Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Hussein, A. M.; Mohy-Eldin, Halima S.; Elmadiena, Mayha M. Nor; Siddig, Marmar A. El

doi:10.5772/29928

Cited by 5 publications

(5 citation statements)

References 108 publications

(85 reference statements)

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“…in wastewater collected from the University of Nigeria, Nsukka wastewater treatment plant (WWTP). This is in consonance with the work done by El Hussein et al (2012) in Khartoum State, Sudan, who reported an occurrence of 11.09% of Salmonella species in wastewater. Conversely, a report by Howard et al (2004) showed that municipal wastewater having undergone an activated sludge process continued to bear Salmonella at high concentration; the treated water yielded a most probable number (MPN) of 45/100 ml.…”

Section: Discussionsupporting

confidence: 78%

Serotyping and molecular typing of Salmonella species isolated from wastewater in Nsukka, Nigeria

Dickson¹,

Chibuogwu²,

Ezeonu³

2016

Afr. J. Microbiol. Res.

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The objective of this investigation was to isolate and identify Salmonella serovars present in wastewater from the University of Nigeria, Nsukka (UNN) wastewater treatment plant and to evaluate the sensitivity and precision of different microbial typing methods (conventional and molecular), in identifying and characterizing Salmonella species. A total of 100 suspected Salmonella colonies on selective media (Salmonella-Shigella agar and MacConkey Agar) were subjected to biochemical testing. A total of 12 biochemically typical Salmonella isolates were identified and further characterized. Serotyping analysis further identified 3 (25%) of the isolates as Salmonella enterica serovar Limete. Salmonella specific (16S) polymerase chain reaction (PCR) assay validated the result obtained by serotyping, although 2 of the isolates could not be serotyped and were identified as rough strains. PCR assay produced positive amplifications of 574 bp of the 16S rRNA gene specific for Salmonella, while non-Salmonella serovars were negative (100%). Random amplified polymorphic DNA (RAPD-PCR) analysis revealed the genetic relatedness of Salmonella serovars isolated from wastewater. Primers 787 and RAPD2 identified 4 RAPD-binding patterns, while primer 1254 did not give any discriminatory pattern. Molecular analyses (16S PCR and RAPD) showed discriminatory power, reproducibility, easy interpretation and performance. It is therefore a promising alternative method for typing Salmonella species.

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Section: Discussionsupporting

confidence: 78%

Serotyping and molecular typing of Salmonella species isolated from wastewater in Nsukka, Nigeria

Dickson¹,

Chibuogwu²,

Ezeonu³

2016

Afr. J. Microbiol. Res.

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“…Our research shows that our LAB-CFCS are promising strategies for Salmonella biofilm inhibition (95–96% for S. Typhi and 46–75% for S. Typhimurium as shown in Table 3). Salmonella can grow in a broad pH range of 4–9, with the optimum being 6.5–7.5 and do not survive in acidic environments (El Hussein et al, 2012). Our tested LAB-CFCS were very effective to inhibit biofilm formation of both Salmonella serovars.…”

Section: Discussionmentioning

confidence: 99%

Cell-free supernatants from cultures of lactic acid bacteria isolated from fermented grape as biocontrol againstSalmonellaTyphi andSalmonellaTyphimurium virulence via autoinducer-2 and biofilm interference

