Molecularly imprinted polymer solid-phase extraction for detection of zearalenone in cereal sample extracts

Evaluation of two molecularly imprinted polymers for the solid‐phase extraction of natural, synthetic and mycoestrogens from environmental water samples before liquid chromatography with mass spectrometry

“…Chromatographic analysis was carried out by HPLC-MS and the recovery values obtained were higher than 90% in all cases. Concerning the use of the second cartridge (AFFINIMIP R SPE ZEN), only the work of Lucci et al [12] has been published. In this case, a single compound (ZEN) was extracted from cereal samples and analyzed by HPLC with fluorescence detection.…”

Current Trends in Sample Treatment Techniques for Environmental and Food Analysis

“…In both cases, very reliable baselines and similar recoveries were obtained, proving that the high selectivity of immunoaffinity sorbents also can be achieved with molecularly imprinted polymers SPE. Furthermore, previous studies have found MIP-SPE to have a similar selectivity but a higher capacity than commercial IAC columns [49,55]. These aspects are highly attractive for matrix clean-up, enrichment and selective extraction of analytes in difficult samples that are very common to food and environmental analyses.…”

“…Subsequent removal of the template leaves a cavity with specific recognition sites complementary in shape, size and functional groups to the target analyte (Figure 3). These recognition sites can specifically bind target compounds in a similar way that antibodies specifically bind to an antigen, with the advantages of being very selective without suffering from stability problems associated to biological receptors [49]. All these aspects, together with the fact that MIPs synthesis is also relatively easy and cheap when compared with the purification procedure of natural antibodies, have led to a considerable growth of interest in the use of MIPs in several analytical techniques and applications.…”

“…Under this reversedphase condition, the target analytes as well as non-polar interfering compounds are mainly retained by non-specific hydrophobic interactions. Thus, to generate specific interactions between the target compounds and the MIP and to disrupt the non-specific interactions between the residual monomers located at the surface of the polymer and matrix components, a selective washing step using low-to-medium polarity organic solvents, such as dichloromethane, chloroform, toluene or acetonitrile, is usually required [49]. It should be pointed out, however, that in some cases this selective washing step can be problematic because of the low polarity of common solvents used, which may give rise to miscibility problems and/or losses of the analyte [54].…”

Microextraction Techniques as a Sample Preparation Step for Metal Analysis

“…Main SPE methods for metal ions are summarized in Table 1 (Fritz, 1999). Increasing development of SPE during 1990s continues until recent years by introducing novel solid sorbents such as molecularly imprinted polymers (Lucci et al, 2010) and nanostructured materials (Faraji et al, 2010). SPE has many attractive features in comparison with classical solvent extraction methods.…”