Molecularly imprinted solid-phase extraction for the determination of ten macrolide drugs residues in animal muscles by liquid chromatography–tandem mass spectrometry

Abstract

A simple and sensitive method based on molecularly imprinted solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry was developed for the determination of the residues of ten macrolide drugs in swine, cattle and chicken muscles samples. The molecularly imprinted polymers (MIPs) were synthesized using tylosin as a template and methacrylic acid as a functional monomer. Samples were extracted with sodium borate buffer solution and ethyl acetate, and purified by the MIP cartridge. The results showed that the cartridge exhibited good recognition performance for macrolides, and better purification effect than the traditional solid-phase extraction cartridges. Recoveries of analytes at three spiking levels 1, 5 and 20μgkg(-1) ranged from 60.7% to 100.3% with the relative standard deviations less than 14%. The limits of detection of the method were between 0.1 and 0.4μgkg(-1). The method is useful for the routine monitoring of the residues of macrolide drugs in animal muscles.

Modern Extraction and Cleanup Methods of Veterinary Drug Residues in Food Samples of Animal Origin

“…Molecularly imprinted polymers (MIPs) are synthesized using a template, functional monomer, cross-linker and an initiator. MIPs are selective towards the target molecules, allowing them to be eluted from the SPE cartridge almost free of co-extracted compounds compared to classical sorbents used for clean-up procedures [56]. SPE sorbents such as C 18 , hydrophilic lipophilic balanced (HLB) material, diatomite, N-propylethylenediamine, alumina and Florisil are susceptible to interferences by impurities in biological samples and the cartridges can only be used once [57].…”

“…In a study conducted by Song et al [56], a MIP-SPE method combining LC-MS/ MS was developed to determine the residues of macrolide drugs in animal derived foods. Tylosin was used as a virtual template and the synthesized MIPs were used as the selective sorbent for packing SPE cartridge.…”

“…In high-fat foods like milk and meat, veterinary drug residues may bind to lipoproteins and extraction solvents forming emulsions and foam, especially polar veterinary drugs which may decrease recoveries and hence, affecting separation and analysis [56,62]. Extracting analytes from biological samples using modern extraction techniques like DLLME has some challenges.…”

Determination of macrolide antibiotics residues in pork using molecularly imprinted dispersive solid‐phase extraction coupled with LC–MS/MS

“…Therefore, a 2:3 ratio of chloroform to acetonitrile was chosen. Furthermore, the effect of different porogen volumes (10,15,20,25,30, and 35 mL) on adsorption was investigated ( Figure 2B). The adsorption capacity of MIP initially increases with the increasing porogen volume, but it gradually decreases with porogen volumes higher than 20 mL.…”

Preparation and characterization of molecularly imprinted polymers based on β‐cyclodextrin‐stabilized Pickering emulsion polymerization for selective recognition of erythromycin from river water and milk

“…Thus, it is important and urgent to develop approaches that are effective to remove the antibiotics from the environment. In different classes of antibiotics, macrolide class is the most commonly used in human [8] and veterinary medicine [9]. Erythromycin (ERY) is the first macrolide class antibiotics applied clinically to prevent infections, and it is effective against Gram-positive and few Gramnegative bacteria and is usually used to treat diseases that are caused by penicillin-resistant Staphylococcus aureus [10].…”