Molecularly defined adult-type hypolactasia among working age people with reference to milk consumption and gastrointestinal symptoms

Anthoni, Sari

doi:10.3748/wjg.v13.i8.1230

Cited by 19 publications

(27 citation statements)

References 33 publications

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“…Data on previous diagnosis of atopy was also requested. The response rate was high as 99% of the participants returned the questionnaire [1]. All participants who reported milk-related symptoms (n 0756; 40% of those 1885 who filled in the questionnaire) were chosen for this study.…”

Section: Methodsmentioning

confidence: 99%

“…Milk consumption and milk-related abdominal symptoms were screened in a large population of 1900 adults in primary healthcare during spring 2004 [1]. All consecutive working-age patients in five primary care centres who had a referral for blood withdrawal in the laboratory were given the opportunity to join the study.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, the study group in this study comprised 857 adults (aged 18Á64 years) who were screened for food-specific IgE. In addition, the subjects had been genotyped for adult-type hypolactasia [1], and screened for coeliac disease [12]. The Pharmacia CAP System was used for screening of specific IgE against major food allergens (wheat, codfish, peanut, egg (ovalbumin), soybean, cow's milk).…”

Section: Methodsmentioning

confidence: 99%

“…The milk challenge was performed with low-lactose-containing milk to avoid symptoms related to lactose malabsorption. However, the subject who developed diarrhoea had the genotype C/C -13910 associated with low lactase level in the intestine (genotyped in our previous study on adult-type hypolactasia [1]). …”

Section: Milk Challengementioning

confidence: 99%

“…Recently, we reported that more than 40% of adults in primary care suspect they have experienced gastrointestinal symptoms after milk ingestion [1]. Among the possible aetiological factors for milk-related symptoms, adult-type hypolactasia is frequent in populations with high dairy intake, the prevalence ranging from 4% to 60% in Caucasian populations [2].…”

mentioning

confidence: 99%

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Should milk-specific IgE antibodies be measured in adults in primary care?

Anthoni

Elg

Haahtela

et al. 2008

Scandinavian Journal of Primary Health Care

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Milk Challengementioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Should milk-specific IgE antibodies be measured in adults in primary care?

Anthoni

Elg

Haahtela

et al. 2008

Scandinavian Journal of Primary Health Care

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Comparison of lactase persistence polymorphism in ancient and present‐day Hungarian populations

Nagy

Tömöry

Csányi

et al. 2011

American J Phys Anthropol

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The prevalence of adult-type hypolactasia varies ethnically and geographically among populations. A C/T-13910 single nucleotide polymorphism (SNP) upstream of the lactase gene is known to be associated with lactase non-persistence in Europeans. The aim of this study was to determine the prevalence of lactase persistent and non-persistent genotypes in current Hungarian-speaking populations and in ancient bone samples of classical conquerors and commoners from the 10th-11th centuries from the Carpathian basin; 181 present-day Hungarian, 65 present-day Sekler, and 23 ancient samples were successfully genotyped for the C/T-13910 SNP by the dCAPS PCR-RFLP method. Additional mitochondrial DNA testing was also carried out. In ancient Hungarians, the T-13910 allele was present only in 11% of the population, and exclusively in commoners of European mitochondrial haplogroups who may have been of pre-Hungarian indigenous ancestry. This is despite animal domestication and dairy products having been introduced into the Carpathian basin early in the Neolithic Age. This anomaly may be explained by the Hungarian use of fermented milk products, their greater consumption of ruminant meat than milk, cultural differences, or by their having other lactase-regulating genetic polymorphisms than C/T-13910. The low prevalence of lactase persistence provides additional information on the Asian origin of Hungarians. Present-day Hungarians have been assimilated with the surrounding European populations, since they do not differ significantly from the neighboring populations in their possession of mtDNA and C/T-13910 variants.

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Correlation of G/A -22018 single-nucleotide polymorphism with lactase activity and its usefulness in improving the diagnosis of adult-type hypolactasia among North Indian children

et al. 2012

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Adult-type hypolactasia (AtH or lactase non-persistence) is the physiological decline in lactase activity that manifests in majority of the world's population after weaning. Recently, various single-nucleotide polymorphisms (SNPs) upstream of lactase gene (LCT) have been suggested to be associated with AtH or the lactase persistent trait in different human populations. C/T -13910 SNP was found be completely associated with AtH in Finnish population, and G/A -22018 SNP was found to be strongly, but not completely, associated with AtH. The aim of this study was to correlate G/A -22018 SNP with intestinal lactase activity in North Indian children. These children were also genotyped for C/T -13910 SNP. We also examined the differences in milk consumption and milk-related clinical symptoms in children with different genotypes of G/A -22018 and C/T -13910 SNPs. Intestinal biopsies were obtained from 231 children aged 2-16 years undergoing routine endoscopy for various abdominal complaints. The biopsies were assayed for lactase, sucrase, and maltase activities and genotyped for G/A -22018 and C/T -13910 SNPs using restriction fragment length polymorphism and DNA sequencing analysis. There was a significant correlation between lactase activity and different genotypes of G/A -22018 SNP. Children with G/G -22018 genotype had low lactase activity. With a reference value of <10 U/g protein (lactase activity) to be indicative of AtH, the sensitivity and specificity of genetic test based on G/A -22018 SNP was 94.4 and 94.1 %, respectively. Furthermore, the consumption of milk was lower in children with G/G -22018 genotype. Flatulence was the only symptom significantly more frequent among the children with G/G -22018 genotype compared to those with G/A and A/A -22018 genotypes. However, most of the children with G/G -22018 genotype seem to tolerate small amounts of milk without any significant difference in gastrointestinal symptoms from those with G/A and A/A -22018 genotypes.

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Molecularly defined adult-type hypolactasia among working age people with reference to milk consumption and gastrointestinal symptoms

Abstract: AIM:To study milk consumption and subjective milkrelated symptoms in adults genotyped for adult-type hypolactasia.

Cited by 19 publications

References 33 publications

Should milk-specific IgE antibodies be measured in adults in primary care?

Should milk-specific IgE antibodies be measured in adults in primary care?

Comparison of lactase persistence polymorphism in ancient and present‐day Hungarian populations

Correlation of G/A -22018 single-nucleotide polymorphism with lactase activity and its usefulness in improving the diagnosis of adult-type hypolactasia among North Indian children

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