Monitoring of ajmaline in plasma with high-performance liquid chromatography

Liquid chromatographic assay with fluorescence detection to determine ajmaline in serum from patients with suspected Brugada syndrome

“…It is the first completely validated bioanalytical assay for this chemically very stable drug. The assay is a factor two more sensitive than the only other method using the same analytical technique and protein precipitation [8]. The assay is now successfully implemented for pharmacokinetic-pharmacodynamic relationship evaluation of the drug and the ECG data in the challenge test of patients with a (potential) Brugada syndrome.…”

“…The first assay that was useful for pharmacokinetic evaluation of the drug was reported by Clemans et al [6] using GC-MS after an extremely tedious sample pre-treatment using five liquid-liquid extraction steps and derivatization. Easier assays all use liquid chromatography with fluorescent detection after an ethereal liquid-liquid extraction [7,8], automated solid-phase extraction [9] or on-line extraction [10] procedure. In addition, protein precipitation with acetonitrile was investigated shortly as an alternative for liquid-liquid extraction by Koppel et al [8].…”

“…Easier assays all use liquid chromatography with fluorescent detection after an ethereal liquid-liquid extraction [7,8], automated solid-phase extraction [9] or on-line extraction [10] procedure. In addition, protein precipitation with acetonitrile was investigated shortly as an alternative for liquid-liquid extraction by Koppel et al [8]. Despite its poorer sensitivity this can be a useful, fast and easy option for pharmacokinetic evaluations.…”

Analyzing histological material to determine ajmaline and other drugs using high‐performance liquid chromatography/tandem mass spectrometry

“…[1][2][3][4][5][6] But only the verification of sildenafil in tissues fixed in formalin by gas chromatography-mass spectrometry (GC-MS) has been described yet. Consequently, an analysis of ajmaline in histological samples was necessary.…”

“…Different methods for determining ajmaline in serum, blood, urine, and postmortem material are well known. [1][2][3][4][5][6] But only the verification of sildenafil in tissues fixed in formalin by gas chromatography-mass spectrometry (GC-MS) has been described yet. 7 Hence, a method had to be developed to analyze drugs in histological materials by liquid chromatography-tandem mass spectrometry (LC-MS/MS).…”