Extracellular matrix remodelling during cell adhesion monitored by the quartz crystal microbalance

Lord, Megan S.; Modin, Charlotte; Foss, Morten; Duch, Mogens; Simmons, Anne; Pedersen, Finn Skou; Besenbacher, Flemming; Milthorpe, Bruce

doi:10.1016/j.biomaterials.2008.03.002

Cited by 60 publications

(51 citation statements)

References 29 publications

(38 reference statements)

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“…The QCM-D technique focuses on the energy dissipation shift, 66,[92][93][94] allowing the monitoring of cell adhesion and spreading behaviors on surface-oxidized poly(styrene) (PSox), [95][96][97][98][99] tantalum (Ta), 98-100 chromium (Cr), 100 titanium (Ti) and steel. 101 The QCM-D technique has also been used to understand the effects of the pre-adsorption of proteins on cell adhesion; [98][99][100][101] for example, on Ta and Cr with and without the pre-adsorption of FBS 100 and on Ti, TiO 2 and steel with the pre-adsorption of Fn or Fgn. 101 Initial cell adhesion processes and changes in interfacial viscoelasticity using the QCM-D technique have been reported.…”

Section: Protein-mediated Cell Adsorption Formation Of Nano-bio Intermentioning

confidence: 99%

In situ QCM-D study of nano-bio interfaces with enhanced biocompatibility

Tagaya

2015

Polym J

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When biomaterials are implanted into an animal body, the body fluid proteins initially adsorb and then cells recognize these surfaces. Adherent cell functions respond differently to diverse biomaterial surfaces with different properties. Thus, an understanding of cellular responses to biomaterials is crucial for effective control of biomaterial − cell interactions. I have researched how to clarify interfacial phenomena via protein adsorption and subsequent cell adhesion to hydroxyapatite nanocrystals using a quartz crystal microbalance with a dissipation technique. In this review, I focused on the current understanding of enhanced biocompatibility by exploring the roles of protein mediation at the interface. The most promising nano-bio interfaces are explained, and different protein adsorption and cell adhesion processes are highlighted depending on their interfacial states. This approach will clarify several ambiguities of interfacial phenomena between biomaterials and cells and will help in the design of novel biomaterials that can be implanted.

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Section: Protein-mediated Cell Adsorption Formation Of Nano-bio Intermentioning

confidence: 99%

In situ QCM-D study of nano-bio interfaces with enhanced biocompatibility

Tagaya

2015

Polym J

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“…not reusable (Fohlevova et al, 2007;Guo et al, 2008;Lord et al, 2008;Wegener et al, 1998Wegener et al, , 2000Welle et al, 2007). In our study, we have described and validated a simple, novel piezoelectric methodology based on resistance-sensitive piezoelectric transduction, to detect the presence of viable cells and to measure the rate of cell division.…”

Section: Discussionmentioning

confidence: 99%

An alternative quantitative acoustical and electrical method for detection of cell adhesion process in real‐time

Guillou-Buffello

Gindre

Johnson

et al. 2010

Biotech & Bioengineering

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Sauerbrey [(1956), Z Phys 55:206-222] showed that the shift in resonance frequency of thickness shear mode (TSM) of a quartz crystal sensor is proportional to the mass, which is deposited on it. However, new powerful electrical circuits were developed that are capable of operating TSM quartz crystal sensors in fluids which enabled this method to be introduced into electrochemical and biological applications. These applications include the detection of virus capsids, bacteria, mammalian cells, the interaction of DNA and RNA with complementary strands, specific recognition of protein ligands by immobilized receptors, and last but not least the study of complete immunosensors. Piezoelectric quartz transducers allow a label-free identification of molecules; they are more than mass sensors since the biosensor response is also influenced by the surface charge of adsorbed proteins, interfacial phenomena, surface roughness and viscoelastic properties of the adhered biomaterial. These new characteristics have recently been used to investigate cell, liposome, and protein adhesion onto surfaces, thus permitting the rapid determination of morphological cell changes as a response to pharmacological substances, and changes in the water content of biopolymers avoiding of time-consuming methods. We validated an alternative quantitative acoustical engineering for cell adhesion process monitored by the TSM. Shear acoustical results (motional resistance) are further correlated to cell counting procedures and are sensitive of adhesion processes in real-time.

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“…The formation of pseudopodia would fall within the detection range and could therefore be the main cause of the increase in mass of the QCM-D chip leading to the frequency and dissipation shifts with time. Modin et al [13,24,25] also showed that QCM-D and consequently Df plots can be used to determine the spreading behaviour of cells through the increase in dissipation versus the decrease in frequency upon their contact with different surfaces even with the signal penetration depth limitation. Varying the experimental conditions such as cell type, buffer, temperature, etc., authors could obtain a typical Df plot for each of the given conditions [27] with a general trend of increasing slopes for D and decreasing slopes for f. This feature was then attributed to cell spreading, as cell spreading leads to a progressive increase of viscoelastic mass (owing to high hydration state of cells) on the sensor surface.…”

Section: Monitoring Platelet Activation Rate Using a Supporting Protementioning

confidence: 99%

“…QCM-D has proven to be a simple and effective way to quantitatively study cell adhesion and spreading in situ [13,24,25]. In a previous study [15], we also examined platelet adhesion in a quasithree-dimensional space via subtle cytoskeleton changes, with protein composition of the QCM-D chip biointerface.…”

Section: Introductionmentioning

confidence: 99%

“…Reports are available where reproducible dissipation factor (D) versus frequency ( f ) plots were obtained as a result of cell interactions with surfaces having various physico-chemical properties [13,[24][25][26][27][28]. Thrombogenic human fibronectin (HFN) and non-thrombogenic human serum albumin (HSA) have been employed as model protein biointerface to understand the biochemical processes involved in the QCM-D response during platelet activation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of surface-induced platelet activation by applying time-dependency dissipation factor versus frequency using quartz crystal microbalance with dissipation

Fatisson

Mansouri

Yacoub

et al. 2011

J. R. Soc. Interface.

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Platelet adhesion and activation rates are frequently used to assess the thrombogenicity of biomaterials, which is a crucial step for the development of blood-contacting devices. Until now, electron and confocal microscopes have been used to investigate platelet activation but they failed to characterize this activation quantitatively and in real time. In order to overcome these limitations, quartz crystal microbalance with dissipation (QCM-D) was employed and an explicit time scale introduced in the dissipation versus frequency plots (Df -t) provided us with quantitative data at different stages of platelet activation. The QCM-D chips were coated with thrombogenic and non-thrombogenic model proteins to develop the methodology, further extended to investigate polymer thrombogenicity. Electron microscopy and immunofluorescence labelling were used to validate the QCM-D data and confirmed the relevance of Df-t plots to discriminate the activation rate among protein-modified surfaces. The responses showed the predominant role of surface hydrophobicity and roughness towards platelet activation and thereby towards polymer thrombogenicity. Modelling experimental data obtained with QCM-D with a Matlab code allowed us to define the rate at which mass change occurs (A/B), to obtain an A/B value for each polymer and correlate this value with polymer thrombogenicity.

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Extracellular matrix remodelling during cell adhesion monitored by the quartz crystal microbalance

Cited by 60 publications

References 29 publications

In situ QCM-D study of nano-bio interfaces with enhanced biocompatibility

In situ QCM-D study of nano-bio interfaces with enhanced biocompatibility

An alternative quantitative acoustical and electrical method for detection of cell adhesion process in real‐time

Determination of surface-induced platelet activation by applying time-dependency dissipation factor versus frequency using quartz crystal microbalance with dissipation

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