Pelyuntha

Chaiyasut

Kantachote

et al. 2019

PeerJ

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Background Salmonella Typhi and Salmonella Typhimurium are the causative pathogens of salmonellosis, and they are mostly found in animal source foods (ASF). The inappropriate use of antibiotics enhances the possibility for the emergence of antibiotic resistance in pathogens and antibiotic residue in ASF. One promising alternative to antibiotics in animal farming is the use of lactic acid bacteria (LAB). Methods The present study was carried out the cells and/or the cell-free culture supernatants (CFCS) from beneficial LAB against S. Typhi and S. Typhimurium. The antibacterial mechanisms of LAB-CFCS as biocontrol agents against both Salmonella serovars were investigated through the analysis of anti-salmonella growth activity, biofilm inhibition and quorum quenching activity. Results Among 146 LAB strains isolated from 110 fermented food samples, the 2 strong inhibitory effect strains (WM33 and WM36) from fermented grapes against both Salmonella serovars were selected. Out of the selected strains, WM36 was the most effective inhibitor, which indicated S. Typhi by showing 95.68% biofilm inhibition at 20% biofilm inhibition concentration (BIC) and reduced 99.84% of AI-2 signaling interference. The WM33 was the best to control S. Typhimurium by producing 66.46% biofilm inhibition at only 15% BIC and 99.99% AI-2 signaling a reduction. The 16S rDNA was amplified by a polymerase chain reaction (PCR). The selected isolates were identified as Weissella viridescens WM33 and Weissella confusa WM36 based on nucleotide homology and phylogenetic analysis. Conclusion The metabolic extracts from Weissella spp. inhibit Salmonella serovars with the potential to be used as biocontrol agents to improve microbiological safety in the production of ASF.

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“…The presence of S. Limete was reported in water samples from 3 different farms in Gran Canaria, Spain [23]. Also, the serovar has also been recovered from human faeces in Sudan [24]. This broad host spectrum is a strong indication that contamination of the wastewater may be of either human and/or animal origin.…”

Section: Discussionmentioning

confidence: 96%

Detection of <i>invA</i> Gene in <i>Salmonella</i> Limete Isolated from Wastewater Treatment Plant of University of Nigeria Nsukka, Nigeria

Ike¹

2018

IJMB

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Salmonellae are ubiquitous microorganisms that infect both humans and animals. Human infections usually occur through contaminated food or water and can result in one of two major diseases, namely gastroenteritis and enteric fever. Hence, Salmonella remains a major public health problem especially in developing countries where the level of hygiene is very low. The objective of this study was to evaluate the potential risk of Salmonella serovars isolated from the University of Nigeria Nsukka (UNN) wastewater treatment plants. Three Salmonella enterica subspecies enterica serovar Limete isolates from the UNN waste treatment plants were investigated for the presence of invasive A (invA) gene. Deoxyribonucleic acid (DNA) was extracted from the isolates by boiling method. Extracted bacteria DNA was amplified by polymerase chain reaction (PCR) using invA specific primers. PCR products were resolved on 1.5% agarose gel stained with 0.5 µg/ml of ethidium bromide. Results showed the presence of a band size of 244 base pair of Salmonella invA gene in 2 of the isolates. This is an indication that the isolates may have a human or animal origin and are potentially pathogenic. Therefore, the treatment of water in the wastewater plant is insufficient and water from the plant should not be employed for human use or used with caution.

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Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Cited by 5 publications

References 108 publications

Serotyping and molecular typing of Salmonella species isolated from wastewater in Nsukka, Nigeria

Serotyping and molecular typing of Salmonella species isolated from wastewater in Nsukka, Nigeria

Cell-free supernatants from cultures of lactic acid bacteria isolated from fermented grape as biocontrol againstSalmonellaTyphi andSalmonellaTyphimurium virulence via autoinducer-2 and biofilm interference

Detection of <i>invA</i> Gene in <i>Salmonella</i> Limete Isolated from Wastewater Treatment Plant of University of Nigeria Nsukka, Nigeria

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Prevalence, Detection and Antimicrobial Resistance Pattern of Salmonella in Sudan

Cited by 5 publications

References 108 publications

Serotyping and molecular typing of Salmonella species isolated from wastewater in Nsukka, Nigeria

Serotyping and molecular typing of Salmonella species isolated from wastewater in Nsukka, Nigeria

Cell-free supernatants from cultures of lactic acid bacteria isolated from fermented grape as biocontrol againstSalmonellaTyphi andSalmonellaTyphimurium virulence via autoinducer-2 and biofilm interference

Detection of &lt;i&gt;invA&lt;/i&gt; Gene in &lt;i&gt;Salmonella&lt;/i&gt; Limete Isolated from Wastewater Treatment Plant of University of Nigeria Nsukka, Nigeria

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Detection of <i>invA</i> Gene in <i>Salmonella</i> Limete Isolated from Wastewater Treatment Plant of University of Nigeria Nsukka, Nigeria