13C NMR based profiling unveils different α-ketoglutarate pools involved into glutamate and lysine synthesis in the milk yeast Kluyveromyces lactis

Gorietti, D.; Zanni, Elena; Palleschi, Claudio; Delfini, Maurizio; Uccelletti, Daniela; Saliola, Michele; Puccetti, Caterina; Sobolev, Anatoly P.; Miccheli, A.

doi:10.1016/j.bbagen.2015.07.008

Cited by 7 publications

(6 citation statements)

References 41 publications

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“…In addition, Kluyveromyces marxianus is also ubiquitous in some RM French cheeses (Callon et al, 2006) and milk kefir grains from different regions in Italy (Garofalo et al, 2015). Kluyveromyces lactis is one of the most important non-Saccharomyces yeasts capable of growing on lactose as the sole carbon source, and is commonly used in various food and feed applications, genetic studies, and industrial applications (Gorietti et al, 2015). In this study, Kluyveromyces lactis was only found in Jk.…”

Section: Discussionmentioning

confidence: 86%

Microbial diversity in raw milk and traditional fermented dairy products (Hurood cheese and Jueke) from Inner Mongolia, China

et al. 2017

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Section: Discussionmentioning

confidence: 86%

Microbial diversity in raw milk and traditional fermented dairy products (Hurood cheese and Jueke) from Inner Mongolia, China

et al. 2017

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“…lactis model (42). Such model is recognized as a reference among the yeast community, as it serves as baseline for both experimental studies on metabolism and regulation towards biotechnological applications (43)(44)(45)(46)(47)(48)(49) and to build models of other yeast (50,51). The Streptococcus pneumoniae R6 (31) model has been reconstructed in merlin, having exciting results regarding the genome annotation and predictive capability.…”

Section: Validationmentioning

confidence: 99%

“…Moreover, the models' applications range from food biotechnology, drug targeting to biomaterials' production. The large extent and relevance of such applications are particularly endorsed by several published research articles (43)(44)(45)(46)(47) and different collaborations. Finally, the reconstruction of large-sized genome organisms such as Quercus suber highlights, unequivocally, merlin's scalability and quality.…”

Section: Validationmentioning

confidence: 99%

“…These studies confirm the reliability and robustness of merlin. Moreover, the applications range from food biotechnology to drug targeting and the production of biomaterials, whose relevance is particularly endorsed by several published articles (10)(11)(12)(13)(14)16) and different collaborations. Finally, reconstructing organisms with large genomes, such as Quercus suber, highlights merlin's scalability unequivocally.…”

Section: Merlin's Impact On Researchmentioning

confidence: 99%

“…Over the last years, merlin's user base has grown considerably, resulting in the reconstruction of GSMMs of multiple organisms from all domains of life, ranging from small-sized genome bacteria (7) to complex higher eukaryotes, such as the cork oak tree (8). Moreover, the Kluyveromyces lactis' model (9), developed and improved with merlin, is recognised as a reference among the yeast community, as it has served as the baseline for both experimental studies on metabolism and regulation towards biotechnological applications (10)(11)(12)(13)(14)(15)(16) and to build models of other yeasts (17,18). Herein, we present the newest version of merlin (version 4.0), which includes deep software architecture changes, database management, and significant updates in existing operations.…”

Section: Introductionmentioning

confidence: 99%

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merlinv4.0: an updated platform for the reconstruction of high-quality genome-scale metabolic models

Capela

Lagoa

Rodrigues

et al. 2021

Preprint

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Genome-scale metabolic models have been recognized as useful tools for better understanding living organism's metabolism. Merlin (https://merlin-sysbio.org/) is an open-source and user-friendly resource that hastens these models' reconstruction process, conjugating manual, and automatic procedures, while leveraging user's expertise with a curation-oriented graphical interface. An updated and redesigned version of merlin is herein presented. Since 2015, several features were implemented in merlin, along with profound changes in the software architecture, operating flow, and graphical interface. The current version (4.0) includes the implementation of novel algorithms and third-party tools for genome functional annotation, draft assembly, model refinement, and curation. Such updates led to an increase in the user-base, resulting in multiple published works including genome metabolic (re-)annotation and model reconstruction of multiple (lower and higher) eukaryotes and prokaryotes.

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Acetic acid acts as an elicitor exerting a chitosan-like effect on xanthone biosynthesis in Hypericum perforatum L. root cultures

et al. 2016

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Acetic acid acts as a signal molecule, strongly enhancing xanthone biosynthesis in Hypericum perforatum root cultures. This activity is specific, as demonstrated by the comparison with other short-chain monocarboxylic acids. We have recently demonstrated that Hypericum perforatum root cultures constitutively produce xanthones at higher levels than the root of the plant and that they respond to chitosan (CHIT) elicitation with a noteworthy increase in xanthone production. In the present study, CHIT was administered to H. perforatum root cultures using three different elicitation protocols, and the increase in xanthone production was evaluated. The best results (550 % xanthone increase) were obtained by subjecting the roots to a single elicitation with 200 mg l(-1) CHIT and maintaining the elicitor in the culture medium for 7 days. To discriminate the effect of CHIT from that of the solvent, control experiments were performed by administering AcOH alone at the same concentration used for CHIT solubilization. Unexpectedly, AcOH caused an increase in xanthone production comparable to that observed in response to CHIT. Feeding experiments with (13)C-labeled AcOH demonstrated that this compound was not incorporated into the xanthone skeleton. Other short-chain monocarboxylic acids (i.e., propionic and butyric acid) have little or no effect on the production of xanthones. These results indicate that AcOH acts as a specific signal molecule, able to greatly enhance xanthone biosynthesis in H. perforatum root cultures.

show abstract

13C NMR based profiling unveils different α-ketoglutarate pools involved into glutamate and lysine synthesis in the milk yeast Kluyveromyces lactis

Cited by 7 publications

References 41 publications

Microbial diversity in raw milk and traditional fermented dairy products (Hurood cheese and Jueke) from Inner Mongolia, China

Microbial diversity in raw milk and traditional fermented dairy products (Hurood cheese and Jueke) from Inner Mongolia, China

merlinv4.0: an updated platform for the reconstruction of high-quality genome-scale metabolic models

Acetic acid acts as an elicitor exerting a chitosan-like effect on xanthone biosynthesis in Hypericum perforatum L. root cultures

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