Monitoring intracellular pH fluctuation with an excited-state intramolecular proton transfer-based ratiometric fluorescent sensor

An ESIPT-based AIE fluorescent probe to visualize mitochondrial hydrogen peroxide and its application in living cells and rheumatoid arthritis

“…HTQ exhibited maximum absorption at about 400 nm in most organic solvents, but showed another absorption peak at 520 nm in N , N -dimethylformamide (DMF) and dimethyl sulfoxide (DMSO). The latter one can be attributed to the deprotonation of the hydroxyl group in DMF or DMSO, 44 in which the deprotonation form resulted in intensified ICT strength in the molecule. In addition, HTQ exhibited an absorption peak at 420 nm in PBS.…”

Excited-state intramolecular proton transfer (ESIPT)-based fluorescent probes for biomarker detection: design, mechanism, and application

“…Feng et al synthesized a ratiometric fluorescent sensor, Probe 1 , for pH sensing based on the amino-type ESIPT process. 144 It is a promising sensor that responds rapidly and reversibly to pH changes after the introduction of a strong electron-withdrawing tosyl group. Probe 1 had a large Stokes shift in an acidic medium exhibiting yellow emission.…”

Colorimetric detection of ozone in aqueous solution and imaging in living cells using a novel hemicyanine dye

“…8,9 Fluorescent probes are a class of molecules that can identify analytes by their fluorescence signals and realize the qualitative or quantitative analysis of an analyte. Due to their advantages of high sensitivity, good selectivity, wide application range, and low cost, they have been widely used in the determination of various analytes 10–15 in recent years. Therefore, to overcome the above shortcomings in current O 3 detection, fluorescent probes are preferentially used to detect the concentration of O 3 in environmental and biological media.…”