Molecular typing and antimicrobial resistance of Salmonella enterica subspecies enterica serovar Choleraesuis isolates from diseased pigs in Japan

Asai, Tetsuo; Namimatsu, Takanori; Osumi, Takayuki; Kojima, Akira; Harada, Kazuki; Aoki, Hiroshi; Sameshima, Toshiya; Takahashi, Toshio

doi:10.1016/j.cimid.2008.08.004

Cited by 30 publications

(31 citation statements)

References 22 publications

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“…One of the serovars commonly involved in foodborne diseases, S. Typhimurium from chicken, was resistant to eight antimicrobials, the greatest number of resistant phenotypes. Lower rates (62%-65%) were mentioned in a number of studies focused on these specific strains [24,25] while higher rates of 84%-97% were mentioned less frequently [26]. Other studies concluded that this resistance prevalence for tetracycline and streptomycin is due to their frequent administration in veterinary medicine [27].…”

Section: Discussionmentioning

confidence: 99%

First study of Salmonella in meat in Romania

Mihaiu

Lapusan

Tanasuica

et al. 2014

J Infect Dev Ctries

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Introduction: The increasing antimicrobial resistance of Salmonella isolates is of major public health concern, but information regarding these aspects is still lacking in Romania. This study focused on a detailed and accurate investigation concerning prevalence, serotypes, and antimicrobial resistance patterns of Salmonella strains, isolated from pork and chicken meat, collected from all regions of Romania in 2011. Methodology: The research was conducted on 650 samples of chicken and pork meat collected from production units and retail markets located in various regions of Romania. A total of 149 Salmonella isolates were recovered (22.92%), serotyped, confirmed by PCR, and further tested for antimicrobial susceptibility. Results: Thirteen Salmonella serovars were identified; predominant serovars included Infantis, Typhimurium, Derby and Colindale. Multiple resistance was found in 83.22% (n = 124) of the isolates. The isolates were frequently resistant to tetracycline (80.53%), streptomycin (81.21%), sulfamethoxazole (87.25%), nalidixic acid (65.10%), and ciprofloxacin (42.95%). Additionally, a markedly lower resistance rate was observed for ampicillin (20.81%), chloramphenicol (16.78%), and ceftazidime (11.41%). Among 137 resistant Salmonella isolates, 35 different resistance patterns were found. Conclusion: A high prevalence of Salmonella spp. and a relatively high resistance rate to multiple antimicrobials was found. This data indicates that chicken and pork meat could constitute a source of human exposure to multidrug-resistant Salmonella and therefore could be considered a potential vehicle of resistant Salmonella foodborne diseases. Further actions are needed to succesfully implement a national surveillance program for better monitoring of these resistant pathogens.

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Section: Discussionmentioning

confidence: 99%

First study of Salmonella in meat in Romania

Mihaiu

Lapusan

Tanasuica

et al. 2014

J Infect Dev Ctries

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“…Since then, reports on salmonellae resistance to one or more antibiotics have increased substantially and resistance has emerged even to newer more potent antimicrobial agents (Montville and Matthews, 2008;Piddock, 2002). In addition, multidrug resistance in Salmonella has become a public health concern (Crump and Mintz, 2010;Asai et al, 2010;Singh et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Hussein¹,

Mohy-Eldin²,

Elmadiena³

et al. 2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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“…For S. Choleraesuis, Martina et al (1996) suggested that a combination of plasmid analysis and PFGE proved to be suitable for subtyping of S. Choleraesuis. Using PFGE, molecular characteristics of S. Choleraesuis isolates from pigs in the United States, Japan, and Taiwan have been investigated (Weide-Botjes et al 1996;Chang et al 2005;Asai et al 2010). In these studies, PFGE was used as the typing tool, and, in general, XbaI alone or in combination with other restriction enzymes, such as BlnI and SpeI, has been used.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the fact that S. Choleraesuis may transmit among pigs as well as from pigs to humans, it is important to establish the genotypes for swine isolates of this serotype (Chang et al 2005;Asai et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Improvement of strain discrimination by combination of RAPD with PFGE for the analysis of the swine isolates of Salmonella enterica serovar Choleraesuis

Chiu

Pang

Chen

et al. 2010

World J Microbiol Biotechnol

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Salmonella enterica serovar Choleraesuis (S. Choleraesuis) can cause salmonellosis in pigs and humans. Currently, the most common method used for the subtyping of this Salmonella serovar is pulsed field gel electrophoresis (PFGE) using XbaI as a DNA digestion enzyme. In this study, we compared and combined PFGE with the randomly amplified polymorphic DNA method, for the typing of 95 S. Choloraesuis strains isolated from diseased pigs. Using PFGE with XbaI, with AvrII, and with SpeI digested DNA, 29, 74, and 40 patterns, respectively, were obtained. Also, 53, 15, and 35 strains, respectively, belong to the major patterns X1, A1, and S1. When these three digestion patterns were combined, 83 PFGE pattern combinations were obtained. On the other hand, using RAPD with selected primer alone generated 76 patterns, and 11 strains which fell within a single X1A1S1 PFGE combination pattern were discriminated into 10 patterns. Thus, for S. Choloraesuis, PFGE with AvrII allowed higher discrimination than PFGE with XbaI, and some of the PFGE groupings obtained by combining the XbaI, AvrII and SpeI digestion patterns were further subdivided by the RAPD method.

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Molecular typing and antimicrobial resistance of Salmonella enterica subspecies enterica serovar Choleraesuis isolates from diseased pigs in Japan

Cited by 30 publications

References 22 publications

First study of Salmonella in meat in Romania

First study of Salmonella in meat in Romania

Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Improvement of strain discrimination by combination of RAPD with PFGE for the analysis of the swine isolates of Salmonella enterica serovar Choleraesuis

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