Analyzing histological material to determine ajmaline and other drugs using high‐performance liquid chromatography/tandem mass spectrometry

Henning, Katarina; Dziadosz, Marek; Klintschar, Michael; Piep, Birgit; Teske, Jörg

doi:10.1002/dta.2450

Cited by 2 publications

(4 citation statements)

References 7 publications

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“…Regarding the extraction, isolation and purification of the analytes from these embedded matrices, we investigated several procedures to establish an effective method suitable for our purposes. While the grinding of the embedded samples into small pieces was a procedure in common with similar toxicological research articles [11,12], the solubilization of the paraffin wax embedding the samples was a more delicate procedure. Classic deparaffinization involving the use of xylene or a xylene substitute and subsequent rehydrating passages in a decreasing alcoholic ladder [17] was considered as too invasive under a toxicological point of view; the washing out and subsequent loss of analytes had to be taken into consideration, and we therefore decided to proceed by solubilizing paraffin in a non-polar solvent n-hexane.…”

Section: Discussionmentioning

confidence: 99%

“…However this study consisted of solely qualitative evaluations of the results. Another study [11], assessed in 2018, highlighted the presence of ajmaline in histological samples of liver and heart, but this study as well was evaluated only under a qualitative point of view and nothing about the details of the method was given.…”

Section: Discussionmentioning

confidence: 99%

“…These embedded samples have revealed themselves of great value for several analysis; DNA and RNA extractions can be carried out on these samples [6,7] as well as proteomic studies [8] and western-blot analysis [9]. Toxicological extractions on this type of samples, at this moment, have been attempted in a few cases [10][11][12], but a valid extractive procedure has not been established yet. 1 3 International guidelines [13] specify the type of sampling of organs and tissues that must be assessed on a corpse during autopsy examination and that are related to the different judiciary investigations that have to be carried out.…”

Section: Introductionmentioning

confidence: 99%

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Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago

et al. 2022

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Purpose Since the solely certain remnants of a performed autopsy are formalin-fixed paraffin-embedded (FFPE) samples, stored in the archives of every institute of legal medicine, we managed to extract molecules of toxicological interest from these specimens. Methods We assessed the analysis of ten fresh liver samples collected from heroin-related deaths and then histologically processed the same samples. The embedded blocks were then extracted by means of a new extracting method and the eluates were measured. We also selected five toxicological cases of heroin-related fatalities that were examined 20 years ago, collected the toxicological result documents of the analysis that were carried out at the time and then processed the corresponding FFPE liver samples that were stored in the archives. Results We managed to isolate heroine-related metabolites from 20-year-old paraffin-embedded blocks and calculated ratios to evaluate the performance of our new extraction. Conclusions According to our study, it is feasible to carry out a toxicological examination on old histological samples and, therefore, this matrix can be considered as a new alternative specimen for chemical-analytical evaluations of past cases or when fresh samples are not available anymore. The new extractive method was evaluated as efficient in treating these complex, paraffin-embedded samples. It was surprising that the target compounds could be quantitated from FFPE bocks created as long as 20 years ago.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago

et al. 2022

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“…However, the details of the procedure are not explained, and it is not specified whether only one swab was used for both DNA and toxicological analyses [1]. Other studies have shown that it is possible to combine two different forensic disciplines on paraffin-embedded blocks: these substrates have been used to perform both DNA [2] and toxicological investigations [3][4][5][6] as well as proteomics [7] and western-blot studies [8]. However, no studies reported the combination of DNA investigations and toxicological analyses when only a single sample is available.…”

Section: Introductionmentioning

confidence: 99%

Chemical investigation of biological trace evidence; toxicological screening of waste residues obtained from DNA extraction processes

Di Candia,

Giordano,

Boracchi

et al. 2023

Int J Legal Med

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In a forensic scenario, if biological stains are found in very small quantities, these are usually collected for DNA analyses, considered essential for the forensic investigation and thus excluding possible investigations by other forensic disciplines as forensic toxicology. We developed an experimental study to evaluate the feasibility of analyzing DNA extraction residues obtained from DNA extraction procedures to perform toxicological analysis, with the aim to extract both genetic and toxicological information without affecting or compromising the genetic sample and/or DNA extraction. DNA extraction from four blood samples (fortified with 5 molecules of interest with a final concentrations of 1 µg/mL, 100 ng/mL, 10 ng/mL and 5 ng/mL, respectively) were analyzed with QIAGEN QIAmp® DNA Mini kit. Three waste residues collected from the DNA extraction were analyzed for the toxicological investigation via Solid-Phase Extraction and High-Performance Liquid Chromatography—Tandem Mass Spectrometry analyses (Thermo Scientific™ TSQ Fortis™ II Triple-Quadrupole Mass Spectrometer). The analytical investigation revealed that our analytes of interest were detected in two different residues of the DNA extraction procedure, allowing both genetic and toxicological analyses without affecting the DNA identification. At last, the experimental protocol was applied to a hypothetical case, with encouraging results and allowing the identification of our molecules of interest.

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Analyzing histological material to determine ajmaline and other drugs using high‐performance liquid chromatography/tandem mass spectrometry

Cited by 2 publications

References 7 publications

Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago

Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago

Chemical investigation of biological trace evidence; toxicological screening of waste residues obtained from DNA extraction processes

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