Monitoring Diet Effects via Biofluids and Their Implications for Metabolomics Studies

Gu, Haiwei; Chen, Huanwen; Pan, Zhengzheng; Jackson, Ayanna U.; Talaty, Nari; Xi, Bowei; Kissinger, Candice B.; Duda, Chester T.; Mann, D. D.; Raftery, Daniel; Cooks, R. Graham

doi:10.1021/ac060946c

Cited by 100 publications

(90 citation statements)

References 41 publications

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“…Decreased levels of urinary xanthosine are found in rats with myocardial infarction compared with controls (61). Also, xanthosine is a discriminant compound indicating different diets found in the urine of rats fed by a normal diet and a turkey-based diet (62). On the other hand, proline betaine is a marker of citrus consumption based on orange juice intake (63) .…”

Section: Food Intake Metabolites (Food Metabolome) Particular For Eacmentioning

confidence: 99%

Metabolomic Pattern Analysis after Mediterranean Diet Intervention in a Nondiabetic Population: A 1- and 3-Year Follow-up in the PREDIMED Study

et al. 2014

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The Mediterranean diet (MD) is considered a dietary pattern with beneficial effects on human health. The aim of this study was to assess the effect of an MD on urinary metabolome by comparing subjects at 1 and 3 years of follow-up, after an MD supplemented with either extra-virgin olive oil (MD+EVOO) or nuts (MD+Nuts), to those on advice to follow a control low-fat diet (LFD). Ninety-eight non-diabetic volunteers were evaluated, using metabolomic approaches, corresponding to MD+EVOO (n=41), MD+Nuts (n=27) or LFD (n=30) groups. The 1 H-NMR urinary profiles were examined at baseline, after 1 and 3 years of follow-up. Multivariate data analysis (OSC-PLS-DA and HCA) methods were used to identify the potential biomarker discriminating groups, exhibiting a urinary metabolome separation between MD groups against baseline and LFD. Results revealed that the most prominent hallmarks concerning MD groups were related to the metabolism of carbohydrates (3-hydroxybutyrate, citrate, cis-aconitate), creatine, creatinine, amino acids (proline, Nacetylglutamine, glycine, branched-chain amino acids and derived metabolites), lipids(oleic and suberic acids), and microbial co-metabolites (phenylacetylglutamine, pcresol). Otherwise, hippurate, trimethylamine-N-oxide, histidine and derivates (methylhistidines, carnosine, anserine) and xanthosine were predominant after LFD. The application of NMR-based metabolomics enabled the classification of individuals regarding their dietary pattern and highlights the potential of this approach for evaluating changes in the urinary metabolome at different time points of follow-up in response to specific dietary interventions.2

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Section: Food Intake Metabolites (Food Metabolome) Particular For Eacmentioning

confidence: 99%

Metabolomic Pattern Analysis after Mediterranean Diet Intervention in a Nondiabetic Population: A 1- and 3-Year Follow-up in the PREDIMED Study

et al. 2014

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“…This results in a high tolerance of EESI for complex matrices. For example, untreated biological materials such as raw urine or milk can be directly infused to generate constant signal for hours [66,68,69]. Another significant merit of EESI is that the sample is isolated from any high voltage and can be located far way from the EESI source.…”

Section: Samples Accessible For Analysis By Ambient Ionization Technimentioning

confidence: 99%

What can we learn from ambient ionization techniques?

Chen

Gamez

Zenobi

2009

J. Am. Soc. Mass Spectrom.

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227

178

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Ambient mass spectrometry-mass spectrometric analysis with no or minimal effort for sample preparation-has experienced a very rapid development during the last 5 years, with many different methods now available for ionization. Here, we review its range of applications, the hurdles encountered for its quantitative use, and the proposed mechanisms for ion formation. Clearly, more effort needs to be put into investigation of matrix effects, into defining representative sampling of heterogeneous materials, and into understanding and controlling the underlying ionization mechanisms. Finally, we propose a concept to reduce the number of different acronyms describing very similar embodiments of ambient mass spectrometry. (J Am Soc Mass

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“…The successes of DESI in a large variety of applications [4,6,[15][16][17][18][19] are partly due to the availability of various surfaces from which the sample can be analyzed and, even more important, due to the ability to optimize the spray solvent for the analyte(s) of interest. Addition of a selective reagent to the solvent spray can be used to enhance the analyte signal by an appropriate chemical reaction; an experiment that is termed reactive DESI [20 -22].…”

