Molecular variation of type 1 vaccine-related and wild polioviruses during replication in humans

Characterization of Strains of Type 3 Poliovirus by Oligonucleotide Mapping

“…This conclusion is entirely consistent with data reported for other viruses, including influenza (Nakajima et al, 1978), VSV (Clewley et al, 1977), mouse hepatitis virus (Lai & Stohlman, 1981), FMDV (Robson et al, 1979, and type 1 and type 2 poliovirus (Nottay et aL, 1981). For FMDV, as for poliovirus, not only are the oligonucleotide maps of different serotypes totally distinct, but the maps of strains within a serotype also bear no obvious relation to each other (Robson et al, 1979Nottay et al, 1981;Minor, 1980). The high degree of variation can be predicted from the assumptions that the characteristic oligonucleotide map is made from up to approx.…”

“…In contrast, the maps of strains which are related tend to be similar for the range of viruses for which published data are available Nottay et al, 1981;Clewley et al, 1977;Young et al, 1979), although there are frequently changes in a few spots. The data reported here and previously (Minor, 1980) indicate that this is also true for type 3 poliovirus.…”

“…For FMDV, different strains of the same serotype can appear to be completely unrelated by T1 oligonucleotide mapping, while showing considerable homology in hybridization experiments (Robson et al, 1979 or by limited nucleic acid sequencing (Harris et al, 1980). Similar variation in the maps of different strains for type 1 and type 2 poliovirus have been reported (Nottay et al, 1981), and also for a limited number of type 3 poliovirus strains (Minor, 1980). Strains which generate similar oligonucleotide maps are, therefore, likely to be extremely closely related.…”

“…This has been accomplished by T1 RNase oligonucleotide mapping for many viruses, including vesicular stomatitis virus (VSV) (Clewley et al, 1977), influenza virus (Nakajima et al, 1978), mouse hepatitis virus (Lai & Stohlman, 1981), foot-and-mouth disease virus (FMDV) (Robson et aL, 1979Harris et al, 1980) and poliovirus (Minor, 1980;Nottay et al, 1981). In all cases it has proved highly sensitive to strain variation.…”

The molecular epidemiology of type 1 poliovirus in Central African Republic.

“…constant rate on passage through humans (Nottay et al, 1981). Genotypes of polioviruses have been defined as groups of strains that show more than 85 % sequence identity in the VP1\2A region (Rico-Hesse et al, 1987).…”