Monitoring amyloid-β proteins aggregation based on label-free aptasensor

Zhang, Yuting; Figueroa-Miranda, Gabriela; Lyu, Zhaozi; Zafiu, Christian; Willbold, Dieter; Offenhäusser, Andreas; Mayer, Dirk

doi:10.1016/j.snb.2019.03.049

Cited by 64 publications

(39 citation statements)

References 51 publications

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“…Although device accuracy, selectivity, sensitivity, and response time are the major characteristics of DA sensors, the reusability of aptasensors can also become an advantage over immunosensors due to sustainability and waste prevention . Therefore, we tested the regeneration capability of our electrochemical biosensor.…”

Section: Resultsmentioning

confidence: 99%

A Novel Ratiometric Electrochemical Biosensor Based on a Split Aptamer for the Detection of Dopamine with Logic Gate Operations

Guo

Offenhäusser

et al. 2020

Physica Status Solidi (a)

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A novel dual‐signal ratiometric electrochemical biosensor based on a split aptamer is developed. A common shortcoming of amperometric aptamer sensors is unspecific signaling due to ssDNA conformational flexibility. Herein, a ratiometric detection using two competitive redox‐labeled aptamer strands is proposed. The neurotransmitter dopamine (DA) is chosen as a model target due to its importance for signal processing in the central nervous system. A DA aptamer is split into two parts, S1 and S2, where strand S1 is tethered to the electrode, whereas the second strand is labeled with methylene blue (MB) and acts as a redox reporter. Another ssDNA strand (CS1) tagged with anthraquinone (AQ) is introduced, which is complementary to strand S1 and reports on surface‐tethered strands that remain in its virgin state. In the presence of DA, CS1 is released from the surface due to the formation of S1–S2–DA complexes, resulting in decreasing AQ and increasing MB Faraday currents. Furthermore, logic gate operations can be performed to either improve signal reliability or enlarge the detection range. This proof‐of‐concept study demonstrates that the splitting of a full aptamer into two parts together with the use of complementary ratiomeric tests can improve the reliability of the sensor response.

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Section: Resultsmentioning

confidence: 99%

A Novel Ratiometric Electrochemical Biosensor Based on a Split Aptamer for the Detection of Dopamine with Logic Gate Operations

Guo

Offenhäusser

et al. 2020

Physica Status Solidi (a)

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“…The Aβ bonding with the aptamer was monitored by the ferro/ferricyanide redox probe. An ssDNA aptamer, receptor specific to AβO, was also self-assembled to the Au rod electrodes through Au–S interactions to construct EIS-based aptasensors for AβO assay [ 96 ]. A sandwich-type aptasensor based on metal–organic structures (MOFs) as signal probes has been developed for the detection of AβO [ 97 ].…”

Section: Electrochemical Aptasensors For Clinical Applicationmentioning

confidence: 99%

Electrochemical Aptasensors: Current Status and Future Perspectives

Abd-Ellatief

2021

Diagnostics

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This article reviews the progress of diversity of electrochemical aptasensor for target analytes detection. The immobilization strategies of aptamers on an electrode surface are addressed. The aptasensors are also introduced in compliance with the assay platforms. Many electrochemical aptasensors are nearly identical to conventional immunochemical approaches, sandwich and competition assays using electroactive signaling moieties. Others are “signal-on” and “sign-off” aptasensors credited to the target binding-induced conformational change of aptamers. Label-free aptasensors are also highlighted. Furthermore, the aptasensors applied for clinically important biomarkers are emphasized.

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“…The target can be small molecules, drugs, proteins, and cancer cells or viruses. Compared with traditional molecule recognition interactions like antibody–antigen binding, aptamers can provide many advantages such as simplicity of in vitro synthesis, easy labeling, high stability, and high sensitivity . Consequently, aptamers are still emerging for the development of biosensors because the conformational changes induced by target binding can be transduced into optical, electrochemical, and luminescent signal .…”

Section: Introductionmentioning

confidence: 99%

A Highly Sensitive Amperometric Aptamer Biosensor for Adenosine Triphosphate Detection on a 64 Channel Gold Multielectrode Array

Offenhäusser

Mayer

2020

Physica Status Solidi (a)

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Herein, an electrochemical aptamer biosensor based on a gold multielectrode array is reported for the detection of adenosine triphosphate (ATP), which is an important neurotransmitter in the central nervous system. The aptasensor possesses 64 individually addressable microelectrodes with a diameter of 24 μm, which is distributed over an area of 1 mm2. The electrodes are modified with an ATP‐specific aptamer, which is labeled with a ferrocene redox probe. It is demonstrated that the used chip fabrication and cleaning process generate microelectrode channels with similar electrochemical responses and electroactive surface areas. The immobilization of the aptamer on gold microelectrodes is optimized and the receptor density is determined to be (1.0 ± 0.44) × 1013 molecules cm−2. Concentration‐dependent alternating current voltammetry measurements exhibit a limit of detection of 0.30 × 10−9 m and a sensitivity of 2.4 nA logC. Furthermore, the sensor shows a high selectivity over analogous molecules and reproducible detection results in artificial cerebrospinal fluid. The sensor array is used to detect ATP via several channels of the same chip, showing high signal redundancy. A label‐free electrochemical, multichannel ATP aptasensor is demonstrated with high selectivity and subnanomolar sensitivity, which may be conducive to record this neurotransmitter in neuronal cell‐culture systems.

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Monitoring amyloid-β proteins aggregation based on label-free aptasensor

Cited by 64 publications

References 51 publications

A Novel Ratiometric Electrochemical Biosensor Based on a Split Aptamer for the Detection of Dopamine with Logic Gate Operations

A Novel Ratiometric Electrochemical Biosensor Based on a Split Aptamer for the Detection of Dopamine with Logic Gate Operations

Electrochemical Aptasensors: Current Status and Future Perspectives

A Highly Sensitive Amperometric Aptamer Biosensor for Adenosine Triphosphate Detection on a 64 Channel Gold Multielectrode Array

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