Molecular typing of MRSA isolates by spa and PFGE

Alkharsah, Khaled R.; Rehman, Suriya; Alnimr, Amani; Diab, Asim; Hawwari, Abbas; Tokajian, Sima

doi:10.1016/j.jksus.2018.07.018

Cited by 25 publications

(15 citation statements)

References 34 publications

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“…The use of these typing tools contributes to understanding clonal diversity and transmission of MRSA in the hospital and community settings [124]. Infrequently, when misclassification or non-typeability is a concern, sophisticated typing tools, such as PFGE and MLST, are informative supplementary additions [125,126].…”

Section: Mrsa Typing Methods and Techniquesmentioning

confidence: 99%

Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review

Mairi

Touati

Lavigne

2020

Toxins

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This review assessed the molecular characterization of the methicillin-resistant Staphylococcus aureus (MRSA)-ST80 clone with an emphasis on its proportion of total MRSA strains isolated, PVL production, spa-typing, antibiotic resistance, and virulence. A systematic review of the literature was conducted on MRSA-ST80 clone published between 1 January 2000 and 31 August 2019. Citations were chosen for a review of the full text if we found evidence that MRSA-ST80 clone was reported in the study. For each isolate, the country of isolation, the sampling period, the source of isolation (the type of infection, nasal swabs, or extra-human), the total number of MRSA strains isolated, number of MRSA-ST80 strains, antibiotic resistance patterns, PVL production, virulence genes, and spa type were recorded. The data from 103 articles were abstracted into an Excel database. Analysis of the data showed that the overall proportion of MRSA-ST80 has been decreasing in many countries in recent years. The majority of MRSA-ST80 were PVL positive with spa-type t044. Only six reports of MRSA-ST80 in extra-human niches were found. This review summarizes the rise of MRSA-ST80 and the evidence that suggests that it could be in decline in many countries.

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Section: Mrsa Typing Methods and Techniquesmentioning

confidence: 99%

Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review

Mairi

Touati

Lavigne

2020

Toxins

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“…Identification by polymerase chain reaction (PCR) [41,59,[62][63][64][65][66][67][68] is also widely used. Specie confirmation can be performed using nuc gene; mecA gene is used to identify the methicillin-resistant strain [59,62,63,65,67,68].…”

Section: Methods Used For Identification and Characterization Of Mrsamentioning

confidence: 99%

“…After confirmation of the mecA gene, it is important to submit the strains to additional molecular characterization. The techniques that are generally used are as follows: staphylococcal protein A (spa) typing [59,61,[66][67][68], multilocus sequence typing (MLST) [59,61,64,66,67,70], pulsed field gel electrophoresis (PFGE) [64][65][66], Staphylococcal cassette chromosome mec (SCCmec) typing [59,66].…”

Section: Methods Used For Identification and Characterization Of Mrsamentioning

confidence: 99%

Methicillin-resistant Staphylococcus aureus in food and the prevalence in Brazil: a review

2019

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Foodborne diseases (FBD) occur worldwide and affect a large part of the population, being a cause of international concern among health authorities. Staphylococcus aureus can be transmitted by contaminated food, and it is one of the pathogens that most cause foodborne outbreaks in Brazil. Currently, this organism's ability in developing resistance to antibiotics is notorious; methicillin-resistant Staphylococcus aureus-MRSA-is known for its resistance to methicillin, oxacillin, and others. MRSA is one of the leading causes of infections, becoming a major threat to human health worldwide due to the numerous toxins that can produce. At first, the transmission of MRSA occurred in clinical environments; but in recent decades, its presence has been reported in the community, outside the hospital environment, including food and food-producing animals around the world. In this review, information about MRSA was gathered to verify MRSA incidence in the world but especially in Brazil in food samples, food handlers, food-producing animals, and food processing environments. The studies show that MRSA is easily found and in certain cases with high frequency, thus representing a potential risk to public health.

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“…Collectively, this would all suggest an acquisition of a concurrent distribution of both community-and hospital-associated MRSA in nursing homes. Further studies using well-established methods such as pulse-field gel electrophoresis (PFGE) or staphylococcal protein A (spa) typing methods to genotype MRSA isolates would facilitate determining strain relatedness, evolutionary patterns, and source of transmission more accurately [27]. e limitations of our study were mainly driven by including a relative small number of subjects especially with respect to MRSA colonization.…”

Section: Canadian Journal Of Infectious Diseases and Medical Microbiomentioning

confidence: 99%

Prevalence and Molecular Genetics of Methicillin-Resistant Staphylococcus aureus Colonization in Nursing Homes in Saudi Arabia

Albarrag

Shami

Almutairi

et al. 2020

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Objective. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main causative agents of nosocomial infections that has posed a major threat to those with compromised immune systems such as nursing home residents. The aim of this study was to determine the rates of MRSA strains and the types of Staphylococcal Cassette Chromosome mec (SCCmec)in nursing homes in Saudi Arabia. Methods. A total of 188 nasal swabs were collected from the residents and nursing staff in two nursing homes in Riyadh, Saudi Arabia. All MRSA isolates were tested for antimicrobial susceptibility and analyzed for mecA and SCCmec typing by multiplex PCR assay. Detection of the Panton–Valentine leukocidin (PVL) gene was also tested in all positive MRSA isolates by multiplex PCR using specific primers. Results. Among the 188 collected nasal swabs (105 males and 83 females), MRSA colonization rate was 9.04% (11 (5.85%) females and 6 (5.71%) males). About 47% of MRSA were multidrug resistant (MDR) as acquired resistance to beta-lactam, macrolide, and aminoglycoside antibiotics. However, all the MRSA isolates showed susceptibility to vancomycin, tigecycline, and linezolid. All the MRSA isolates (n = 17) were mecA-positive with the SCCmec IVc (n = 7, 41.18%) as the most common SCCmec type followed by SCCmec V (n = 5, 29.41%) and SCCmec IVa (n = 2, 11.76%). The remaining isolates (n = 3) were nontypeable (17.65%). In addition, the PVL toxin gene was only detected in four of the male samples. Conclusion. MRSA nasal colonization is a common incident among nursing home residents. The prevalence of community-associated (CA) MRSA (SCCmec IV and V) was more common than hospital-associated (HA) MRSA in our study samples. It is crucial to investigate such rate of incidence, which is a key tool in preventive medicine and would aid in determining health policy and predict emergent outbreaks.

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Molecular typing of MRSA isolates by spa and PFGE

Cited by 25 publications

References 34 publications

Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review

Methicillin-Resistant Staphylococcus aureus ST80 Clone: A Systematic Review

Methicillin-resistant Staphylococcus aureus in food and the prevalence in Brazil: a review

Prevalence and Molecular Genetics of Methicillin-Resistant Staphylococcus aureus Colonization in Nursing Homes in Saudi Arabia

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