Monitoring of infection volatile markers using CMOS-based luminescent bioreporters

Ma, Junning; Veltman, Boris; Tietel, Zipora; Tsror, Leah; Liu, Yang; Eltzov, Evgeni

doi:10.1016/j.talanta.2020.121333

Cited by 14 publications

(7 citation statements)

References 68 publications

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“…The CMOS light signal measurement approach was used with a ULS24 solution kit with a 4-channel ultra-low-light CMOS image sensor. The CMOS approach presents the limitation of low signal-noise ratio in terms of bacterial bioluminescence measurement [ 8 ]. The second tested approach was the use of a smartphone that is more commonly available, together with high-performance CMOS sensors and assembly of optical lens.…”

Section: Resultsmentioning

confidence: 99%

“…The filter membrane disks were integrated with a CMOS sensor (Anitoa 4-Channel ULS24 Solution Kit, Menlo Park, CA, USA) to develop a remote and on-site detection of water toxicity [ 8 ]. Firstly, the disks were immobilized with the selected bacterial strain TV1061.…”

Section: Methodsmentioning

confidence: 99%

“…The data processing was conducted by using python (version 3.7.3) script ( Supplementary Material ). The data analysis included the following steps: (1) Extraction of temperature reading for each measurement; (2) data extraction for each channel; (3) calculation of background signal reading according to the standard curve [ 8 ]; (4) calibration of the bacterial signal by the subtraction of the calculated background signal; (5) computation of the induction factor; (6) and reconstruction of the output images.…”

Section: Methodsmentioning

confidence: 99%

“…Other entrapment-encapsulation based immobilization approaches demonstrated higher activity of the immobilized cells as compared to the planktonic cells. Such examples include the immobilization of bacteria cells in gel-like matrices (e.g., sodium alginate and agar-agar) or on surfaces that enable the creation of a biofilm (e.g., optical fiber, gold, glass, and polystyrene) [ 8 , 9 , 10 , 11 , 12 ]. Bacteria cells that are immobilized in a biofilm state can display distinct physiological characteristics from that in the free-floating state [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Smartphone-Based Whole-Cell Biosensor Platform Utilizing an Immobilization Approach on a Filter Membrane Disk for the Monitoring of Water Toxicants

Harpaz

Liu

et al. 2020

Sensors

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Bioluminescent bacteria whole-cell biosensors (WCBs) have been widely used in a range of sensing applications in environmental monitoring and medical diagnostics. However, most of them use planktonic bacteria cells that require complicated signal measurement processes and therefore limit the portability of the biosensor device. In this study, a simple and low-cost immobilization method was examined. The bioluminescent bioreporter bacteria was absorbed on a filter membrane disk. Further optimization of the immobilization process was conducted by comparing different surface materials (polyester and parafilm) or by adding glucose and ampicillin. The filter membrane disks with immobilized bacteria cells were stored at −20 °C for three weeks without a compromise in the stability of its biosensing functionality for water toxicants monitoring. Also, the bacterial immobilized disks were integrated with smartphones-based signal detection. Then, they were exposed to water samples with ethanol, chloroform, and H2O2, as common toxicants. The sensitivity of the smartphone-based WCB for the detection of ethanol, chloroform, and H2O2 was 1% (v/v), 0.02% (v/v), and 0.0006% (v/v), respectively. To conclude, this bacterial immobilization approach demonstrated higher sensitivity, portability, and improved storability than the planktonic counterpart. The developed smartphone-based WCB establishes a model for future applications in the detection of environmental water toxicants.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Smartphone-Based Whole-Cell Biosensor Platform Utilizing an Immobilization Approach on a Filter Membrane Disk for the Monitoring of Water Toxicants

Harpaz

Liu

et al. 2020

Sensors

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“…Some experimental data show that there is a certain correlation and comparability between IC 50 values obtained from cell-based experiments and animal experiments . Many kinds of water toxicity biosensors based on luminescent bacteria have been developed in recent decades . However, it is necessary to add 3% NaCl to the test solution, which causes a change in the composition of the test solution and introduces detection errors .…”

mentioning

confidence: 99%

Colorimetric and Electrochemical Dual-Signal Method for Water Toxicity Detection Based on Escherichia coli and p-Benzoquinone

Sun

et al. 2021

ACS Sens.

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The development of simple and rapid toxicity detection methods has important practical significance. In this work, a dual-signal method with colorimetric and electrochemical properties for water toxicity detection was proposed for the first time based on a rapid color reaction between Escherichia coli (E. coli) and p-benzoquinone (BQ). Here, E. coli was used as a biocatalyst and BQ was used as a mediator. An IC50 value of 0.75 mg L–1 for Cu2+ was obtained using a two-step electrochemical detection method. Strikingly, toxicity could also be estimated visually by the naked eye, and the minimum detection limit was 3.2 mg L–1 for Cu2+. The dual-signal toxicity detection method extends the function of BQ, and the result is more reliable than the traditional single-signal method. This simple and rapid toxicity detection method shows certain application prospects.

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Portable light detectors for bioluminescence biosensing applications: A comprehensive review from the analytical chemist's perspective

Calabretta

Lopreside

Montali

et al. 2022

Analytica Chimica Acta

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Monitoring of infection volatile markers using CMOS-based luminescent bioreporters

Cited by 14 publications

References 68 publications

Smartphone-Based Whole-Cell Biosensor Platform Utilizing an Immobilization Approach on a Filter Membrane Disk for the Monitoring of Water Toxicants

Smartphone-Based Whole-Cell Biosensor Platform Utilizing an Immobilization Approach on a Filter Membrane Disk for the Monitoring of Water Toxicants

Colorimetric and Electrochemical Dual-Signal Method for Water Toxicity Detection Based on Escherichia coli and p-Benzoquinone

Portable light detectors for bioluminescence biosensing applications: A comprehensive review from the analytical chemist's perspective

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