Genotypic characteristics of<i>Mycobacterium tuberculosis</i>circulating in Xinjiang, China

Li, Yuan; Mi, Ligu; Li, Yongxiang; Zhang, Hui; Zheng, Fang; Li, Zhuoya

doi:10.3109/23744235.2015.1087649

Cited by 10 publications

(9 citation statements)

References 25 publications

(24 reference statements)

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“…The Beijing genotype family is the dominant lineage in southern Xinjiang, accounting for 58.4% of strains in the present study, which is in line with Yuan et al’s report from Xinjiang, China 31 and the data from the south of China (53.2%), 32 but lower than that from the north of China (76.5%). 32 According to subtype analysis with SITs, the most predominant clusters were SIT 1 (Beijing), SIT 127 (Ural-2), SIT 25 (CAS1-Delhi), SIT 26 (CAS1-Delhi), SIT 357 (CAS1-Delhi) and SIT 27 (EAI).…”

Section: Discussionsupporting

confidence: 93%

Prevalence and Molecular Characteristics Based on Whole Genome Sequencing of Mycobacterium tuberculosis Resistant to Four Anti-Tuberculosis Drugs from Southern Xinjiang, China

Anwaierjiang¹,

Wang²,

Liu³

et al. 2021

IDR

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Drug-resistant tuberculosis is a major public health problem, especially in the southern region of Xinjiang, China; however, there is little information regarding drug resistance profiles and mechanism of Mycobacterium tuberculosis in this area. The aim of this study was to determine the prevalence and molecular characteristics of M. tuberculosis resistant to four anti-tuberculosis drugs from this area. Methods: Three hundred and forty-six isolates from the southern region of Xinjiang, China were included and used to perform phenotypic drug susceptibility testing and whole genome sequencing (WGS). Mutations in seven loci associated with drug resistance, including rpoB for rifampicin (RMP), katG, inhA promoter and oxyR-ahpC for isoniazid (INH), rrs 530 and 912 loops and rpsL for streptomycin (STR), and embB for ethambutol (EMB), were characterized. Results: Among 346 isolates, 106, 60, 70 and 29 were resistant to INH, RMP, STR and EMB, respectively; 132 were resistant to at least one of the four anti-tuberculosis drugs and 51 were multi-drug resistant (MDR). Beijing genotype and retreated patients showed a significantly increased risk for developing MDR tuberculosis. Compared with the phenotypic data, the sensitivity and specificity for WGS to predict resistance were 96.7% and 98.6% for RMP, 75.5% and 97.1% for INH, 68.6% and 99.6% for STR, 93.1% and 93.7% for EMB, respectively. The most common mutations conferring RMP, INH, STR and EMB resistance were Ser450Leu (51.7%) in rpoB, Ser315Thr (44.3%) in katG, Lys43Arg (35.7%) in rpsL and Met306Val (24.1%) in embB. Conclusion:This study provides the first information on the prevalence and molecular characters of drug resistant M. tuberculosis in the southern region of Xinjiang, China, which will be helpful for choosing early detection methods for drug resistance (ig, molecular methods) and subsequently initiation of proper therapy of tuberculosis in this area.

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Section: Discussionsupporting

confidence: 93%

Prevalence and Molecular Characteristics Based on Whole Genome Sequencing of Mycobacterium tuberculosis Resistant to Four Anti-Tuberculosis Drugs from Southern Xinjiang, China

Anwaierjiang¹,

Wang²,

Liu³

et al. 2021

IDR

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“…The Beijing family strain was also found to be the most common genotype in Shanxi (81.5%) and Yunnan (70.5%), which was related to an increased risk of MDR-TB ( 28 , 29 ). In Xinjiang, the Beijing lineage strains accounted for 57.48% of total M.tb isolates ( 30 ).…”

Section: Discussionmentioning

confidence: 99%

A Prospective Cohort Study on the Prevalent and Recurrent Tuberculosis Isolates Using the MIRU-VNTR Typing

Qiu

Tao

et al. 2021

Front. Med.

