Molecular variation in <i>Leymus</i> species and populations

Anamthawat‐Jónsson, Kesara; Bragason, Birkir Thor; Bödvarsdóttir, Sigrídur K.; Koebner, And R. M. D.

doi:10.1046/j.1365-294x.1999.00575.x

Cited by 15 publications

(5 citation statements)

References 16 publications

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“…The section Leymus consists mainly of three morphologically similar species: the octoploid type species L. arenarius and two tetraploid species L. racemosus and L. mollis (L öve 1984). Leymus arenarius has three major rDNA loci identical to those of L. racemosus , supporting the molecular evidence that the northern European L. arenarius probably derived from the Eurasian L. racemosus via allopolyploidy (A namthawat ‐J ónsson et al 1999; A namthawat ‐J ónsson and B ödvarsdóttir 2001). The American L. mollis is genetically different from its Eurasian counterparts, although they are morphologically similar.…”

Section: Discussionsupporting

confidence: 65%

Identifying the genome of wood barley Hordelymus europaeus (Poaceae: Triticeae)

et al. 2006

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Wood barley, Hordelymus europaeus, was compared with other Triticeae species by Southern and fluorescence in situ hybridisation using total genomic DNA and repetitive sequences as probes. On Southern blots, the total genomic probe from H. europaeus hybridised strongly to DNA of its own species and to Leymus and Psathyrostachys, indicating the presence of Ns genome in H. europaeus. Furthermore, the total genomic probe from P. fragilis hybridised to DNA of H. europaeus as much as to all of the Psathyrostachys and Leymus species examined. Ns genome-specific DNA sequences isolated from L. mollis (pLmIs1, pLmIs44 and pLmIs53) hybridised essentially to H. europaeus and all of the species of Leymus and Psathyrostachys. Chromosomal localization of these clones on H. europaeus confirmed the presence of Ns genome-specific DNA on all chromosomes, indiscriminately. Under moderate hybridisation stringency the Ns genome-specific probes, together with repetitive sequences pTa71 and pAesKB7, produced species-specific RFLP banding profiles on Southern blots. A phenetic tree based on these profiles revealed a distinct Ns species cluster within the Triticeae, represented by Leymus and Psathyrostachys species. Hordelymus europaeus belonged to this Ns cluster. Chromosomal mapping of the 18S-25S and the 5S ribosomal genes, together with the repetitive sequence pLrTaiI, corroborated that H. europaeus was most probably related to Leymus, especially the European/Eurasian members of sect. Leymus. In an attempt to identify the genome of H. europaeus, different approaches were employed; the results clearly showed that wood barley had the Ns basic genome and nothing else.

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Section: Discussionsupporting

confidence: 65%

Identifying the genome of wood barley Hordelymus europaeus (Poaceae: Triticeae)

et al. 2006

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“…The fact that these 2 repetitive sequences could vary from total absence to a high number of sites in the genome of Leymus species suggests that they are neither chromosome nor genome specific. Variation in AFLP profiles between old and new populations of an Alaskan Leymus mollis accession in Iceland was attributed to genotype changes resulting from changes in environment or ecosystem and migration and colonization of the species in a new environment, i.e., from Alaska to southern Iceland (Anamthawat-Jónsson et al 1999). Similarly, variation in the abundance of repetitive se- quences observed in this study might also be attributed to genotypic differences.…”

Section: Sts-pcrsupporting

confidence: 51%

“…Variation in repetitive sequences has been used for studying germplasm diversity and relationships in Amaranthus (Sun et al 1999). Variation in amplified fragment length polymorphism (AFLP) and restriction fragment length polymorphism (RFLP) of ribosomal genes has been observed between Leymus species and among populations of Leymus arenarius (L.) Hochst., and Leymus mollis (Anamthawat-Jónsson et al 1999). In this paper, we surveyed the variation in the abundance of pLrTaiI-1 and pLrPstI-1 among accessions and species of Psathyrostachys and Leymus.…”

Section: Introductionmentioning

confidence: 99%

Variations in abundance of 2 repetitive sequences in Leymus and Psathyrostachys species

