Monitoring Mycoparasitism of Clonostachys rosea against Botrytis cinerea Using GFP

Hasan, Rabiul; Lv, Binna; Uddin, Md. Jamal; Chen, Yingying; Fan, Lele; Sun, Zhan‐Bin; Sun, Man-Hong; Li, Shidong

doi:10.3390/jof8060567

Cited by 14 publications

(6 citation statements)

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“…By contrast, antifungal activities were greatly reduced for CrGlu6-deficient mutants, and control efficacy against soybean rot was significantly decreased in the greenhouse. Research on the direct interaction of the mycoparasite and its prey, including recognition, enwind, penetration, and disintegration will be very interesting [69]. Research on these phenotypes is underway to explore the mechanisms connecting the CrGlu6 gene to the vegetative growth and biocontrol activities of C. chloroleuca.…”

Section: Discussionmentioning

confidence: 99%

Glucose-6-phosphate 1-Epimerase CrGlu6 Contributes to Development and Biocontrol Efficiency in Clonostachys chloroleuca

Guo

Zhang

et al. 2023

JoF

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Clonostachys chloroleuca (formerly classified as C. rosea) is an important mycoparasite active against various plant fungal pathogens. Mitogen-activated protein kinase (MAPK) signaling pathways are vital in mycoparasitic interactions; they participate in responses to diverse stresses and mediate fungal development. In previous studies, the MAPK-encoding gene Crmapk has been proven to be involved in mycoparasitism and the biocontrol processes of C. chloroleuca, but its regulatory mechanisms remain unclear. Aldose 1-epimerases are key enzymes in filamentous fungi that generate energy for fungal growth and development. By protein–protein interaction assays, the glucose-6-phosphate 1-epimerase CrGlu6 was found to interact with Crmapk, and expression of the CrGlu6 gene was significantly upregulated when C. chloroleuca colonized Sclerotinia sclerotiorum sclerotia. Gene deletion and complementation analyses showed that CrGlu6 deficiency caused abnormal morphology of hyphae and cells, and greatly reduced conidiation. Moreover, deletion mutants presented much lower antifungal activities and mycoparasitic ability, and control efficiency against sclerotinia stem rot was markedly decreased. When the CrGlu6 gene was reinserted, all biological characteristics and biocontrol activities were recovered. These findings provide new insight into the mechanisms of glucose-6-phosphate 1-epimerase in mycoparasitism and help to further reveal the regulation of MAPK and its interacting proteins in the biocontrol of C. chloroleuca.

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Section: Discussionmentioning

confidence: 99%

Glucose-6-phosphate 1-Epimerase CrGlu6 Contributes to Development and Biocontrol Efficiency in Clonostachys chloroleuca

Guo

Zhang

et al. 2023

JoF

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“…Clonostachys rosea is a hyperparasitic fungus capable of invading various plant-pathogenic fungi, including Botrytis cinerea, Fusarium spp., Rhizoctonia solani, and Sclerotinia sclerotiorum (Barnett and Lilly, 1962;Cota et al ., 2008;Jensen et al ., 2000;Luongo et al ., 2005;Rodríguez et al ., 2011), with C. rosea strain 67-1 being highly efficient for biocontrol (Zhang et al ., 2007;Ma et al ., 2011;Sun et al ., 2018). Hasan et al (2022) showed that the GFP-marked C. rosea strain 67-1 exerts antagonistic activities against B. cinerea both in vitro and on tomato leaves. The hyperparasite is able to penetrate its host, absorb its nutrients, and eventually disintegrate all of its cells.…”

Section: Hyperparasitic Fungi and Biological Controlmentioning

confidence: 98%

Hyperparasitic fungi—definitions, diversity, ecology, and research

Bermúdez-Cova,

Haelewaters,

Bekker

et al. 2023

Preprint

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Even parasites have parasites. By definition, a hyperparasite is an organism capable of parasitizing another parasite. Hyperparasitism caused by fungi is a common phenomenon in nature, but it has been poorly studied. This life history strategy evolved several times in the fungal tree of life, and is crucial in the maintenance of ecosystems as well as in the mediation of parasite–host interactions. Although the interest for hyperparasitic fungi is growing in the context of biological control, hyperparasitism is not ecologically and evolutionarily understood. This chapter summarizes the most relevant aspects of the terminology, diversity, and ecology of hyperparasitic fungi on both fungal and non-fungal hosts. We also discuss the problems related to molecular research on hyperparasitic fungi. As they represent a hidden source of diversity, it is necessary to increase sampling efforts and to undertake further morphological, molecular, and ecological studies to understand these fungi and their potential biotechnological and pharmaceutical uses.

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“…Furthermore, Zhang et al (2020) [38] first reported on the possibility of root rot in saffron plants specifically caused by P. solitum. C. rosea was previously used as a biocontrol agent because of its active mycoparasitism [39,40]. However, in recent years, researchers identified root rot in grain legumes and medicinal crops caused by C. rosea [41][42][43][44].…”

Section: Introductionmentioning

confidence: 99%

Introducing Autochthonous Bacterium and Fungus Composition to Enhance the Phytopathogen-Suppressive Capacity of Composts against Clonostachys rosea, Penicillium solitum and Alternaria alternata In Vitro

Mironov,

Shchelushkina,

Selitskaya

et al. 2023

Agronomy

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Given their numerous positive characteristics, composts are widely used agriculturally in sustainable development and resource-saving technologies. The management of phytopathogen-suppressive potential and the fertilizing capacity of composts are of great interest. This study examines the impact of introducing the autochthonous compost species Bacillus subtilis, B. amyloliquefaciens, Pseudomonas aeruginosa, and Aspergillus corrugatus, both individually and in combination, to composts containing dry matter comprising 36% solid compost and 7% compost suspensions to study their phytopathogen-suppressive and phytostimulation activity. The test phytopathogens were Clonostachys rosea, Penicillium solitum, and Alternaria alternata. This is the first report on compost’s potential to biologically control C. rosea and P. solitum. Classical microbiological and molecular biological methods were used to evaluate the survival rate of microorganisms in compost and validate these results. Test plant (Raphanus sativus) germination indexes were determined to evaluate the phytotoxic/phytostimulation effects of the substrates. To assess the effectiveness of biocontrol, mycelial growth inhibition was measured using in vitro tests. The introduction of composition increased the composts’ fertilizing properties by up to 35% and improved antagonistic activity by up to 91.7%. Autochthonous bacterial–fungal composition can promote resistance to fungal root and foliar phytopathogens and raise the fertilizing quality of compost.

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Monitoring Mycoparasitism of Clonostachys rosea against Botrytis cinerea Using GFP

Cited by 14 publications

References 58 publications

Glucose-6-phosphate 1-Epimerase CrGlu6 Contributes to Development and Biocontrol Efficiency in Clonostachys chloroleuca

Glucose-6-phosphate 1-Epimerase CrGlu6 Contributes to Development and Biocontrol Efficiency in Clonostachys chloroleuca

Hyperparasitic fungi—definitions, diversity, ecology, and research

Introducing Autochthonous Bacterium and Fungus Composition to Enhance the Phytopathogen-Suppressive Capacity of Composts against Clonostachys rosea, Penicillium solitum and Alternaria alternata In Vitro

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