Molecular Typing of
            <i>Campylobacter jejuni</i>
            Isolates Involved in a Neonatal Outbreak Indicates Nosocomial Transmission

Llovo, J.; Mateo, Estíbaliz; Muñoz, Ana; Urquijo, María; On, Stephen L. W.; Fernández‐Astorga, Aurora

doi:10.1128/jcm.41.8.3926-3928.2003

Cited by 15 publications

(4 citation statements)

References 14 publications

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“…Campylobacter spp. show a remarkable ability to resist a wide spectrum of antimicrobial agents, resulting in widespread nosocomial outbreaks throughout the world [7,8]. Moreover, the widespread and empirical use of broad-spectrum antibiotics including erythromycin, ciprofloxacin, kanamycin, nalidixic acid, and chloramphenicol in human, as well as in agricultural and aquacultural settings, has led to the emergence of multidrug-resistant (MDR) Campylobacter spp., which poses a major challenge to clinical therapy and contributes significantly to the increased morbidity and mortality rates [9,10].…”

Section: Introductionmentioning

confidence: 99%

Significant contribution of the CmeABC Efflux pump in high-level resistance to ciprofloxacin and tetracycline in Campylobacter jejuni and Campylobacter coli clinical isolates

Sharifi

Bakhshi

Peerayeh

2021

Ann Clin Microbiol Antimicrob

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Background Campylobacter resistance to antimicrobial agents is regarded as a major concern worldwide. The aim of this study was to investigate the expression of the CmeABC efflux pump and the RAPD-PCR pattern in drug-resistant Campylobacter isolates. Methods A total of 283 stool specimens were collected from children under the age of five with diarrhea. The minimum inhibitory concentration (MIC) of tetracycline and ciprofloxacin was determined by broth microdilution method and E-test, respectively. Detection of tetracycline and ciprofloxacin determinants was done by amplification of tetO gene and PCR-sequencing of the gyrA gene. The cmeABC transcriptional expression was analyzed by Real-time (RT)-PCR. Clonal correlation of resistant strains was determined by RAPD-PCR genotyping. Results Out of 283 fecal samples, 20 (7.02%) samples were positive for Campylobacter spp. Analysis of duplex PCR assay of the cadF gene showed that 737 and 461 bp amplicons were corresponding to Campylobacter jejuni and Campylobacter coli, respectively. All of the 17 phenotypically tetracycline-resistant Campylobacter isolates harbored the tetO gene. Also, four phenotypically ciprofloxacin-resistant Campylobacter isolates had a point mutation at codon 257 of the gyrA gene (ACA to ATA; Thr > Ile). High-level expression of the cmeA gene was observed in ciprofloxacin-resistant and high-level tetracycline-resistant Campylobacter isolates, suggesting a positive correlation between the cmeA gene expression level and tetracycline resistance level. Moreover, a statistically significant difference was observed in the cmeA gene expression between ciprofloxacin-resistant and ciprofloxacin-susceptible strains, which signifies the crucial contribution of the efflux pump in conferring multiple drug resistance phenotype among Campylobacter spp. RAPD analysis of Campylobacter isolates exhibited 16 different patterns. Simpsone`s diversity index of RAPD-PCR was calculated as 0.85, showing a high level of homogeneity among the population; however, no clear correlation was detected among tetracycline and/or ciprofloxacin resistant isolates. Conclusion Significant contribution of the CmeABC efflux pump in conferring high-level resistance to tetracycline and ciprofloxacin was observed in C. jejuni and C. coli clinical isolates. The resistant phenotype is suggested to be mediated by CmeABC efflux pumps, the tetO gene, and point mutation of the gyrA gene. Genotyping revealed no clonal correlation among resistant strains, indicating distinct evolution of tetracycline and ciprofloxacin resistant genotypes among the isolates.

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Section: Introductionmentioning

confidence: 99%

Significant contribution of the CmeABC Efflux pump in high-level resistance to ciprofloxacin and tetracycline in Campylobacter jejuni and Campylobacter coli clinical isolates

Sharifi

Bakhshi

Peerayeh

2021

Ann Clin Microbiol Antimicrob

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“…Macrolides remain the drug of choice for the treatment of Campylobacter spp enteritis [1,6,11]. However, resistance rates (3-11%) have been reported worldwide [6].…”

Section: Discussionmentioning

confidence: 99%

Campylobacter jejuni meningitis in a neonate: A rare case report

Tsoni¹,

Papadopoulou²,

Michailidou³

et al. 2013

Journal of Neonatal-Perinatal Medicine

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“…Por ser um micro-organismo microaerófilo, o Campylobacter é considerado de difícil cultivo (PRESTON; PENNER, 1989), além da insuficiência de intervenções estratégicas para a redução da contaminação das carcaças de frango pelo agente (PATTISON, 2001). Por outro lado, os estudos de sua taxonomia, epidemiologia, métodos microbiológicos convencionais, biologia molecular e patogenia estão acelerados (LLOVO et al, 2003;SALEHA, 2002). A rastreabilidade da fonte de origem é importante no esclarecimento da contaminação dos lotes de frango de corte, na higiene alimentar e na prevenção de doenças veiculadas por alimentos (CARVALHO et al, 2001).…”

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Sistema Api Campy Para Caracterização De Amostras De Campylobacter Isoladas De Descarga Cecal, Fezes, Swabs Cloacais E Carcaças De Frangos De Corte

et al. 2009

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RESUMO O presente estudo verificou a aplicação do sistema API Campy para identificação de bactérias do gênero Campylobacter. Foram utilizadas 48 amostras, sendo 15 oriundas de descarga cecal, cinco de fezes, três de swabs cloacais e 21 de carcaças, isoladas de 22 lotes de frangos de corte com três semanas de idade. As amostras caracterizadas presuntivamente por microscopia em contraste de fase, coloração de Gram, catalase/oxidase e aglutinação em látex foram inoculadas no sistema API Campy, que consta de testes enzimáticos e convencionais em aerobiose e testes de assimilação ou inibição em microaerofilia, ambos incubados a 37º C por 24-48 horas, sendo a leitura realizada em sistema informatizado. Foi possível identificar 43 amostras (89,58%), enquanto cinco (10,41%) tiveram perfil inaceitável. Identificou-se as espécies C. jejuni subsp. jejuni (68,8%); C. coli (8,3%); C. jejuni subsp. doylei (6,3%); C. upsaliensis (4,2%) e C. fetus subsp. fetus (2,1%). A ocorrência de Campylobacter nos lotes de frango de corte estudados foi de 81,8% e as espécies identificadas principalmente como Campylobacter jejuni subesp. jejuni pelo sistema API Campy, apresentando um maior número de espécies, subespécies e biotipos deCampylobacter dentre as amostras isoladas de carcaças de frango do que dentre as amostras clínicas isoladas nas granjas.

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Molecular Typing of Campylobacter jejuni Isolates Involved in a Neonatal Outbreak Indicates Nosocomial Transmission

Cited by 15 publications

References 14 publications

Significant contribution of the CmeABC Efflux pump in high-level resistance to ciprofloxacin and tetracycline in Campylobacter jejuni and Campylobacter coli clinical isolates

Significant contribution of the CmeABC Efflux pump in high-level resistance to ciprofloxacin and tetracycline in Campylobacter jejuni and Campylobacter coli clinical isolates

Campylobacter jejuni meningitis in a neonate: A rare case report

Sistema Api Campy Para Caracterização De Amostras De Campylobacter Isoladas De Descarga Cecal, Fezes, Swabs Cloacais E Carcaças De Frangos De Corte

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