Section: Esorption Electrospray Ionization (Desi) Is Anmentioning

confidence: 99%

“…In highly concentrated samples (Ͼ10 Ϫ5 M), there is competition among analyte ions during droplet formation for outer surface space and this is believed to contribute to ion suppression [11]. Ion suppression is also thought to occur due to the masking of analytes by matrix ions of greater polarity or even by high mass compounds masking lower mass analytes [10,12].The successes of DESI in a large variety of applications [4,6,[15][16][17][18][19] are partly due to the availability of various surfaces from which the sample can be analyzed and, even more important, due to the ability to optimize the spray solvent for the analyte(s) of interest. Addition of a selective reagent to the solvent spray can be used to enhance the analyte signal by an appropriate chemical reaction; an experiment that is termed reactive DESI [20 -22].…”

mentioning

confidence: 99%

Salt tolerance of desorption electrospray ionization (DESI)

Jackson

Talaty

Cooks

et al. 2007

J. Am. Soc. Mass Spectrom.

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The salt tolerance of desorption electrospray ionization (DESI) was systematically investigated by examining three different drug mixtures in the presence of 0, 0.2, 2, 5, 10, and 20% NaCl:KCl (1:1) from different surfaces. At physiological salt concentrations, the individual drugs in each mixture were observed in each experiment. Even at salt concentrations significantly above physiological levels, particular surfaces were effective in providing spectra that allowed the ready identification of the compounds of interest in low nanogram amounts. Salt adducts, which are observed even in the absence of added salt, could be eliminated by adding 0. . The method involves electrospraying a solvent to generate charged microdroplets that impact a condensed-phase sample and extracts analyte molecules in situ. Secondary droplets containing analyte ions are sucked by the vacuum into the atmospheric sampling interface of a mass spectrometer. In various cases, complex biological mixtures have been analyzed successfully with little or no sample preparation [3][4][5][6][7][8][9]. In these and other previous studies, the preliminary indications were that DESI has a high salt tolerance. These studies did not explore the limits of this tolerance as a function of salt concentration, nor were the effects of the nature of the substrate or the possibility that additives in the spray solution might prevent the formation of salt adducts studied. Each of these enquiries is undertaken here.The underlying reasons for ion suppression effects has been a subject of interest in several electrospray ionization (ESI) mass spectrometry studies [10 -14]. A mechanistic investigation into this phenomenon concluded that it is more likely to occur in the liquid than in the gas phase, and that it can be ascribed to the presence of high concentrations of non-volatile compounds, such as salts and endogenous metabolites [10,[12][13][14]. Non-volatile materials like salts alter the efficiency of droplet formation, which in turn negatively affects the number of ions reaching the mass spectrometer. In highly concentrated samples (Ͼ10 Ϫ5 M), there is competition among analyte ions during droplet formation for outer surface space and this is believed to contribute to ion suppression [11]. Ion suppression is also thought to occur due to the masking of analytes by matrix ions of greater polarity or even by high mass compounds masking lower mass analytes [10,12].The successes of DESI in a large variety of applications [4,6,[15][16][17][18][19] are partly due to the availability of various surfaces from which the sample can be analyzed and, even more important, due to the ability to optimize the spray solvent for the analyte(s) of interest. Addition of a selective reagent to the solvent spray can be used to enhance the analyte signal by an appropriate chemical reaction; an experiment that is termed reactive DESI [20 -22]. By the inverse argument, possibly appropriate reagents added to the spray solution might reduce signals due to unwanted ions; such an action wou...

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Monitoring Diet Effects via Biofluids and Their Implications for Metabolomics Studies

Cited by 100 publications

References 41 publications

Metabolomic Pattern Analysis after Mediterranean Diet Intervention in a Nondiabetic Population: A 1- and 3-Year Follow-up in the PREDIMED Study

Metabolomic Pattern Analysis after Mediterranean Diet Intervention in a Nondiabetic Population: A 1- and 3-Year Follow-up in the PREDIMED Study

What can we learn from ambient ionization techniques?

Salt tolerance of desorption electrospray ionization (DESI)

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