Molluscicidal activity of chlorophyll extraction against the freshwater snails

Effect of Chlorophyllin on Biomphalaria alexandrina Snails and Schistosoma mansoni Larvae

“…On the other hand, the limited effect on immature snails may be attributed to the great ability of juvenile snail's tissue to regenerate. These finding were in contrary with the findings of Mahmoud et al, (2013) who reported that chlorophyllin had resulted in 100% death of immature snails after three hours of sunlight exposure using concentrations up to 15µg/ml. While, the lethal effect on egg masses observed in the present study was also noticed by (Mahmoud et al, 2013) who reported 70% death of egg masses after three hours using concentrations up to 15µg/ml compared to 100% death of snails exposed to the same experimental conditions, denoting a lower effect on egg masses.…”

“…Where, chlorophyllin needed a minimum of four hours of sunlight exposure to exert its photodynamic action. These results came in accord with a previous study of (Mahmoud et al, 2013), who showed that the increased duration of sunlight exposure after chlorophyllin dark incubation was associated with higher mortality among Biomphalaria spp. snails.…”

“…Also, Wohllebe et al, (2012) found that Ichthyophthiriu smulftifiliis was killed using LC 50 of about 0.67 mg/L. A higher LC 50 was reported by (Mahmoud et al, 2013) who, noted that LC 50 was about 30 mg/L for the snails Lymnaeastagnalis, Biomphalaria spp. And Physamarmorata..…”

“…However, the used different preparations of chlorophyllingave different inaccurate lethal concentration (LC 50 ). (Mahmoud et al, 2013;Ragheb, 2013 andRagheb et al, 2013). Hence, it was important to use a standard chlorophyllin preparation and carry out the experiments under standard conditions to obtain the accurate LC 50 values.…”

“…(Salama et al, 2002;El-Tayeb, 2003;El-Tarky, 2005;Wohllebe, 2010 andRagheb, 2013) As human Schistosomiasis mansoni is still one of the major health problems in Egypt (Barakat, 2013 andLotfy, 2009). Using chlorophyllin in as a photosensitizer for control of the intermediate host, Biomphalaria snails by (Mahmoud et al, 2013;Ragheb, 2013;Ragheb et al, 2013). However, the used different preparations of chlorophyllingave different inaccurate lethal concentration (LC 50 ).…”