Monitoring Biogenic Volatile Compounds Emitted by <i>Eucalyptus citriodora</i> Using SPME

Zini, Cláudia Alcaraz; Augusto, Fábio; Christensen, Eva; Smith, Benjamin; Caramão, Elina Bastos; Pawliszyn, Janusz

doi:10.1021/ac0103219

Cited by 80 publications

(63 citation statements)

References 28 publications

(42 reference statements)

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“…Analytes were desorbed in the injection port (2508C, 5 min) and analysed by GC-MS. Peak areas corresponding to the individual esters were normalized according to [2]:…”

Section: Resultsmentioning

confidence: 99%

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SPME – A valuable tool for investigation of flower scent

Barták¹,

Bednář²,

Čáp³

et al. 2003

J of Separation Science

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A novel Headspace Solid Phase Microextraction (HS-SPME) protocol is proposed for the analysis of floral scent. Volatile compounds emitted from the flower are collected on a Carboxen/PDMS fiber for 1 hour, transferred to the GC, and analyzed by GC/ MS. The method completely eliminates the use of organic solvents, does not require special instrumentation, and may readily be performed in the field without access to mains electricity and other energy supplies. The method is robust, sensitive, and reduces the sampling stress on the investigated plant. Since enzymatic reactions in living flowers may cause changes in the composition of emitted fragrance, dried rosemary (Rosmarinus officinalis L.) was used as a stable standard for the method development and optimization. In addition, grape wine was also suggested as homogeneous, bio-compatible, and relatively stable standard of pronounced and typical scent for the same purpose. The optimized method was used for the comparative investigation of the fragrances emitted by two different species -Lathyrus vernus (L.) and Orchis pallens (L.). Several monoterpenes (C10 compounds) were found as the main fragrance components of lathyrus, while sesquiterpenes (C15 compounds) were typical for the orchid.

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“…Analytes were desorbed in the injection port (2508C, 5 min) and analysed by GC-MS. Peak areas corresponding to the individual esters were normalized according to [2]:…”

Section: Resultsmentioning

confidence: 99%

“…Investigation of flower scent represents an important field of modern biological research directed towards special theories of biological recognition [1,2]. The scent of a flower together with its color are considered to be the main signal attracting insects ensuring pollination [3,4].…”

Section: Introductionmentioning

confidence: 99%

SPME – A valuable tool for investigation of flower scent

Barták¹,

Bednář²,

Čáp³

et al. 2003

J of Separation Science

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“…The main limitation of this method is the requirement of sophisticated instrumentation that is less convenient for field measurements (e.g., thermal desorption unit and cryofocusing). Solid-phase microextraction (SPME) has also been used for the collection of monoterpenes (e.g., Yassaa et al, 2010;Zini et al, 2001). This technique combines sampling and pre-concentration of analytes in a single step and allows for direct thermal desorption into a heated gas chromatograph injection port (Koziel et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Field measurements of biogenic volatile organic compounds in the atmosphere using solid-phase microextraction Arrow

Barreira¹,

Duporté

Rönkkö

et al. 2018

Atmos. Meas. Tech.

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Abstract. Biogenic volatile organic compounds (BVOCs) emitted by terrestrial vegetation participate in a diversity of natural processes. These compounds impact both shortrange processes, such as on plant protection and communication, and long-range processes, for example by participating in aerosol particle formation and growth. The biodiversity of plant species around the Earth, the vast assortment of emitted BVOCs, and their trace atmospheric concentrations contribute to the substantial remaining uncertainties about the effects of these compounds on atmospheric chemistry and physics, and call for the development of novel collection devices that can offer portability with improved selectivity and capacity. In this study, a novel solid-phase microextraction (SPME) Arrow sampling system was used for the static and dynamic collection of BVOCs from a boreal forest, and samples were subsequently analyzed on site by gas chromatography-mass spectrometry (GC-MS). This system offers higher sampling capacity and improved robustness when compared to traditional equilibrium-based SPME techniques, such as SPME fibers. Field measurements were performed in summer 2017 at the Station for Measuring Ecosystem-Atmosphere Relations (SMEAR II) in Hyytiälä, Finland. Complementary laboratory tests were also performed to compare the SPME-based techniques under controlled experimental conditions and to evaluate the effect of temperature and relative humidity on their extraction performance. The most abundant monoterpenes and aldehydes were successfully collected. A significant improvement on sampling capacity was observed with the new SPME Arrow system over SPME fibers, with collected amounts being approximately 2× higher for monoterpenes and 7-8× higher for aldehydes. BVOC species exhibited different affinities for the type of sorbent materials used (polydimethylsiloxane (PDMS)-carbon wide range (WR) vs. PDMS-divinylbenzene (DVB)). Higher extraction efficiencies were obtained with dynamic collection prior to equilibrium regime, but this benefit during the field measurements was small, probably due to the natural agitation provided by the wind. An increase in temperature and relative humidity caused a decrease in the amounts of analytes extracted under controlled experimental conditions, even though the effect was more significant for PDMS-carbon WR than for PDMS-DVB. Overall, results demonstrated the benefits and challenges of using SPME Arrow for the sampling of BVOCs in the atmosphere.

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“…To detect terpenes using SPME fibres, one of three sampling techniques is generally applied: (i) exposing fibres to the headspace of vials containing liquid or solid samples (Isidorov et al, 2003;Adam et al, 2005;Kos et al, 2006;Santos et al, 2006;Vichi et al, 2006), (ii) exposing fibres to air circulating over the sample (Zini et al, 2001;, or (iii) inserting fibres into a vial containing the sample . Because of the small dimensions of the sampling device and the simplicity and speed of the extraction procedure, HS-SPME is able to collect fragrances from live plants with minimum disturbance of the specimen, under both laboratory and field conditions.…”

Section: Headspace Methodsmentioning

confidence: 99%