Wang¹,

Zhang²,

Lee³

et al. 2006

Genome

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The Ns genome of the genus Psathyrostachys is a component of the polyploid genome in the genus Leymus. Using fluorescence in situ hybridization (FISH), the occurrence and abundance of 2 tandem repetitive sequences from Leymus racemosus (Lam.) Tzvelev, pLrTaiI-1 (TaiI family) and pLrPstI-1 (1 class of 350-bp family), were assayed in 4 species of the genera Psathyrostachys and Leymus. The pLrPstI-1 sequence was absent in all 4 Psathyrostachys species. While P. fragilis and P. huashanica did not have the pLrTaiI-1 sequence, 15 accessions of P. juncea and 2 accessions of P. lanuginosa had pLrTaiI-1 sites ranging in number from 7 to 16 and from 2 to 21, respectively. The numbers of pLrTaiI-1 and pLrPstI-1 sites were 1-24 and 0-30, respectively, in L. ramosus; 2-31 and 5-36 in L. racemosus; 0-4 and 0 in L. mollis; 2-9 and 24-27 in L. secalinus. The FISH assay on pLrTaiI-1 was successfully converted to a sequence-tagged-site polymerase chain reaction (STS-PCR) test using a primer pair designed from the sequence of this repetitive DNA. Seventy-three accessions representing 27 Leymus species were assayed for the abundance of pLrTaiI-1 by STS-PCR. With a few exceptions of uniformity in some accessions, nearly all Leymus species observed were heterogeneous for the abundance of pLrTaiI-1 sequence and no Leymus species was totally devoid of this repetitive sequence. These findings may have significance for the understanding of phylogeny, nature of polyploidy, adaptive ranges, and breeding potential of Leymus species.

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“…some variation in leymin seed storage protein among L. urenarius populations from southern Finland (AHOKAS 1992), we chose a genome-wide method of amplified fragment length polymorphism (AFLP) for our materials and followed the protocol of DON IN^ et al (1997). Generation of AFLP fingerprints for Leynius, data analysis, and results were described in ANAMTHAWAT-JONSSON et al (1999). The AFLP profiles clearly show species differentiation since a number of bands are specific to only one of the two species (Fig.…”

Section: Icelandic Leymus Arenarius and Alaskan L Mollismentioning

confidence: 99%

Genetic and Genomic Relationships in Leymus Hochst.

Anamthawat‐Jónsson

2004

Hereditas

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Genetic and genomic relationships among three taxonomically related species of Leymus, northern European L. arenarius (octoploid, 2n = 56), northern American/Pacific L. mollis (tetraploid, 2n = 28) and central Eurasian L. racemosus (tetraploid, 2n = 28), were examined using molecular and cytogenetic methods. The amplified fragment length polymorphism (AFLP) analysis clearly differentiated Icelandic populations of L. arenarius from Alaskan populations of L. mollis. The former group is more genetically homogeneous than the latter. Leymus arenarius in Iceland has a common gene pool and a relatively recent origin. The Alaskan L. mollis, on the other hand, is probably a glacial survival that has accumulated high level of genetic variation and has differentiated into subspecies. Analysis of the 18S-26S ribosomal genes, by restriction fragment length polymorphism (RFLP) and fluorescence in situ hybridization (FISH), revealed a very close relationship between the octoploid northern European L. arenarius and the tetraploid Eurasian L. racemosus, such that the former could have originated from the latter, probably via interspecific hybridization. Leymus-specific DNA sequences were isolated and used for analyzing genetic relatedness among five Leymus species and four Psathyrostachys species. The RFLP analysis of retrotransposon sequence pLm44 and ribosomal clone pTa71 clearly revealed a close relationship between these two genera, i.e. higher variation was found within genera than between them. The results support the previous notion that Leymus is autopolyploid having all genomes being designated Ns as in Psathyrostachys, but a major taxonomic revision of this group would require analysis of more species.

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Molecular variation in Leymus species and populations

Cited by 15 publications

References 16 publications

Identifying the genome of wood barley Hordelymus europaeus (Poaceae: Triticeae)

Identifying the genome of wood barley Hordelymus europaeus (Poaceae: Triticeae)

Variations in abundance of 2 repetitive sequences in Leymus and Psathyrostachys species

Genetic and Genomic Relationships in Leymus Hochst.

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