Monitoring of metabolic profiling and water status of Hayward kiwifruits by nuclear magnetic resonance

Capitani, Donatella; Mannina, Luisa; Proietti, Noemi; Sobolev, Anatoly P.; Tomassini, Alberta; Miccheli, Alfredo; Cocco, Maria Enrica Di; Capuani, Giorgio; Salvador, R. De; Delfini, Maurizio

doi:10.1016/j.talanta.2010.07.080

Cited by 59 publications

(48 citation statements)

References 47 publications

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“…The assignment of 1 H spectra performed in D 2 O and in D 2 O phosphate buffer (see Table ) was obtained by 1D and 2D NMR experiments, literature data and, when necessary, by addition of standard compounds.…”

Section: Resultsmentioning

confidence: 99%

NMR methodologies in the analysis of blueberries

et al. 2014

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An NMR analytical protocol based on complementary high and low field measurements is proposed for blueberry characterization. Untargeted NMR metabolite profiling of blueberries aqueous and organic extracts as well as targeted NMR analysis focused on anthocyanins and other phenols are reported. Bligh-Dyer and microwave-assisted extractions were carried out and compared showing a better recovery of lipidic fraction in the case of microwave procedure. Water-soluble metabolites belonging to different classes such as sugars, amino acids, organic acids, and phenolic compounds, as well as metabolites soluble in organic solvent such as triglycerides, sterols, and fatty acids, were identified. Five anthocyanins (malvidin-3-glucoside, malvidin-3-galactoside, delphinidin-3-glucoside, delphinidin-3-galactoside, and petunidin-3-glucoside) and 3-O-α-l-rhamnopyranosyl quercetin were identified in solid phase extract. The water status of fresh and withered blueberries was monitored by portable NMR and fast-field cycling NMR. (1) H depth profiles, T2 transverse relaxation times and dispersion profiles were found to be sensitive to the withering.

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Section: Resultsmentioning

confidence: 99%

NMR methodologies in the analysis of blueberries

et al. 2014

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“…Importantly, “Hongyang” also has a high content of vitamin C (136 mg 100 g −1 FW) and anthocyanin (2.99 mg 100 g −1 FW; Wang et al, 2003; Montefiori et al, 2005). Kiwifruit contains sugars such as glucose, fructose, and sucrose, and organic acids such as citric, quinic, malic, and ascorbic acids, and main amino acids such as glutamine, arginine, and aspartate (Redgwell and MacRae, 1992; Capitani et al, 2010). The combination of sugars, organic acids and free amino acids represents the major factor contributing to kiwifruit flavor (Sorrequieta et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit (Actinidia chinensis)

et al. 2016

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Genomic and transcriptomic data on kiwifruit (Actinidia chinensis) in public databases are very limited despite its nutritional and economic value. Previously, we have constructed and sequenced nine fruit RNA-Seq libraries of A. chinensis “Hongyang” at immature, mature, and postharvest ripening stages of fruit development, and generated over 66.2 million paired-end and 24.4 million single-end reads. From this dataset, here we have identified 7051 long noncoding RNAs (lncRNAs), 29,327 alternative splicing (AS) events and 2980 novel protein-coding genes that were not annotated in the draft genome of “Hongyang.” AS events were demonstrated in genes involved in the synthesis of nutritional metabolites in fruit, such as ascorbic acids, carotenoids, anthocyanins, and chlorophylls, and also in genes in the ethylene signaling pathway, which plays an indispensable role in fruit ripening. Additionally, transcriptome profiles and the contents of sugars, organic and main amino acids were compared between immature, mature, and postharvest ripening stages in kiwifruits. A total of 5931 differentially expressed genes were identified, including those associated with the metabolism of sugar, organic acid, and main amino acids. The data generated in this study provide a foundation for further studies of fruit development and ripening in kiwifruit, and identify candidate genes and regulatory elements that could serve as targets for improving important agronomic traits through marker assisted breeding and biotechnology.

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“…correlation (HSQC), Heteronuclear Multiple Bond Correlation (HMBC) and confirmed using an internal library of compounds and by comparison with literature data [19,20,21,22,23]. …”

Section: Discussionmentioning

confidence: 89%