Molecular Typing of Methicillin-Resistant Staphylococcus Aureus Strains by PCR-RFLP of Spa Gene: A Reference Laboratory Perspective

Abstract

The study demonstrates the importance of spa genotyping in the discrimination of MRSA strains, which were otherwise indistinguishable by bacteriophage typing. spa genotyping allowed differentiation of strains within a particular phage type. Nucleotide sequencing of isolates of different PCR-RFLP patterns indicated a correlation between the RFLP patterns of a variable number of tandem repeats and the phage type. The study provides valuable information on the epidemiological characterization of MRSA strains.

New <i>Spa</i> Types among MRSA and MSSA Isolates in North of Iran

“…In another survey conducted in India by this method only 5 spa types were detected [14], but in this present study spa typing was carried out based on its Xr region sequencing and found about 50 spa types. This means PCR-RFLP spa gene had lower discriminative effect than Xr spa sequencing method, therefore Xr spa sequence typing preferred to PCR-RFLP method of spa gene.…”

Association of Virulence Factor (Panton–valentine Leukocidin) With Meca Gene in Staphylococcus Aureus Isolates in Tertiary Care Hospital

“…Molecular typing techniques have been used with increasing frequency in studies of the epidemiology of MRSA and also for rapid detection of MRSA clones and virulent genes also [10][11][12].…”

Evaluation of LAMP Assay Using Phenotypic Tests and Conventional PCR for Detection of nuc and mecA genes Among Clinical Isolates of Staphylococcus spp

“…Staphylococcus aureus (S.aureus) is one of the major pathogens causing community and health care associated infections [1,2]. The clinical manifestations of S.aureus ranges from local infections to severe life threatening systemic manifestations such as bacteremia, toxic shock syndrome [3].…”