Molecular weight distribution of carrageenans by size exclusion chromatography and low angle laser light scattering

Lecacheux, D.; Panaras, R.; Brigand, G.; Martin, George C.

doi:10.1016/0144-8617(85)90003-7

Cited by 62 publications

(37 citation statements)

References 25 publications

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“…Typical molar mass values for k-carrageenan in 0.1M NaCl reported in the literature range from about 300,000 to 430,000 g/mol. 10,15,16 Using flow FFF-MALS, we obtained a value of 68 nm for the radius of gyration, in good correspondence with results obtained from Zimm plot analyses, 9,10 but significantly larger than obtained in SEC-MALS experiments (48 nm). 9 The hydrodynamic radius at the peak maximum, ible polymers in good solvents (Table I).…”

Section: Resultssupporting

confidence: 86%

Conformational change and aggregation of κ-carrageenan studied by flow field-flow fractionation and multiangle light scattering

et al. 1998

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The relatively novel combination of flow field‐flow fractionation (FFF) and multiangle light scattering (MALS) was employed to study a nondegraded κ‐carrageenan in different 0.1M salt solutions. The applicability of the technique was tested, and the effects of salt type and salt composition on the molar mass and radius of gyration were studied. A conformational ordering was induced at room temperature by switching the solvent from 0.1M NaCl (coil form) to 0.1M NaI (helix form). An approximate doubling of the average molar mass and an increase in radius of gyration was then observed, in agreement with results obtained previously using size exclusion chromatography–MALS. This increase in size was attributed to conformational ordering and to the formation of double helices. Severe aggregation was observed above 40% CsI in the 0.1M mixed salt solution of CsI and NaI. This was ascribed to the association of helices into large aggregates. For these large associates, having molar masses of several millions, a reversal of the elution order in flow FFF was detected. © 1998 John Wiley & Sons, Inc. Biopoly 45: 85–96 1998

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Section: Resultssupporting

confidence: 86%

Conformational change and aggregation of κ-carrageenan studied by flow field-flow fractionation and multiangle light scattering

et al. 1998

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“…21 Commercial carrageenans have very broad molecular weight distributions, with a characteristic low molecular weight "tail." 22,23 The effect of polydispersity on the conformational properties of carrageenans appears to be unexplored.…”

Section: 8 -12mentioning

confidence: 99%

“…In addition, the removal of low-M w species facilitates the determination of the number average molecular weights (M n ) by SEC-LALLS, which previously has been reported with large uncertanties. 22,23 The conformational properties of the carrageenans are defined on the basis of optical rotation at the same conditions as in the SEC-LALLS experiments. In order to observe a possible concentration-dependent conformational transition, we reinvestigate the specific optical rotation in the ordered states at very low concentrations.…”

Section: 8 -12mentioning

confidence: 99%

Analysis of the conformational properties of ?- and ?-carrageenan by size-exclusion chromatography combined with low-angle laser light scattering

1999

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“…Carrageenans are sulfated linear polysaccharides of alternating 1,3-linked b-D-galactopyranose and a-1,4-linked a-1,4-linked a-D-galactopyranose, extracted from various red seaweeds of the family Rhodophyceae [1]. Their molecular masses range between 300 and 600 kDa [2]. The differences between carrageenans are due to the position and the number of sulfate groups and the presence of the anhydro bridge as shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

Separation of ι-, κ and λ-carrageenans by capillary electrophoresis

2001

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The present study reports a novel method for the separation of the high-molecular-weight anionic polysaccharides, iota, kappa, and lambda carrageenans, in capillary electrophoresis (CE). Carrageenan samples are first derivatised with 9-aminopyrene-1,4,6-trisulfonic acid (APTS), separated in an ammonium acetate background electrolyte (BGE) and detected with laser-induced fluorescence (LIF). The effects of changes of instrumental parameters (temperature, injection mode, field strength) and the composition of the BGE (concentration and pH) are reported, and are explained in terms of the physical chemistry of the BGE and the biopolymers. Optimal separation conditions for kappa, iota, and lambda carrageenans, including an APTS internal standard, were found in a polyvinyl alcohol coated capillary with an ammonium acetate BGE of low concentration (25 mM) and moderate pH (8.0). This BGE gave the best reproducibility in tests on iota/kappa mixtures, with relative standard deviations (RSDs) in migration times and normalised peak areas (relative to the APTS internal standard) of less than 0.1% and 1%, respectively. Using this BGE at 50 degrees C and a voltage of 30 kV, all three carrageenan subtypes were separated in a run time of 3 min.

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Molecular weight distribution of carrageenans by size exclusion chromatography and low angle laser light scattering

Cited by 62 publications

References 25 publications

Conformational change and aggregation of κ-carrageenan studied by flow field-flow fractionation and multiangle light scattering

Conformational change and aggregation of κ-carrageenan studied by flow field-flow fractionation and multiangle light scattering

Analysis of the conformational properties of ?- and ?-carrageenan by size-exclusion chromatography combined with low-angle laser light scattering

Separation of ι-, κ and λ-carrageenans by capillary electrophoresis

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