Antimicrobial Resistance, Class 1 Integrons and Extended-Spectrum .BETA.-Lactamases in Escherichia coli Clinical Isolates from Patients in South Thailand

Abstract

Five hundred and ninety seven Escherichia coli (E. coli) isolates were obtained from clinical specimens at the Songklanagarind Hospital in Songkhla Province, Thailand during [2003][2004][2005]. Antimicrobial susceptibilities to ten antimicrobial agents were tested by a standard disk diffusion method. The presence of class 1 integrons was based on the detection of the integrase gene (intI1) by PCR. Extended-spectrum β-lactamases (ESBLs) were detected by a combination disk method. The highest percentage of resistance was found to ciprofloxacin (40.5%), norfloxacin (39.0%), and cefuroxime (33.2%). The IntI1 was detected in 59.5% of the tested isolates. Resistance to gentamicin, cefazolin, cefuroxime, cefotaxime, ceftriaxone, norfloxacin, and ciprofloxacin was significantly higher in class 1 integron-positive isolates (p < 0.05). The most prominent resistance pattern was for norfloxacinciprofloxacin (17.7%). ESBLs were detected in 75 out of 597 (12.6%) isolates; 56/302 (18.5%) and 19/295 (6.4%) were from hospitalized and non-hospitalized patients, respectively. Seventy-five percent of ESBL-positive strains were integron-positive isolates. Imipenem and meropenem were still able to inhibit all ESBL-producing strains. The results indicated that class 1 integrons are widely prevalent among clinical isolates of resistant E. coli especially in ESBL-producers and are probably a reservoir for producing multidrug resistance and nosocomial infections in hospitals.

Antibiotics Resistance and Integron Class 1 among Commonsal Escherichia coli

“…Current results agreement with study of Phongpaichit et al, (2008) who found multiple drug resistance was more frequent in integron-positive isolates (89%) than those in integronnegative E. coli (57%) (2). Moreover study of Dureja and his coworker (2014) showed the class 1 integron positive isolates contain eight different resistance gene cassettes in five different combinations, namely dfrA12-orfF-aadA2, dfrA1-aadA1, dfrA17-aadA5, dfrA5 and dfrA7 and this similar to those observed earlier by Karczmarczyk et al (2011) that remember class 1 and class 2 integrons in the collection were found to contain trimethoprim (dfr) and streptomycin (aad) resistance-encoding genes, which are frequently reported in E. coli isolates recovered from various sources, including human, animal, and environmental samples (15,20).…”

Phenotypic and genotypic characterization of enteroaggregative Escherichia coli isolates from pediatric population in Pakistan

“…In Asian countries, the frequency of ESBL‐positive Enterobacteriaceae has been shown to be variable. National survey data have indicated the prevalence of ESBLs in 5–8% of E. coli isolates from Korea, Japan, Malaysia, and Singapore, but in 12–53% in Thailand, Taiwan, the Philippines, Indonesia, Hong Kong, China, and Iran .…”

Variability in gene cassette patterns of class 1 and 2 integrons associated with multi drug resistance patterns in Staphylococcus aureus clinical isolates in Tehran-Iran

“…Among different classes of integrons, class 1 integrons have been found more frequently in different species of bacteria, especially Gram-negative bacteria [ 11 , 26 , 27 ]. The classification of different integrons is mainly based on differences in the gene structure of integrases [ 11 ].…”