Molecularly imprinted uniform-size polymer-based stationary phase for high-performance liquid chromatography structural contribution of cross-linked polymer network on specific molecular recognition

Hosoya, Ken; Yoshizako, Kimihiro; Shirasu, Yuichi; Kimata, Kazuhiro; Araki, Takeo; Tanaka, Nobuo; Haginaka, Jun

doi:10.1016/0021-9673(95)01165-x

Cited by 115 publications

(56 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Uniformed spherical particles have been obtained by using multi-step swelling method [60][61][62][63]. Particles can be prepared directly in the form of spherical beads of controlled diameter.…”

Section: Multi-step Swelling Polymerizationmentioning

confidence: 99%

Characteristic and Synthetic Approach of Molecularly Imprinted Polymer

Yan

Row

2006

IJMS

448

231

View full text Add to dashboard Cite

Abstract:Molecularly imprinted polymers (MIP) exhibiting high selectivity and affinity to the predetermined molecule (template) are now seeing a fast growing research. However, optimization of the imprinted products is difficult due to the fact that there are many variables to consider, some or all of which can potentially impact upon the chemical, morphological and molecular recognition properties of the imprinted materials. This review present a summary of the principal synthetic considerations pertaining to good practice in the polymerization aspects of molecular imprinting, and is primarily aimed at researcher familiar with molecular imprinting methods but with little or no prior experience in polymer synthesis. The synthesis, characteristic, effect of molecular recognition and different preparation methods of MIP in recent few years are discussed in this review, unsolved problems and possible developments of MIP were also been briefly discussed.

show abstract

Section: Multi-step Swelling Polymerizationmentioning

confidence: 99%

Characteristic and Synthetic Approach of Molecularly Imprinted Polymer

Yan

Row

2006

IJMS

448

231

View full text Add to dashboard Cite

show abstract

“…Molecularly imprinted polymers (MIPs) have the ability to recognize target molecules specifically, and have been used in a variety of molecular recognition-based applications, such as chromatographic separation, [1][2][3][4][5] artificial antibody-mimics, 6 solid-phase exactions (SPE) [7][8][9] and biosensors. 10,11 The application that promises to be of greatest industrial significance in the immediate future is that of separation materials.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Uniform-size (-)-Ephedrine-imprinted Polymeric Microspheres by Multi-step Swelling and Suspension Polymerization

Zhang

et al. 2006

ANAL. SCI.

View full text Add to dashboard Cite

Ephedrine-imprinted polymeric microspheres have been prepared in an aqueous system by multi-step swelling and suspension polymerization, using methacrylic acid (MAA) as a functional monomer, and ethylene glycol dimethacrylate (EGDMA) as a cross-linker. Scanning electron microscopy (SEM) was used as a means to identify the structure features of the obtained polymers. Further, we examined the recognition mechanism of the polymers and the influences of some chromatographic conditions, such as the mobile-phase composition, flow-rate, column temperature and sample amount on the retentivity and selectivity for (-)-ephedrine and (+)-ephedrine. The results reveal that stable macroporous polymer beads with good size monodispersity were obtained, the average size of which was 3 -5 μm. Baseline chiral separation of the template isomers was achieved on a short column (50 mm × 4.6 mm i.d.) when the prepared polymer beads were used as a stationary phase, while the non-imprinted polymers (NIPs) did not show such ability. The optimized chromatographic condition was as follows: acetonitrile-acetic acid (99.8/0.2, v/v) as the mobile phase; sample amount, 40 -80 μg; flow rate, 1.0 ml min -1 ; and column temperature, room temperature, respectively. It is assumed that two classes of binding sites exist in the porous polymers, one being hydrophilic binding sites, the other being hydrophobic binding sites.

show abstract

“…14,15 The molar amounts of CN or CD, MAA and EDMA used for the preparation of MIPs were 1 mmol, 6 mmol and 25 mmol, respectively. The prepared polymers were packed into a stainless-steel column (4.6 mm i.d.…”

Section: Multi-step Swelling and Polymerization Methodsmentioning

confidence: 99%

Highly Stereoselective, Uniformly Sized Molecularly Imprinted Polymers for Cinchona Alkaloids in Hydro-Organic Mobile Phases

Haginaka

Kagawa

2003

ANAL. SCI.

Self Cite

View full text Add to dashboard Cite

Highly stereoselective, uniformly sized molecularly imprinted polymers (MIPs) for cinchona alkaloids, cinchonine (CN) and cinchonidine (CD), were prepared using methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EDMA) as a cross-linker. The MIPs were evaluated using a mixture of phosphate buffer and acetonitrile as the mobile phase. The CN-and CD-imprinted MAA-co-EDMA polymers can recognize the respective template molecule more than the other diastereomer, and afford an excellent diastereomer separation of CN and CD. In addition, the MIPs gave diastereomer separations of structurally related compounds, quinidine and quinine. The retentive and stereoselective properties of those compounds on the MIPs suggest that electrostatic and hydrophobic interactions can work to recognize these compounds. Furthermore, thermodynamic studies reveal that the entropy-driven effect is significant at mobile-phase pH 5.4, while the enthalpy-driven interactions seem to be dominant at mobile-phase pH 9.6.

show abstract

Molecularly imprinted uniform-size polymer-based stationary phase for high-performance liquid chromatography structural contribution of cross-linked polymer network on specific molecular recognition

Cited by 115 publications

References 19 publications

Characteristic and Synthetic Approach of Molecularly Imprinted Polymer

Characteristic and Synthetic Approach of Molecularly Imprinted Polymer

Preparation and Evaluation of Uniform-size (-)-Ephedrine-imprinted Polymeric Microspheres by Multi-step Swelling and Suspension Polymerization

Highly Stereoselective, Uniformly Sized Molecularly Imprinted Polymers for Cinchona Alkaloids in Hydro-Organic Mobile Phases

Contact Info

Product

Resources

About