Monitoring of Japanese Encephalitis Virus Infection in Mosquitoes (Diptera: Culicidae) at Guandu Nature Park, Taipei, 2002–2004

Abstract

Japanese encephalitis virus (JEV) infection in mosquitoes was monitored at Guandu Nature Park in Taipei City from September 2002 to December 2004. In total, 30,386 female mosquitoes consisting of six genera and 14 species were processed for virus in 1,229 pools by using Flavivirus NS5 gene sequences detected by reverse transcription-polymerase chain reaction (PCR) and nested PCR assay. Overall, 101 pools were positive, including 95, 1, 4, and 1 for Culex tritaeniorhynchus Giles, Culex sitiens Wiedemann, Culex rubithoracis (Leicester), and Aedes vexans noctunmus (Theobald), respectively.

Mosquito Vector Competence for Japanese Encephalitis Virus

“…sitiens in East and South Asia, as well as in northern Oceania. The virus was found and/or isolated from mosquitoes in Malaysia [ 29 , 115 ], Taiwan [ 28 , 116 ], Papua New Guinea [ 118 ] and Australia [ 67 , 117 , 119 , 120 ]. In Australia, Cx .…”

“…In addition, JEV was detected over the last few decades in Cx . tritaeniorhynchus in Sri Lanka [ 52 ], India [ 39 , 53 , 62 , 63 , 64 , 65 , 66 , 68 , 69 , 99 , 100 , 101 , 121 , 122 , 126 ], Malaysia [ 29 ], Taiwan [ 116 ], Vietnam [ 106 ], South Korea [ 45 , 46 , 47 , 48 , 123 ], Taiwan [ 54 , 124 ], Japan [ 125 ] and China [ 85 , 127 , 128 ]. Simultaneously to the JEV isolation, Mitamura and colleagues also used Cx .…”