Characterization of factors in routine laboratory protocols that significantly influence the Feulgen reaction.

Kiss, Róbert; Salmon, Isabelle; Camby, Isabelle; Gras, Stéphane; Pasteels, Jean Lambert

doi:10.1177/41.6.8315284

Cited by 57 publications

(16 citation statements)

References 28 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Labeling of nuclear DNA by the Feulgen reaction 15 was developed in 1924 and was combined with cytophotometry in 1964. 16 Unfortunately, this protocol continues to be used, despite significant limitations, which include type and duration of fixation, 17 extent of DNA hydrolysis required for the Schiff reaction, 18,19 and the chromatin structure. The latter is critical for the accessibility of the DNA by this technique.…”

Section: Resultsmentioning

confidence: 99%

Cardiomyogenesis in the Aging and Failing Human Heart

et al. 2012

View full text Add to dashboard Cite

Background Two opposite views of cardiac growth are currently held: one views the heart as a static organ, characterized by a large number of cardiomyocytes, which are present at birth and live as long as the organism; and the other views the heart a highly plastic organ in which the myocyte compartment is restored several times during the course of life. Methods and Results The average age of cardiomyocytes, vascular endothelial cells (ECs), and fibroblasts and their turnover rates were measured by retrospective 14C birth dating of cells in 19 normal hearts, 2 to 78 years of age, and in 17 explanted failing hearts, 22 to 70 years of age. We report that the human heart is characterized by a significant turnover of ventricular myocytes, ECs, and fibroblasts, physiologically and pathologically. Myocyte, EC, and fibroblast renewal is very high shortly after birth, decreases during postnatal maturation, remains relatively constant in the adult organ, and increases dramatically with age. From 20 to 78 years of age, the adult human heart replaces entirely its myocyte, EC, and fibroblast compartment ~8, ~6, and ~8 times, respectively. Myocyte, EC, and fibroblast regeneration is further enhanced with chronic heart failure (CHF). Conclusions The human heart is a highly dynamic organ that retains a remarkable degree of plasticity throughout life and in the presence of CHF. However, the ability to regenerate cardiomyocytes, vascular ECs, and fibroblasts cannot prevent the manifestations of myocardial aging or oppose the negative effects of ischemic and idiopathic dilated cardiomyopathy.

show abstract

Section: Resultsmentioning

confidence: 99%

Cardiomyogenesis in the Aging and Failing Human Heart

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Seven sections were cut from each block. The first, fourth, and seventh (5 fim thickness) were set aside for histopathologic diagnosis (after hematoxylin-and-eosin staining), whereas the second and fifth (80 nm thickness) were subjected to a method described in detail elsewhere, 16 which enables cell suspensions to be obtained (after pronase digestion) that are then cytocentrifuged onto glass slides and stained with the Feulgen reaction. 16 …”

Section: Article Preparation Of Cell Suspensions Tissue Sections Anmentioning

confidence: 99%

“…Thus the DNA content of a given cell nucleus was obtained by summing the densitometric values of all the pixels included in its digitized image. 16 The DNA content of the cell population analyzed was obtained by summing the IOD values obtained for each of the 200 to 400 cell nuclei analyzed per case. The IOD enabled the DNA histogram type (DHT variable) to be determined.…”

mentioning

confidence: 99%

Image Cytometry Analysis of Feulgen-Stained Nuclei in 72 Lipomatous Lesions Including Atypical Lipomas and Well- Differentiated Liposarcomas

et al. 1996

View full text Add to dashboard Cite

Well-differentiated lipomatous tumors constitute a histopathologic category whose nomenclature has been controversial, particularly with respect to the distinction between atypical lipomas of the extremities and well-differentiated liposarcomas of the retroperitoneum. To determine whether there were differences in image analytic parameters between these neoplasms, 72 lesions including 21 typical lipomas, 7 atypical lipomas, 16 retroperitoneal and 5 nonretroperitoneal well-differentiated, 9 dedifferentiated, and 14 pleomorphic liposarcomas were submitted to the computer-assisted microscopic analysis of Feulgen-stained nuclei. This methodology enabled four groups of variables to be calculated. These included: (1) Myriam Remmelink holds a grant from the "Fondation Erasme," Brussels, Belgium.Robert Kiss is a Research Associate with the "Fonds National de la Recherche Scientifique" (FNRS), Belgium.Supported by grants from the "Fonds de la Recherche Scientifique Medicale" (FRSM), Belgium.Address reprint requests to Dr. Kiss: Laboratory of Histology, Faculty of Medicine, Free University of Brussels, 808 Route de Lennik, 1070 Brussels, Belgium.

show abstract

“…'5 Slides 2 and 6 were 80 jim thick and were sub- jected to a method that enables cell suspensions to be obtained (after pronase digestion). 16 They were cytocentrifuged on to glass slides and submitted to the Feulgen reaction'6 to determine the SPF index" (see below).…”

Section: Specimen Preparationsmentioning

confidence: 99%

Determination of proliferative activity in nasal polyps.

et al. 1997

Self Cite

View full text Add to dashboard Cite

Aims-To determine the level ofproliferative activity in 39 nasal polyps with clear cut distinct clinical behaviour patterns. Methods-The 39 nasal polyps included 11 polyps labelled as "single" and taken from the lateral nasal wall and the middle turbinate; 12 polyps labelled as "massive" and relating to diffuse polyposis involving the entire nasal cavity; six polyps labelled as "ASA" and relating to nasal polyps from patients with acetylsalicylic acid intolerance and asthma; and 10 polyps from cystic fibrosis related polyposis. Cell proliferation was determined by two independent methods: first, the computer assisted microscope analysis of isolated Feulgen stained nuclei for the measurement of the percentage of cells in the S phase of the cell cycle; and second, the immunohistochemical evaluation of a proliferation associated protein by means of the MIB 1 monoclonal antibody. Results-The cystic fibrosis related polyposis exhibited the highest proliferative activity of all the clinically identified nasal polyp groups. Acute inflammatory nasal polyps exhibited a higher cell proliferation than chronic ones. The results also show that while the immunohistochemical determination of cell proliferation by means of the MIB 1 monoclonal antibody is a valuable tool in determining cell proliferation in nasal polyps, the cytometrical image analysis of Feulgen stained nuclei is not useful for this purpose. Conclusion-Cell proliferation activity identifies cystic fibrosis as being distinct from the other nasal polyp groups. (7 Clin Pathol 1997;50:923-928)

show abstract

Characterization of factors in routine laboratory protocols that significantly influence the Feulgen reaction.

Cited by 57 publications

References 28 publications

Cardiomyogenesis in the Aging and Failing Human Heart

Cardiomyogenesis in the Aging and Failing Human Heart

Image Cytometry Analysis of Feulgen-Stained Nuclei in 72 Lipomatous Lesions Including Atypical Lipomas and Well- Differentiated Liposarcomas

Determination of proliferative activity in nasal polyps.

Contact Info

Product

Resources

About