Determination of proliferative activity in nasal polyps.

Abstract

Aims-To determine the level ofproliferative activity in 39 nasal polyps with clear cut distinct clinical behaviour patterns. Methods-The 39 nasal polyps included 11 polyps labelled as "single" and taken from the lateral nasal wall and the middle turbinate; 12 polyps labelled as "massive" and relating to diffuse polyposis involving the entire nasal cavity; six polyps labelled as "ASA" and relating to nasal polyps from patients with acetylsalicylic acid intolerance and asthma; and 10 polyps from cystic fibrosis related polyposis. Cell proliferation was determined by two independent methods: first, the computer assisted microscope analysis of isolated Feulgen stained nuclei for the measurement of the percentage of cells in the S phase of the cell cycle; and second, the immunohistochemical evaluation of a proliferation associated protein by means of the MIB 1 monoclonal antibody. Results-The cystic fibrosis related polyposis exhibited the highest proliferative activity of all the clinically identified nasal polyp groups. Acute inflammatory nasal polyps exhibited a higher cell proliferation than chronic ones. The results also show that while the immunohistochemical determination of cell proliferation by means of the MIB 1 monoclonal antibody is a valuable tool in determining cell proliferation in nasal polyps, the cytometrical image analysis of Feulgen stained nuclei is not useful for this purpose. Conclusion-Cell proliferation activity identifies cystic fibrosis as being distinct from the other nasal polyp groups. (7 Clin Pathol 1997;50:923-928)

Analysis of Ploidy in Hypopharyngeal Cancer by Laser Scanning Cytometry on Fine Needle Aspirate Biopsies

“…We conclude from this observation that LSC analysis of FNABs can be applied for the determination of the ploidy of a tumor pre-operatively. In our opinion LSC might have some advantages as compared to image cytometry: (A) with LSC much higher cell counts can easily be achieved than with image cytometry (about 300 cells for routine examinations) [18,31,39,40]; however, this increase in cell count very likely will not result in different ploidy values but rather in the detection of rare cells such as mitotic cells [12]. (B) In image cytometry cells are selected by the observer and therefore a substantial bias on the malignant cell population might result from preferring grossly atypical cells; this needs training in morphological analysis and helps to concentrate on the most interest-ing cells (i.e., the malignant cells) but could explain the sub-optimal correlation of the 5cER determined by image cytometry and LSC in our study.…”

Cell proliferation in nasal respiratory epithelium of people exposed to urbanpollution

“…Proliferative studies are reported for nasal polyps (65), and nasal inverted papillomas (66) and interestingly Kotelnikov et al (67) described the labeling index (LI)-as evaluated by bromodeoxyuridine-in nasal non-neoplasic pseudostratified epithelium in patients with head and neck tumors. The LI in four samples was 11.2%.…”

Age-related levels of fecal M2-pyruvate kinase in children with cystic fibrosis and healthy children 0 to 10 years old

“…In murine models this has been proposed to be due to increased cellular proliferation and/or reduced apoptosis [17,18]. In human studies increased cellular proliferation has been demonstrated in the airways and in nasal polyps in patients with CF compared with non-CF subjects [19][20][21]. Increased cellular turnover in the CF gut may be linked to an increased incidence of gastrointestinal malignancy [5,6], which becomes more relevant as the median age of survival in CF extends well into adulthood [22].…”