Molecular variability among<i>Exserohilum turcicum</i>isolates using RAPD (random amplified polymorphic DNA)

Abadi, Rachel; Perl‐Treves, Rafael; Levy, Y.

doi:10.1080/07060669609500651

Cited by 9 publications

(7 citation statements)

References 12 publications

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“…The indexes derived from 13 race groups varied from 20.31 to 82.81% (Table 5). These results revealed that great genetic differentiation generated between different race groups, which agreed with the conclusions of previous studies [8] . In order to elucidate the phylogenetic relationships between race groups, a phylogenetic dendrogram was generated by the UPGMA method [17] based on the average similarity coefficient between race groups using the SAHN program of the NTSYS-pc package [18] .…”

Section: Variation Between Racessupporting

confidence: 82%

“…The use of RAPD (random amplification of polymorphic DNA) markers was widely used in taxonomy and genetic variance of microbial populations and was ever applied to study the genetic polymorphism of S. turcica in Africa and Isalel [8] , in tropical and temperate [9] and in Europe [10] . The studies enhanced the ability to make inferences about phylogeny and provided vital information about possible genetic mechanisms of S. turcica.…”

Section: Setosphaeria Turcica Leonard and Suggs Anamorphmentioning

confidence: 99%

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Geographic Distribution and Genetic Analysis of Physiological Racesof Setosphaeria turcica in Northern China

Dong¹,

Fan²,

Xiumei³

et al. 2008

American J. of Agricultural and Biological Sciences

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Five hundreds and forty-six isolates of Setosphaeria turcica, the causal agent of Northern Corn Leaf Blight, were collected in 61 corn-growing locations throughout six provinces of Northern China during [2000][2001][2002], to determine their pathogenicity on two sets of host differentials: OH43/Huangzao4, OH43Ht1Huangzao4Ht1, OH43Ht2/Huangzao4Ht2, OH43Ht3/Huangzao4Ht3 and OH43HtN/Huangzao4HtN. The isolates were grouped into 13 different physiological races (0, 1, 12, 3, 13, 23, N, 1N, 2N, 12N, 3N, 23N and 123N) based on their infection types on the host differentials. Incidence analyses indicated that races 0 and race 1 were still dominant in Northern China and took 40.66% and 18.32% of total isolates tested respectively, while other races sparsely occurred from 1.28-6.59%. The emergence of race 123N that was toxic to corn lines woth all four existing major resistant genes implies the possibility of present hybrids to loss their resistance in some regions of China. Further analysis of race distribution in Northern China demonstrated that the occurrence and composition of physiological races of S. turcica varied among provinces. Genetic analysis of within and between pathogenic race groups by random amplification polymorphic DNA (RAPD) markers revealed high genetic diversity in S. turcica population. Relatively high genetic similarity (70.46-95.12%) was identified within race groups and the results suggested that race groups were genetically similar within one geographic locations, but genetic migration could possibly happen between some locations which might lead to relative high genetic diversity within one geographic location. The similarity indexes derived from 13 race groups varied from 20.31 to 82.81% indicating great genetic variation between race groups. The UPGMA dendrograms generated by NTSys software grouped the 13 races into two not very robust but relatively distinct clusters: cluster I (0, 1, N, 12, 1N, 2N and 12N) and cluster II (3, 13, 23, 3N, 23N and 123N) with 63.7 and 72.3% P value respectively. The cluster analyses suggested that the pathogen might have a great genetic change while mutating to be virulent Ht3 resistant gene, but the details about the mechanisms were remain unaware.

