Molecularly imprinted electrochemical aptasensor based on functionalized graphene and nitrogen-doped carbon quantum dots for trace cortisol assay

Yu, Chenhong; Li, Li; Ding, Yaping; Liu, Huajie; Cui, Hanyue

doi:10.1039/d1an01838h

Cited by 17 publications

(12 citation statements)

References 39 publications

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“…The sequence partially depends on the type of modification and the purification process that is tailored to the design of each sensor. Some of the cortisol aptamers used in the formation of electrochemical immunosensors are modified with thiols (-SH) at 5′ [ 42 , 44 , 45 , 46 ], and amines (-NH 2 ) and thiols at 5′ and 3′ [ 47 , 48 , 49 ], by the HPLC purification process. The immobilization process includes a cross-linker and physical adsorption through drop casting and incubation.…”

Section: Discussionmentioning

confidence: 99%

“…Klangphukhiew et al carried out the polymerization process by placing the pre-polymerization solution into a nitrogen furnace at a temperature of 70 °C for 24 h [ 55 ]. Incorporating nitrogen gas into the pre-polymerization process [ 9 , 48 , 56 , 57 , 58 ], or during the polymerization process [ 55 , 59 , 60 ], has been applied to the bulk imprinting method. It works as a degassing or purging agent to remove dissolved oxygen and eliminate compounds with low molecular weights that can potentially cause the constructed polymer to return to the monomer form [ 61 , 62 ].…”

Section: Molecularly Imprinted Polymermentioning

confidence: 99%

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Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

2022

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As a steroid hormone, cortisol has a close relationship with the stress response, and therefore, can be used as a biomarker for early detection of stress. An electrochemical immunosensor is one of the most widely used methods to detect cortisol, with antibodies as its bioreceptor. Apart from conventional laboratory-based methods, the trend for cortisol detection has seemed to be exploiting antibodies and aptamers. Both can provide satisfactory performance with high selectivity and sensitivity, but they still face issues with their short shelf life. Molecularly imprinted polymers (MIPs) have been widely used to detect macro- and micro-molecules by forming artificial antibodies as bioreceptors. MIPs are an alternative to natural antibodies, which despite demonstrating high selectivity and a low degree of cross-reactivity, often also show a high sensitivity to the environment, leading to their denaturation. MIPs can be prepared with convenient and relatively affordable fabrication processes. They also have high durability in ambient conditions, a long shelf life, and the ability to detect cortisol molecules at a concentration as low as 2 ag/mL. By collecting data from the past five years, this review summarizes the antibody and aptamer-based amperometric sensors as well as the latest developments exploiting MIPs rather than antibodies. Lastly, factors that can improve MIPs performance and are expected to be developed in the future are also explained.

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Section: Discussionmentioning

confidence: 99%

Section: Molecularly Imprinted Polymermentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

2022

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“…In addition, the recognition of other biomarkers such as cytochrome C [ 25 ], amyloid-β oligomers [ 31 ], dopamine [ 34 ], bovine serum albumin [ 56 ], prostate [ 57 ], scortisol [ 58 ] and thrombin [ 27 , 32 ] were reported based on MIP–aptamer dual recognition approach.…”

Section: Application Of Mip–aptamer In the Molecular Recognition Of C...mentioning

confidence: 99%

Molecularly Imprinting–Aptamer Techniques and Their Applications in Molecular Recognition

Zhou

Liu

2022

Biosensors

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Molecular imprinting–aptamer techniques exhibit the advantages of molecular imprinting and aptamer technology. Hybrids of molecularly imprinted polymer–aptamer (MIP–aptamer) prepared by this technique have higher stability, binding affinity and superior selectivity than conventional molecularly imprinted polymers or aptamers. In recent years, molecular imprinting–aptamer technologies have attracted considerable interest for the selective recognition of target molecules in complex sample matrices and have been used in molecular recognition such as antibiotics, proteins, viruses and pesticides. This review introduced the development of molecular imprinting–aptamer-combining technologies and summarized the mechanism of MIP–aptamer formation. Meanwhile, we discussed the challenges in preparing MIP–aptamer. Finally, we summarized the application of MIP–aptamer to the molecular recognition in disease diagnosis, environmental analysis, food safety and other fields.

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“…In addition to using a single biometric element to develop sensors, Huang et al (2021) presented a highly sensitive and selective electrochemical sensor with an aptamer-antibody sandwich mode A specific combination of antibodies and aptamers was utilized to identify the target cortisol. Whereafter, Yu et al (2022) suggested a novel electrochemical aptamer sensor using functionalized graphene (FG) and nitrogen-deqcarbon quantum dots (N-CQDs) integrating MIP techniques for trace analysis of cortisol in saliva samples.…”

Section: Electrochemical Sensor For the Detection Of Cortisolmentioning

confidence: 99%

Advances in electrochemical sensors based on nanomaterials for the detection of lipid hormone

Zhang

2022

Front. Bioeng. Biotechnol.

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Lipid hormone is produced by highly differentiated endocrine cells and directly secretes into the blood circulation or tissue fluid to act as information transmission. It influences the physiological functions of the human body by controlling the metabolic processes of multiple tissue cells. Monitoring the levels of lipid hormone is of great importance for maintaining human health. The electrochemical sensor is considered as an ideal tool to detect lipid hormone owing to its advantages such as quick response, convenience and low economic costs. In recent 3 years, researchers have developed various electrochemical sensors for the detection of lipid hormone to improve their sensitivity or selectivity. The use of nanomaterials (such as carbon nanomaterials, precious metal and polymer) is a key research object and a breakthrough for improving the sensing performance of electrochemical sensors for detection of lipid hormone. This paper reviews and discusses the basic principle, nanomaterials, actuality and future development trend of electrochemical sensors for the detection of lipid hormone in the past 3 years.

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Molecularly imprinted electrochemical aptasensor based on functionalized graphene and nitrogen-doped carbon quantum dots for trace cortisol assay

Abstract: This paper proposes a novel electrochemical aptasensor that integrates molecular imprinting techniques for trace analysis of cortisol. This sensor is based on functionalized graphene and nitrogen-doped carbon quantum dots. The...

Cited by 17 publications

References 39 publications

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol

Molecularly Imprinting–Aptamer Techniques and Their Applications in Molecular Recognition

Advances in electrochemical sensors based on nanomaterials for the detection of lipid hormone

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