Monitoring Key Parameters in Bioprocesses Using Near-Infrared Technology

Abstract

Near-infrared spectroscopy (NIRS) is known to be a rapid and non-destructive technique for process monitoring. Bioprocesses are usually complex, from both the chemical (ill-defined medium composition) and physical (multiphase matrix) aspects, which poses an additional challenge to the development of robust calibrations. We investigated the use of NIRS for on-line and in-line monitoring of cell, substrate and product concentrations, during aerobic and anaerobic bacterial fermentations, in different fermentation strategies. Calibration models were built up, then validated and used for the automated control of fermentation processes. The capability of NIR in-line to discriminate among differently shaped bacteria was tested.

Development of FT-NIR Models for the Simultaneous Estimation of Chlorophyll and Nitrogen Content in Fresh Apple (Malus Domestica) Leaves

“…NIRS-based technology is widespread in different industrial sectors, from pharmaceuticals [ 23 ] to food [ 24 ] and bioprocesses [ 25 ]. Up to now, applications of NIR to orchards have been only focused on the analysis of apple-fruit firmness, ripening and sugar content, or they have been oriented to post-harvest quality control, in combination with visible spectroscopy but only exploiting the narrow range 12,500–10,000 cm −1 [ 26 , 27 ].…”

NIR spectroscopy applied to the determination of 2‐phenylethanol and l‐phenylalanine concentrations in culture medium of Yarrowia lipolytica

“…The main advantages of near‐infrared (NIR) spectroscopy over reference methods are its speed, both non‐destructive and non‐contaminant nature, and great accuracy . Bioprocesses are usually complex, both from the chemical (ill‐defined medium composition) and physical (multiphase matrix) aspects, which poses an additional challenge to the development of robust calibrations . As a result, different studies have been conducted to apply NIR to the monitoring of bioprocesses in general .…”

Real‐time monitoring of ethanol production during Pichia stipitis NRRL Y‐7124 alcoholic fermentation using transflection near infrared spectroscopy

“…Miguel A. de Quevedo 2779, Col. Formando Hogar, CP 91860, Veracruz, México Abbreviation: %e, error percentage; MSC, multiplicative scattering correction; NIR, near-infrared; NIRS, near-infrared spectroscopy; PLSR, partial least square regression; VIP, variable influence in the projection of several advantages [1]; however, a major drawback of this species is that it cannot metabolize xylose [2], the most common sugar in hemicellulose and which represents a sizable fraction of lignocellulosic materials. Hence, near infrared spectroscopy (NIRS) can be applied as a real-time fermentation monitoring methodology using multiconstituent, rapid and nondestructive analyses, without involving sample pretreatment that leads to effective bioprocess control, a tool for increased yield, productivity, and reproducibility [14][15][16]. Nevertheless, the use of yeasts such as Pichia stipitis, naturally capable of metabolizing hexoses and pentoses, is a feasible solution for ethanol production from these renewable resources.…”

“…Development and bioprocess optimization are highly dependent on accurate real-time monitoring of chemical and physical process variables [11][12][13]. Hence, near infrared spectroscopy (NIRS) can be applied as a real-time fermentation monitoring methodology using multiconstituent, rapid and nondestructive analyses, without involving sample pretreatment that leads to effective bioprocess control, a tool for increased yield, productivity, and reproducibility [14][15][16]. In the present study, the utility of NIRS for real-time monitoring of alcoholic fermentation by P. stipitis NRRL Y-7124, using glucose and xylose as a carbon source and the use of chemometric tools and plots for understanding the process, was investigated.…”