Monitoring cell adhesion processes on bioactive polymers with the quartz crystal resonator technique

Strategies for cell manipulation and skeletal tissue engineering using high-throughput polymer blend formulation and microarray techniques

“…In this context, the physicochemical properties, such as hydrophobicity, roughness, formal charge and chemical composition are all known to play key roles in regulating cell-polymer reactivity [44][45][46][47][48]. Thus polymer materials lacking surface charge are known to mediate their effects on cell attachment by physiosorption of extracellular matrix (ECM) proteins [49].…”

Extracellular matrix remodelling during cell adhesion monitored by the quartz crystal microbalance

“…This complex, albeit flexible, process is dependent on culture conditions, including the underlying substrate and preadsorbed protein layer. The quartz crystal microbalance with dissipation monitoring (QCM-D) has been shown to be a very useful technique to monitor cell adhesion processes in real time [2][3][4]. Our previous studies have shown that cell adhesion can be monitored both in serum-containing and serum-free conditions [5] as well as serum-free conditions with preadsorbed proteins [6].…”

Measuring Cell Adhesion on RGD‐Modified, Self‐Assembled PEG Monolayers Using the Quartz Crystal Microbalance Technique

“…[10,11] However, compared to its use for protein adsorption experiments, only a few studies on cell adhesion using the QCM have been published. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] The different cell types that have been investigated lead to very different changes in resonance frequencies, depending on the cell seeding density, and of course on the different setups. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] A significant drawback of all those studies is that most of them were performed under static conditions (except for the study by Jenkins et al [18] ).…”