Molecular typing of Mycobacterium tuberculosis circulated in Moscow, Russian Federation

Abstract

The present study investigates epidemiological diversity and multidrug resistance spreading among Mycobacterium tuberculosis strains circulating in Moscow, Russian Federation. Among 115 M. tuberculosis strains selected randomly from the sputum of epidemiologically unrelated tuberculosis (TB) patients, multidrug-resistant (MDR) strains predominated. Mutations in the RRDR of the rpoB gene were detected in 64 (83.1%) of 77 rifampicin (RIF)-resistant strains. The Ser531→Leu substitution was prevalent among them (76.5%). Aberrations in the Ser315 codon of katG and/or in the inhA promoter region were found in 79 (84.0%) of 94 isoniazid (INH)-resistant strains. Strains belonging to the Beijing family prevailed. Seventy-one different patterns were identified using the 24-VNTR loci typing scheme. Three main 24-loci VNTR clusters included 34 strains which belonged to the Beijing family. The spoligotyping and 24-loci VNTR typing combination demonstrated maximal discriminatory power. Among the Beijing strains, the MDR phenotype was revealed more frequently than among the others. High genetic heterogeneity of the studied population was shown by the assessment of VNTR loci variability in the analyzed group and in the strains from other parts of Russia. Comparison of the 24-VNTR locus typing and spoligotyping data with revealed resistance-associated mutation allows us to make a suggestion that the active transmission of MDR strains and the independent appearance of drug resistance during chemotherapy occurred in the studied population simultaneously.

Mycobacterium tuberculosis Population in Northwestern Russia: An Update from Russian-EU/Latvian Border Region

“…Prison-based studies in Kyrgyzstan [21] and Georgia [20] found very high rate of Beijing/M2 suggesting a particular capacity of this Beijing subtype to spread in a prison ( = overcrowding) environment. On the other hand, these findings were not replicated in the Russian study in the Moscow setting [24]. Thus the relation of the M2 or M11 MIRU-types to the increased transmissibility remains uncertain.…”

Genotypic characteristics of Mycobacterium tuberculosis circulating in Xinjiang, China

“…The proportion of Beijing/W lineage strain infections has been reported to be 91.82% in Beijing [20], with similar predominance in provinces that neighbor Xinjiang [21,22] (69.34% in Sichuan, 96.3% in Tibet, 87.58% in Gansu, and 93.26% in Inner Mongolia). However, the Beijing/W lineage is less prevalent in countries that neighbor Xinjiang (6.09% in Russia [23], 50% in Mongolia [4], and 6% in Pakistan [24]). In the present study, we found that Beijing/W lineage strains accounted for 57.48% (219/381) of isolates from the cases in Xinjiang, which is lower than that of neighboring provinces, but higher than that of neighboring countries.…”

Tuberculose Multirresistente por Estirpes da Família Beijing, em Doentes de Lisboa, Portugal: Estudo Preliminar

“…10,11,15,23 Entretanto, na região de Lisboa desconhece-se o que possa ter acontecido com estas estirpes, nomeadamente, nos primeiros anos da epidemia de TB MR, em que predominavam os co-infectados por VIH, os UDI, a TB MR secundária à má adesão ao tratamento e a família Lisboa. 36,38 Nos cinco doentes do estudo não identificámos quaisquer manifestações clínicas ou sinais radiológicos, que fossem atributos da TB causada por esta família Beijing quer nos doentes imunocompetentes, quer no co-infectado por VIH, todos eles com TB pulmonar, contudo é de salientar a frequência das cavitações.…”

“…[4][5][6][7][8] Num estudo efectuado numa província chinesa, 90% das estirpes eram Beijing e, destas, 27% eram multirresistentes (MR) e no Japão e em Taiwan representaram, respectivamente, 70% das estirpes extensivamente resistentes (ER) e 48% das estirpes MR. [9][10][11] A família Beijing tem, também, grande prevalência na Rússia onde se documentou que era responsável por 48% dos casos de TB MR e por transmissão activa na comunidade. [12][13][14][15] O seu aparecimento nos Estados Unidos da América (EUA) foi, de igual modo, bem demonstrado. 16,17 Em Nova Iorque, a estirpe W da família Beijing emergiu no início dos anos 90 e foi responsável por grandes epidemias em hospitais e prisões, associadas à infecção por vírus da imunodeficiência humana (VIH), à multirresistência, a grande rapidez de transmissão e a tratamento complicado.…”