Molecular weight analysis of water-soluble poly(phenylene ethynylene)s using MALDI-TOF MS

Wu, Jiatao; Tan, Chunyan; Zhou, Xuan‐Wei; Tan, Ying; Yang, Pengyuan; Jiang, Yuyang

doi:10.1002/pola.28650

Cited by 7 publications

(5 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, dissociated counterions dominate in polyelectrolyte solutions without salt and give a much larger Π than neutral polymer solutions at the same polymer concentration . Other methods such as NMR end-group analysis and matrix-assisted laser desorption/ionization mass spectrometry are limited to low-molecular-weight polymers (<25 kg/mol) and hence are not ideal methods. − Thus, new techniques for determining the molecular weights of polyelectrolytes are necessary to further advance their fundamental understanding as well as enable rapid development of new polyelectrolyte chemistries and their related technologies. − …”

Section: Introductionmentioning

confidence: 99%

Determining the Molecular Weight of Polyelectrolytes Using the Rouse Scaling Theory for Salt-Free Semidilute Unentangled Solutions

et al. 2022

View full text Add to dashboard Cite

Chain dynamics in the semidilute unentangled regime can be used to determine the molecular weight of polyelectrolytes based on the Rouse scaling model. Four methods enable determination of the number density of chains via measurements of correlation length (ξ) by small-angle X-ray scattering, specific viscosity (η sp ) and relaxation time (τ) by rheometry, and diffusion coefficient (D) by NMR. Five narrow dispersity cesium polystyrene sulfonate (CsPSS) solutions without salt are studied in water, anhydrous ethylene glycol, and anhydrous glycerol to test all methods. Combining viscosity and correlation length yields the weightaverage molecular weight (M w ) from the Rouse model. Combining diffusion coefficient and correlation length in water provides number-average molecular weight (M n ) reliably for chains with N < 2000 repeat units. Glycerol slows relaxation dynamics, and the shear rate dependence of viscosity yields reliable τ for CsPSS with N > 100, which is governed by the product of z-average and z + 1 average molecular weight (M z M z+1 ) in the Rouse model. Terminal modulus G = (η − η s )/τ via rheometry correlates with M w /M z M z+1 .

show abstract

Section: Introductionmentioning

confidence: 99%

Determining the Molecular Weight of Polyelectrolytes Using the Rouse Scaling Theory for Salt-Free Semidilute Unentangled Solutions

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Matrix assisted laser desorption ionization (MALDI) is an ionization technique that has shown wide use in the analysis of polymers, [2][3][4] including acrylates, 5 and polymers prepared by living radical polymerization. [6][7][8][9][10][11][12][13][14][15][16] MALDI-ToF has been utilized to give values for the molecular weight, 17,18 information on polymer end groups, [19][20][21][22][23][24] as well as to probe the mechanisms of polymer synthesis. [25][26][27][28] It is a soft ionization technique where fragmentation and double charged species are rare, 29,30 allowing for good ionization of low to medium weight nonvolatile compounds.…”

Section: Introductionmentioning

confidence: 99%

MALDI-LID-ToF/ToF analysis of statistical and diblock polyacrylate copolymers

2018

View full text Add to dashboard Cite

show abstract

“…The detection of polymer peaks on MALDI‐TOF followed the protocol for cationic polymers using volume ratios of polymer to matrix of 1:10 at polymer concentration of 5 mg mL −1 in MQ and matrix concentration of 10 mg mL −1 . [ 48 ] In our case the presence of cationic agent was insignificant. The matrix used was 2,5‐dihydroxybenzoic acid (DHB) prepared in 0.1% TFA.…”

Section: Methodsmentioning

confidence: 55%

Reversible Protection and Targeted Delivery of DNA Origami with a Disulfide‐Containing Cationic Polymer

Youssef,

Tsang,

Samanta

et al. 2023

Small

View full text Add to dashboard Cite

DNA nanostructures have considerable biomedical potential as intracellular delivery vehicles as they are highly homogeneous and can be functionalized with high spatial resolution. However, challenges like instability under physiological conditions, limited cellular uptake, and lysosomal degradation limit their use. This paper presents a bio‐reducible, cationic polymer poly(cystaminebisacrylamide‐1,6‐diaminohexane) (PCD) as a reversible DNA origami protector. PCD displays a stronger DNA affinity than other cationic polymers. DNA nanostructures with PCD protection are shielded from low salt conditions and DNase I degradation and show a 40‐fold increase in cell‐association when linked to targeting antibodies. Confocal microscopy reveals a potential secondary cell uptake mechanism, directly delivering the nanostructures to the cytoplasm. Additionally, PCD can be removed by cleaving its backbone disulfides using the intracellular reductant, glutathione. Finally, the application of these constructs is demonstrated for targeted delivery of a cytotoxic agent to cancer cells, which efficiently decreases their viability. The PCD protective agent that is reported here is a simple and efficient method for the stabilization of DNA origami structures. With the ability to deprotect the DNA nanostructures upon entry of the intracellular space, the possibility for the use of DNA origami in pharmaceutical applications is enhanced.

show abstract

Molecular weight analysis of water-soluble poly(phenylene ethynylene)s using MALDI-TOF MS

Cited by 7 publications

References 43 publications

Determining the Molecular Weight of Polyelectrolytes Using the Rouse Scaling Theory for Salt-Free Semidilute Unentangled Solutions

Determining the Molecular Weight of Polyelectrolytes Using the Rouse Scaling Theory for Salt-Free Semidilute Unentangled Solutions

MALDI-LID-ToF/ToF analysis of statistical and diblock polyacrylate copolymers

Reversible Protection and Targeted Delivery of DNA Origami with a Disulfide‐Containing Cationic Polymer

Contact Info

Product

Resources

About