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Section: Variation Between Racessupporting

confidence: 82%

Section: Setosphaeria Turcica Leonard and Suggs Anamorphmentioning

confidence: 99%

Geographic Distribution and Genetic Analysis of Physiological Racesof Setosphaeria turcica in Northern China

Dong¹,

Fan²,

Xiumei³

et al. 2008

American J. of Agricultural and Biological Sciences

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“…In sorghum, three RAPD markers that are tightly linked to a locus for resistance to NCLB have been discovered [ 10 ]. Little is known about the variations in isolates of E. turcicum at the molecular level [ 11 ]. However, according to Mathur [ 12 ], there are no such reports on the existence of various E. turcicum races in sorghum.…”

Section: Introductionmentioning

confidence: 99%

Molecular and Morphological Characterization of Exserohilum turcicum (Passerini) Leonard and Suggs Causing Northern Corn Leaf Blight of Maize in Bihar

et al. 2022

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Maize is considered the third most important cereal crop in Asia after rice and wheat. Many diseases affect this crop due to the cultivation of various hybrids. This research aimed to characterize the causative agent of northern corn leaf blight disease in Bihar, India, caused by Exserohilum turcicum (Passerini) Leonard and Suggs. Leaf samples were collected from infected fields in five maize growing districts of Bihar in 2020–2022. A total of 45 fungal isolates from 135 samples were examined for cultural, morphological, and molecular characteristics and were identified as E. turcicum. The isolates were grouped into four groups based on colony color, i.e., olivaceous brown, blackish brown, whitish black, and grayish, and into two groups based on regular and irregular margins. The conidial shapes were observed to be elongated and spindle-shaped with protruding hilum, with conidial septa ranging from 2–12. Similarly, conidial length varied from 52.94 μm to 144.12 μm. β-tubulin gene sequences analysis made it possible to verify the identities of fungal strains and the phylogenetic relationships of all isolates, which were clustered in the same clade. The β-tubulin gene sequences of all the isolates showed a high level of similarity (100%) with reference isolates from GenBank accession numbers KU670342.1, KU670344.1, KU670343.1, KU670341.1, and KU670340.1. The findings of this study will serve as a baseline for future studies and will help to minimize yield losses.

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“…The classification of the causal agents of "Helminthosporium" diseases is based on morphological and, to a lesser degree, on biochemical and physiological characteristics (Matsumura et al, 1988). Some authors have used molecular markers to study the relationship among these species (Bakonyi et al, 1995;Abadi et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Increasingly, molecular biology techniques have been used to explore genetic variability in fungi (Caligiorne et al, 1999a). However, there are still few published studies on the genetic relationships among species that cause "Helminthosporium" diseases (Bakonyi et al, 1995;Abadi et al, 1996). The RAPD technique (Welsh & McClelland, 1990;Williams et al, 1990) involve the use of arbitrary primers that anneal to genomic DNA at low temperature conditions.…”

Section: Introductionmentioning

confidence: 99%

Genetic variation among pathogens causing "Helminthosporium" diseases of rice, maize and wheat

et al. 2002

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Twenty isolates of four fungal species, agents of "Helminthosporium" diseases in cereals, were collected from different regions: nine Bipolarisoryzae isolated from rice (Oryza sativa), seven B.sorokiniana from wheat (Triticum aestivum), two B. maydis, and two Exserohilumturcicum from maize (Zea mays). The strains were compared by PCR-RFLP and RAPD analysis. Size polymorphism among the isolates in the ITS region comprising the 5.8 S rDNA indicated genetic differences among the isolates, while a UPGMA phenogram constructed after the digestion of this region with restriction enzymes showed inter- and intra-specific polymorphism. The RAPD profiles indicated an expressive level of polymorphism among different species, compared with a low level of polymorphism among isolates of the same species. A UPGMA phenogram grouped the isolates according to the species and their host plant. RAPD profiles did not reveal polymorphism that directly correlated climatic factors with geographic source of the isolates of B. sorokiniana, and B. oryzae. Teleomorphic species revealed high similarity with their correspondent anamorphs.

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Molecular variability amongExserohilum turcicumisolates using RAPD (random amplified polymorphic DNA)

Cited by 9 publications

References 12 publications

Geographic Distribution and Genetic Analysis of Physiological Racesof Setosphaeria turcica in Northern China

Geographic Distribution and Genetic Analysis of Physiological Racesof Setosphaeria turcica in Northern China

Molecular and Morphological Characterization of Exserohilum turcicum (Passerini) Leonard and Suggs Causing Northern Corn Leaf Blight of Maize in Bihar

Genetic variation among pathogens causing "Helminthosporium" diseases of rice, maize and wheat

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