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The study aims to describe the clustering characteristics of Mycobacterium tuberculosis (M.tb) strains circulating in eastern China and determine the ratio of relapse and reinfection in recurrent patients. We recruited sputum smear-positive pulmonary tuberculosis cases from five cities of Jiangsu Province, China, during August 2013 and December 2015. Patients were followed for the treatment outcomes and recurrence based on a cohort design. M.tb strains were isolated and genotyped using the 12-locus MIRU-VNTR. The Beijing family was identified by the extended Region of Difference (RD) analysis. The Hunter-Gaston Discriminatory Index (HGDI) was used to judge the resolution ability of MIRU-VNTR. The odds ratio (OR) together with 95% confidence interval (CI) were used to estimate the strength of association. We performed a cluster analysis on 2098 M.tb isolates and classified them into 545 genotypes and five categories (I, 0.19%; II, 0.43%; III, 3.34%; IV, 77.46%; V, 18.59%). After adjusting for potential confounders, the Beijing family genotype (OR = 118.63, 95% CI: 79.61–176.79, P = 0.001) was significantly related to the dominant strain infections. Patients infected with non-dominant strains had a higher risk of the pulmonary cavity (OR = 1.39, 95% CI: 1.01–1.91, P = 0.046). Among 37 paired recurrent cases, 22 (59.46%) were determined as endogenous reactivation, and 15 (40.54%) were exogenous reinfection. The type of M.tb strains prevalent in Jiangsu Province is relatively single. Beijing family strains infection is dominant in local tuberculosis cases. Endogenous reactivation appears to be a major cause of recurrent tuberculosis in Eastern China. This finding emphasizes the importance of case follow-up and monitoring after the completion of antituberculosis treatment.

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“…MIRU provides sufficient detail to discriminate isolates through 24 fast-evolving markers of size variation [ 12 , 13 ], whilst also providing an approximate strain classification in the global M. tuberculosis tree [ 9 , 10 ]. 24-loci MIRU-VNTR also has the advantages of genotyping simplicity and low cost which renders it the current gold standard for large population-based TB transmission studies and molecular epidemiological studies worldwide [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

A complex scenario of tuberculosis transmission is revealed through genetic and epidemiological surveys in Porto

Rito

Matos²,

Carvalho

et al. 2018

BMC Infect Dis

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BackgroundTuberculosis (TB) incidence is decreasing worldwide and eradication is becoming plausible. In low-incidence countries, intervention on migrant populations is considered one of the most important strategies for elimination. However, such measures are inappropriate in European areas where TB is largely endemic, such as Porto in Portugal. We aim to understand transmission chains in Porto through a genetic characterization of Mycobacterium tuberculosis strains and through a detailed epidemiological evaluation of cases.MethodsWe genotyped the M. tuberculosis strains using the MIRU-VNTR system. We performed an evolutionary reconstruction of the genotypes with median networks, used in this context for the first time. TB cases from a period of two years were evaluated combining genetic, epidemiological and georeferencing information.ResultsThe data reveal a unique complex scenario in Porto where the autochthonous population acts as a genetic reservoir of M. tuberculosis diversity with discreet episodes of transmission, mostly undetected using classical epidemiology alone.ConclusionsAlthough control policies have been successful in decreasing incidence in Porto, the discerned complexity suggests that, for elimination to be a realistic goal, strategies need to be adjusted and coupled with a continuous genetic characterization of strains and detailed epidemiological evaluation, in order to successfully identify and interrupt transmission chains.

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Genotypic characteristics ofMycobacterium tuberculosiscirculating in Xinjiang, China

Cited by 10 publications

References 25 publications

Prevalence and Molecular Characteristics Based on Whole Genome Sequencing of Mycobacterium tuberculosis Resistant to Four Anti-Tuberculosis Drugs from Southern Xinjiang, China

Prevalence and Molecular Characteristics Based on Whole Genome Sequencing of Mycobacterium tuberculosis Resistant to Four Anti-Tuberculosis Drugs from Southern Xinjiang, China

A Prospective Cohort Study on the Prevalent and Recurrent Tuberculosis Isolates Using the MIRU-VNTR Typing

A complex scenario of tuberculosis transmission is revealed through genetic and epidemiological surveys in Porto

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