MolecularEpidemiology of orf513-Bearing Class 1 Integrons in MultiresistantClinical Isolates from ArgentineanHospitals

Arduino, S.; Catalano, Mariana; Orman, Betina; Roy, Paul H.; Centrón, Daniela

doi:10.1128/aac.47.12.3945-3949.2003

Cited by 35 publications

(29 citation statements)

References 21 publications

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“…It therefore seems likely that the associated ISCR1 element was involved in sequestering this section of DNA from the chromosome of K. ascorbata or a near relative into a (62), and in Morganella morganii (78). In addition, bla CTX-M2 has also been found in isolates of the gram-positive species Enterococcus faecium and serogroup G Streptococcus agalactiae (2), again in close association with ISCR1.…”

Section: Iscr1mentioning

confidence: 93%

“…The novel trimethoprim resistance gene dfrA23 was found on a nonconjugative plasmid, t-st4, of 110 kb in S. enterica serovar Typhimurium from Italy (104); dfrA18 was found on the incC group plasmid pDG0100 (93) and on a conjugative incF1 plasmid of 140 kb in S. enterica serovar Typhimurium from Albania (102); and dfrA10 was found in In34 (71) and as a component of two variants (SGI1A and SGI1D) of the Salmonella genomic island SGI1 on the chromosome of Salmonella enterica serovar Agona (see below) (15). The dfrA3b gene was found linked to ISCR1 in the C. freundii isolate from Argentina (2).…”

Section: Iscr1mentioning

confidence: 99%

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IS CR Elements: Novel Gene-Capturing Systems of the 21st Century?

Toleman

Bennett

Walsh

2006

Microbiol Mol Biol Rev

525

481

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SUMMARY “Common regions” (CRs), such as Orf513, are being increasingly linked to mega-antibiotic-resistant regions. While their overall nucleotide sequences show little identity to other mobile elements, amino acid alignments indicate that they possess the key motifs of IS91-like elements, which have been linked to the mobility ent plasmids in pathogenic Escherichia coli. Further inspection reveals that they possess an IS91-like origin of replication and termination sites (terIS), and therefore CRs probably transpose via a rolling-circle replication mechanism. Accordingly, in this review we have renamed CRs as ISCRs to give a more accurate reflection of their functional properties. The genetic context surrounding ISCRs indicates that they can procure 5′ sequences via misreading of the cognate terIS, i.e., “unchecked transposition.” Clinically, the most worrying aspect of ISCRs is that they are increasingly being linked with more potent examples of resistance, i.e., metallo-β-lactamases in Pseudomonas aeruginosa and co-trimoxazole resistance in Stenotrophomonas maltophilia. Furthermore, if ISCR elements do move via “unchecked RC transposition,” as has been speculated for ISCR1, then this mechanism provides antibiotic resistance genes with a highly mobile genetic vehicle that could greatly exceed the effects of previously reported mobile genetic mechanisms. It has been hypothesized that bacteria will surprise us by extending their “genetic construction kit” to procure and evince additional DNA and, therefore, antibiotic resistance genes. It appears that ISCR elements have now firmly established themselves within that regimen.

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Section: Iscr1mentioning

confidence: 93%

Section: Iscr1mentioning

confidence: 99%

IS CR Elements: Novel Gene-Capturing Systems of the 21st Century?

Toleman

Bennett

Walsh

2006

Microbiol Mol Biol Rev

525

481

View full text Add to dashboard Cite

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“…The combination of bla CTX-M-2 linked to ISCR1 is common in clinical isolates from South America (3,4,35,50), and this resistance gene encodes 69% of the extended-spectrum ␤-lactamases found in clinical isolates from Argentinean hospitals (3). Since a bla CTX-M-2 gene linked to ISCR1 was present in strain 12863, we designed primer pairs HS819/HS820, specific for ISCR1, and HS856/HS857, specific for bla CTX-M-2 (Table 1), and screened all 21 class 1-positive strains for their presence.…”

