Molecular view of the structural reorganization of water in DPPC multilamellar membranes induced by l -cysteine methyl ester

Arias, Juan Marcelo; Tuttolomondo, M.E.; Díaz, Sergio; Altabef, A. Ben

doi:10.1016/j.molstruc.2017.11.124

Cited by 10 publications

(12 citation statements)

References 30 publications

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“…In addition, the wavenumbers of the ν s CH 2 and ν a CH 2 bands also reflect conformational order and interchain coupling (generally, wavenumber shifts toward higher values mean an increase in the decoupling of the chains). These results show that there is no increase in the intensity ratio at the molar ratios studied. ,,, …”

Section: Results and Discussionmentioning

confidence: 61%

“…These results show that there is no increase in the intensity ratio at the molar ratios studied. 19,20,26,33 The δCH 2 and τCH 2 bands corresponding to Cys/DPPC are shifted toward lower frequencies with respect to the pure DPPC in the Raman spectrum for all molar ratios (Table S4 and Figure 5). The frequency of these modes has also been associated with the degree of coupling between alkane chains, whereas the decoupling has been correlated with a frequency increase of this mode.…”

Section: Resultsmentioning

confidence: 96%

“…19,46 An intense band corresponding to the C−S stretching mode of the Cys water solution at 681 cm −1 is observed for all molar ratios in the gel state in the 800−600 cm −1 region. 19,20,46 The interaction of water with the choline groups (N−(CH 3 ) 3 and C−N) has been studied with different phospholipids in the literature. The peaks observed at 3042 and 720 cm −1 represent the stretching vibration of the N−(CH 3 ) 3 and C−N bond of the DPPC choline group in the gel state (Figure 9).…”

Section: Resultsmentioning

confidence: 99%

“…The water structures in the head group region and water−hydrocarbon interface can be specific for different types of biomolecules (drugs) or different aminoacids in peptides that make water like a sensor to trigger the response of the biomembranes to external perturbations. 19,20 We studied the Cys/DPPC interaction to analyze how Cys modifies the different phases of the lipids. Studying the specific interaction with the groups of the hydrophilic and hydrophobic regions of lipid biology and taking into account that the changes in the organization of water in the lipid interface could affect the activity or formation of some proteins, such as glycoproteins secreted by goblet cells.…”

Section: Introductionmentioning

confidence: 99%

“…This interaction modifies the degree of hydration of the lipid bilayer by altering some properties of membranes, such as the transition temperature, permeability, and fluidity. The water structures in the head group region and water–hydrocarbon interface can be specific for different types of biomolecules (drugs) or different aminoacids in peptides that make water like a sensor to trigger the response of the biomembranes to external perturbations. , …”

Section: Introductionmentioning

confidence: 99%

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Reorganization of Hydration Water of DPPC Multilamellar Vesicles Induced by l-Cysteine Interaction

et al. 2018

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The aim of this study is to analyze the consequences of water redistribution on the structure and stability of phospholipid bilayers induced by cysteine (Cys). This interaction is studied with 1,2-dipalmitoyl- sn-glycero-3-phosphatidylcholine (DPPC) multilamellar vesicles in gel (30 °C) and liquid crystalline (50 °C) state; experimental studies were performed by means of Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, and differential scanning calorimetry (DSC). The polar head sites of the lipid molecules to which water can bind are identified by competition with compounds that form hydrogen bonds, such as Cys. FTIR spectroscopy results revealed that there is a Cys interaction with the phospholipid head groups in the gel and liquid crystalline phases. Raman spectra were measured in the gel state. They were dominated by vibrations of the fatty acyl chains, with superposition of a few bands from the head group, and clearly showed that the S-H stretching band of Cys shifted to lower frequencies with a decrease in its force constant. DSC disclosed an overview of the behavior of the multilamellar vesicles in the working temperature range (30-50 °C) and showed how the increase of the molar ratios modified the environment of the polar head and the hydrocarbon chains. A loss of the pretransition ( T) and an increase in the temperature of main transition ( T) with increasing Cys/DPPC molar ratio were observed.

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