Analysis of the conformational properties of ?- and ?-carrageenan by size-exclusion chromatography combined with low-angle laser light scattering

Hjerde, Torgeir; Smidsrød, Olav; Christensen, Bjørn E.

doi:10.1002/(sici)1097-0282(199901)49:1<71::aid-bip7>3.0.co;2-h

Cited by 46 publications

(29 citation statements)

References 27 publications

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“…Such deviations from the ideal formulae are consistent with ∼ 11% iota‐segments in the applied kappa‐carrageenan or 7% kappa‐segments in the iota‐carrageenan. Almost the same composition was found by H NMR11, 12 or NMR/IR14 experiments on Sigma fine chemicals prior to and after purification.…”

Section: Resultssupporting

confidence: 93%

Physicochemical characterization of carrageenans—A critical reinvestigation

Berth¹,

Vuković

Lechner

2008

J of Applied Polymer Sci

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Kappa-, iota-, and lambda-carrageenan (food grade) were analyzed by static light scattering (MALS in batch mode) in 0.1M NaNO 3 at 25 and 60 C, earlier heated up to 90 C or not. At 25 C, there was a strong tendency for a concentration-dependent aggregation in the order lambda < kappa < iota. At 60 C, all samples were molecularly dispersed. The strongly temperature-dependent refractive index increments (equilibrium dialysis) differ. Data interpretation in terms of the wormlike chain model using the Skolnik-Odijk-Fixman approach led to an intrinsic persistence length around 3 to 4 nm and expansion factors as high as 1.5 and above in a thermodynamically good solvent for all three types. and a nearly identical molar mass dependence of the radius of gyration. HPSEC at 25 C on kappa-carrageenan confirmed formation of soluble aggregates. Special emphasis was put on analytical and methodological aspects. The reliability of the experimental data was demonstrated by analogous measurements on dextran calibration standards.

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Section: Resultssupporting

confidence: 93%

Physicochemical characterization of carrageenans—A critical reinvestigation

Berth¹,

Vuković

Lechner

2008

J of Applied Polymer Sci

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“…This has been achieved by operating both column and eluent at high temperature and by adding iodide salts of non-gelling cations [e.g., Li + , Na + , N(CH 3 ) 4 + , etc.] to the solvent, since these salts induce an ordered conformation without further aggregation (Hjerde, Smidsrød, & Christensen, 1999;Sworn, Marrs, & Hart, 1987).…”

Section: Molecular Weight Analysis Through Size-exclusion Chromatogramentioning

confidence: 99%

“…They comprise refractive index (RI) (Ekeberg, Knutsen, & Sletmoen, 2001;Ekström & Kuivinen, 1983;Karlsson & Singhb, 1999;Knutsen, Moe, Larsen, & Grasdalen, 1993;Knutsen et al, 2001;Montolalu, Tashiro, Matsukawa, & Ogawa, 2007;Spichtig & Austin, 2008;Sworn et al, 1987), low-angle laser light scattering (LALLS) (Hjerde et al, 1999), multi-angle light scattering (MALS) (Girod, Baldet-Dupy, Maillols, & Devoisselle, 2001) and vacuumultraviolet inductively coupled plasma-atomic emission spectrometry (ICP) (Uno et al 2001). Triple detector system (RI-viscometerlight scattering) (Myslabodski, Stancioffh, & Heckertta, 1996) and dual detector systems: RI and LALLS (Slootmaekers et al, 1991), RI and light scattering (Singh & Jacobsson, 1994;Viebke, Borgström, & Piculell, 1995), RI and MALS (Marcelo, Saiz, & Tarazona, 2005) have also been described.…”

Section: Molecular Weight Analysis Through Size-exclusion Chromatogramentioning

confidence: 99%

Carrageenans: Biological properties, chemical modifications and structural analysis – A review

Campo

Kawano

Silva

et al. 2009

Carbohydrate Polymers

1,099

558

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“…Several physicochemical studies reveal the existence of iota-carrageenan doublets, i.e. two chains together even at dilute concentrations (Hjerde, Smidsrod & Christensen, 1999; McIntire & Brant, 1999; Viebke, Borgstrom & Piculell, 1995). X-ray studies confirm that these two chains are intertwined and held strongly by O-6H⋯O-2 hydrogen bonds and ordered helical arrangements are present in the junction zones.…”

Section: Discussionmentioning

confidence: 99%

Impact of urea on the three-dimensional structure, viscoelastic and thermal behavior of iota-carrageenan

Patel

Campanella

Janaswamy

2013

Carbohydrate Polymers

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Urea breaks hydrogen bonds among biopolymers leading to structural destabilization. In the case of hydrocolloids urea addition is thought to impact gelation. Detailed information about its pertinent role on influencing the structure-function relationships of hydrocolloids is still elusive, however. The present investigation is aimed at delineating hydrocolloids structural behavior in the presence of urea employing iota-carrageenan as a model system. X-ray fiber diffraction, rheological and thermal properties of two iota-carrageenan solutions with weight concentrations 4.5 and 6.0% w/w at two urea molar concentrations (0.5 and 2.0 M) with and without heat treatments have been analyzed. X-ray results suggest that the canonical double helical structural arrangement of iota-carrageenan is maintained even after urea addition. However, improved crystallinity, ordering and altered unit cell dimensions especially with heat treatments of the binary mixtures indicate the promotion of favorable interactions among carrageenan helices in the presence of urea. Increased elastic modulus and onset temperature of melting endotherm with the heat treatment compared to cold addition further attests the X-ray observations of enhanced structural ordering. Overall, results suggest that urea molecules synergistically aid iota-carrageenan interactions and stabilize structure of junction zones. Our findings are deemed to be helpful in the design and development of novel non-food applications of hydrocolloids.

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Analysis of the conformational properties of ?- and ?-carrageenan by size-exclusion chromatography combined with low-angle laser light scattering

Cited by 46 publications

References 27 publications

Physicochemical characterization of carrageenans—A critical reinvestigation

Physicochemical characterization of carrageenans—A critical reinvestigation

Carrageenans: Biological properties, chemical modifications and structural analysis – A review

Impact of urea on the three-dimensional structure, viscoelastic and thermal behavior of iota-carrageenan

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