Section: Resultsmentioning

confidence: 99%

Urinary Tract Infections in a South American Population: Dynamic Spread of Class 1 Integrons and Multidrug Resistance by Homologous and Site-Specific Recombination

et al. 2008

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One hundred four bacterial strains mediating urinary tract infections in separate individuals from a Uruguayan community were isolated. Forty-six strains conferred a multidrug resistance phenotype. All 104 strains were examined for the presence of class 1, 2, and 3 integrons. Class 1 integrons were found in 21 isolates across four distinct bacterial genera. A large class 1 integron in a Klebsiella pneumoniae strain was fully sequenced and was 29,093 bp in length. This integron probably arose by homologous recombination since it was embedded in a hybrid Tn21-like transposon backbone which comprised a Tn5036-like tnp transposition module at the IRi integron end and a Tn21 mer module at the IRt integron end. The parent integron/transposon that contributed the Tn5036 module was not related to Tn1696 since the integron insertion points in the transposon backbones were 16 bases apart. Examination of the other 20 class 1 integron-containing strains revealed further evidence of genetic exchange. This included a strain that possessed a Tn5036 module at the IRt end but not at the IRi end and another that possessed a tnp module beyond IRi that was a hybrid of Tn21 and Tn5051 and that is presumed to have arisen by site-specific recombination. This study highlights the ability of different genetic elements to act cooperatively to spread and rearrange antibiotic resistance in a community.

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“…Almost all open reading frame (orf) 513-bearing class 1 integrons are associated with bla CTX-M-2 in the Gram-negative bacterial population under study and the sequences adjacent to the bla CTX-M-2 gene are conserved in all the studied isolates. 33) Eckert et al 34) examined the bla CTX-M genes in E. coli and K. pneumoniae by cloning, sequencing, and PCR analysis and detected a complex sul1-type integron including an orf513, that carried the bla gene together with its surrounding DNA. We found that 60% of intI1-positive isolates also contained the sul1 gene (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance, Class 1 Integrons and Extended-Spectrum .BETA.-Lactamases in Escherichia coli Clinical Isolates from Patients in South Thailand

Phongpaichit

Tunyapanit

Pruekprasert

2011

JOURNAL OF HEALTH SCIENCE

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Five hundred and ninety seven Escherichia coli (E. coli) isolates were obtained from clinical specimens at the Songklanagarind Hospital in Songkhla Province, Thailand during [2003][2004][2005]. Antimicrobial susceptibilities to ten antimicrobial agents were tested by a standard disk diffusion method. The presence of class 1 integrons was based on the detection of the integrase gene (intI1) by PCR. Extended-spectrum β-lactamases (ESBLs) were detected by a combination disk method. The highest percentage of resistance was found to ciprofloxacin (40.5%), norfloxacin (39.0%), and cefuroxime (33.2%). The IntI1 was detected in 59.5% of the tested isolates. Resistance to gentamicin, cefazolin, cefuroxime, cefotaxime, ceftriaxone, norfloxacin, and ciprofloxacin was significantly higher in class 1 integron-positive isolates (p < 0.05). The most prominent resistance pattern was for norfloxacinciprofloxacin (17.7%). ESBLs were detected in 75 out of 597 (12.6%) isolates; 56/302 (18.5%) and 19/295 (6.4%) were from hospitalized and non-hospitalized patients, respectively. Seventy-five percent of ESBL-positive strains were integron-positive isolates. Imipenem and meropenem were still able to inhibit all ESBL-producing strains. The results indicated that class 1 integrons are widely prevalent among clinical isolates of resistant E. coli especially in ESBL-producers and are probably a reservoir for producing multidrug resistance and nosocomial infections in hospitals.

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MolecularEpidemiology of orf513-Bearing Class 1 Integrons in MultiresistantClinical Isolates from ArgentineanHospitals

Cited by 35 publications

References 21 publications

IS CR Elements: Novel Gene-Capturing Systems of the 21st Century?

IS CR Elements: Novel Gene-Capturing Systems of the 21st Century?

Urinary Tract Infections in a South American Population: Dynamic Spread of Class 1 Integrons and Multidrug Resistance by Homologous and Site-Specific Recombination

Antimicrobial Resistance, Class 1 Integrons and Extended-Spectrum .BETA.-Lactamases in Escherichia coli Clinical Isolates from Patients in South Thailand

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