohling, Janos; Houtte, Paul Van; Pasteels, Jean-Lambert; Kiss, Róbert

doi:10.1016/0360-3016(92)90797-l

Cited by 17 publications

(7 citation statements)

References 16 publications

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“…2). These radiosensitivity results correlated well with results from previous studies that had measured the response of several of these cell lines to X‐ray radiation (26–28).…”

Section: Resultssupporting

confidence: 87%

“…The value of chromatin phenotype in bladder cancer prognosis has also been confirmed by others (20, 21). Furthermore, nuclear texture analysis has also shown potential in assessing the relative sensitivity of bladder tumor cells to chemotherapeutic drugs (22, 23) and response to radiation (24–27). Taken together, these studies suggest that chromatin phenotype in bladder neoplasia, as measured using digital texture analysis, is a very strong biomarker.…”

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confidence: 99%

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Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype

et al. 2006

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Background: Nuclear texture analysis measures phenotypic changes in chromatin distribution within a cell nucleus, while the alkaline Comet assay is a sensitive method for measuring the extent of DNA breakage in individual cells. The authors aim to use both methods to provide information about the sensitivity of cells to ionizing radiation. Methods: The alkaline Comet assay was performed on six human bladder carcinoma cell lines and one human urothelial cell line exposed to γ‐radiation doses from 0 to 10 Gy. Nuclear chromatin texture analysis of 40 features was then performed in the same cell lines exposed to 0, 2, and 6 Gy to explore if nuclear phenotype was related to radiation sensitivity. Results: Comet assay results demonstrated that the cell lines exhibited different levels of radiosensitivity and could be divided into a radiosensitive and a radioresistant group at >6 Gy. Using stepwise discriminant analysis, a subset of important nuclear texture features that best discriminated between sensitive and resistant cell lines were identified A classification function, defined using these features, correctly classified 81.75% of all cells into their radiosensitive or radioresistant groups based on their pretreatment chromatin phenotype. Posttreatment chromatin changes also varied between cell lines, with sensitive cell lines showing a relaxed chromatin conformation following radiation, whereas resistant cell lines exhibited chromatin condensation. Conclusions: The authors conclude that the alkaline Comet assay and nuclear texture methodologies may prove to be valuable aids in predicting the response of tumor cells to radiotherapy. © 2006 International Society for Analytical Cytology

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“…2). These radiosensitivity results correlated well with results from previous studies that had measured the response of several of these cell lines to X‐ray radiation (26–28).…”

Section: Resultssupporting

confidence: 87%

mentioning

confidence: 99%

Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype

et al. 2006

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“…We have observer on the same basis as those previously described [11-16, previously tivity [14], and radiosensitivity [15].…”

Section: Introductionmentioning

confidence: 99%

“…the transformation of the absolute values of the DNA histogram abcissa into relative ones, where the 2C DNA content was 2,100 { 100 IOD relative units [11]. The SRL, LRL, and LM parameters were used the computer-assisted microscope analysis of computed on the basis of the run length and cooccurence matrices, respectively, as is detailed elsewhere [12][13][14][15][16]18]. …”

Section: Introductionmentioning

confidence: 99%

Hepatic Morphonuclear Alterations Following Portacaval Shunt in the Rat

Ravelingien

Kiss²,

Pector

1996

Journal of Surgical Research

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“…Neoplastic mouse mammary cells and human bladder cells that are resistant to chemotherapy possess features that appear to be linked to their resistant state rather than to their tissue of origin or to the drug used to induce resistance: the cells have smaller nuclei and lower optical densities than normal cells, and their chromatin displays pale areas and some hyperchromatic clumping (13). Nuclei of MXT cells subjected to radiotherapy are larger in area and con-tain less condensed chromatin than the nuclei of control MXT cells (25).…”

Section: Discussionmentioning

confidence: 79%

Characterization of factors in routine laboratory protocols that significantly influence the Feulgen reaction.

Kiss¹,

Salmon²,

Camby³

et al. 1993

J Histochem Cytochem.

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We investigated the parameters that could affect the cytophotometric analysis of cell nuclei stained by the Feulgen reaction. These parameters included: the hydrolysis temperature (in the normal "room temperature" range); the composition of the S C W s teagent; the speed of centrifugation of the cell suspensions; the mode of preservation [air-drying or ethanol-formalin-acetic acid (EM) fmtion]; the fmuon time; the pronase digestion time; and the concentration of pronase used to obtain cell suspensions from archival (formalin-fired, pataffin-embedded) materials. Relatively homogeneous material was studied: the MXT mouse mammary adenocarcinoma growing in vivo as tumors with both small and hyperchromatic cell nuclei and in vitro as monolayers with larger and less hyperchromatic cell nuclei. The results of these investigations demonstrate the necessity for the precise definition of a protocol for such procedures

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Monitoring of radiotherapy-induced morphonuclear modifications in the mxt mouse mammary carcinoma by means of digital cell image analysis

Cited by 17 publications

References 16 publications

Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype

Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype

Hepatic Morphonuclear Alterations Following Portacaval Shunt in the Rat

Characterization of factors in routine laboratory protocols that significantly influence the Feulgen reaction.

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