Molecular tracing of the emergence, diversification, and transmission of
            <i>S. aureus</i>
            sequence type 8 in a New York community

Uhlemann, Anne‐Catrin; Dordel, Janina; Knox, Justin; Raven, Kathy E.; Parkhill, Julian; Holden, Matthew T. G.; Peacock, Sharon J.; Lowy, Franklin D.

doi:10.1073/pnas.1401006111

Cited by 167 publications

(232 citation statements)

References 49 publications

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“…It is unclear if any of the listed mutations are important for colonization of the nares or infection of the wound. Curiously, one of the mutations in the (30). The tree in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Most of the infections were due to a single MRSA clone, USA300, which is currently the most prevalent clone of CA-MRSA in the United States and specifically in New York City (30). ACME was restricted to strains belonging to the USA300 CA-MRSA clone or clones closely related to it.…”

Section: Discussionmentioning

confidence: 99%

“…The more complex tree was constructed with the software REALPHY using its default settings (http://realphy.unibas.ch/fcgi /realphy) (29). Using the vernacular in the documentation for REALPHY, the reference genome was USA300-FPR3757, the query genomes were the reads for the CAMP isolates (this study) and the northern Manhattan isolates (30), the two reference genome alignments were merged in order to generate a single tree, and the tree was inferred by PhyML. Accession numbers.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Types of Methicillin-Resistant Staphylococcus aureus and Methicillin-Sensitive S. aureus Strains Causing Skin and Soft Tissue Infections and Nasal Colonization, Identified in Community Health Centers in New York City

et al. 2015

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Network (CDN) launched a research and learning collaborative project with six community health centers in the New York City metropolitan area to determine the nature (clonal type) of community-acquired Staphylococcus aureus strains causing skin and soft tissue infections (SSTIs). Between November 2011 and March 2013, wound and nasal samples from 129 patients with active SSTIs suspicious for S. aureus were collected and characterized by molecular typing techniques. In 63 of 129 patients, the skin wounds were infected by S. aureus: methicillinresistant S. aureus (MRSA) was recovered from 39 wounds and methicillin-sensitive S. aureus (MSSA) was recovered from 24. Most-46 of the 63-wound isolates belonged to the CC8/Panton-Valentine leukocidin-positive (PVL ؉ ) group of S. aureus clone USA300: 34 of these strains were MRSA and 12 were MSSA. Of the 63 patients with S. aureus infections, 30 were also colonized by S. aureus in the nares: 16 of the colonizing isolates were MRSA, and 14 were MSSA, and the majority of the colonizing isolates belonged to the USA300 clonal group. In most cases (70%), the colonizing isolate belonged to the same clonal type as the strain involved with the infection. In three of the patients, the identity of invasive and colonizing MRSA isolates was further documented by whole-genome sequencing. Staphylococcus aureus is the most common cause of bacterial infections in humans worldwide (1), and methicillin-resistant Staphylococcus aureus (MRSA) is the main cause of skin and soft tissue infections (SSTIs) in North America, with a single clone, USA300, accounting for 98% of these infections (2, 3).The first human case of MRSA infection in the United States was reported in Boston, MA, in 1968 (4). MRSA was first detected in hospitals, and over the following decades, it became the main nosocomial pathogen around the world (5). In 1998, the prevalence of MRSA in 12 hospitals throughout the city of New York was assessed (6), and a single MRSA clone was found to be responsible for an overwhelming majority of MRSA infections. The same MRSA clone was subsequently identified as dominant in MRSA infections in 29 hospitals in the tristate area (7), and it was also identified in MRSA infections in Japan (8). This MRSA clone (multilocus sequence typing [MLST] clonal complex CC5, sequence type ST5, SCCmecII, and unique pulsed-field gel electrophoresis [PFGE] profile)-also known as the "New York/Japan clone" or "MRSA clone USA100"-became the most prevalent MRSA clone involved in MRSA infections in hospitals in the United States in the 1990s (9).In 1993, a new MRSA clone emerged in Kimberley, Western Australia (10), in a community of patients without previous health care contact (community-acquired MRSA [CA-MRSA]). In the late 1990s, CA-MRSA also appeared in the United States and was responsible for the death of four otherwise healthy pediatric patients in Minnesota and North Dakota (11). These new CA-MRSA strains belonged to a clone (USA400/CC1/SCCmecIV)

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“…It is unclear if any of the listed mutations are important for colonization of the nares or infection of the wound. Curiously, one of the mutations in the (30). The tree in Fig.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Types of Methicillin-Resistant Staphylococcus aureus and Methicillin-Sensitive S. aureus Strains Causing Skin and Soft Tissue Infections and Nasal Colonization, Identified in Community Health Centers in New York City

et al. 2015

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“…Clade CC8-A also carries the PVL-associated genes and contains isolates that belong to the USA300 clone. In a study investigating the genomics of USA300, Uhlemann et al found evidence for at least five different acquisitions of the lukSF-carrying prophage into the ST8 population, but only a single event into USA300 clone [47]. From their phylogenomic analysis, the authors concluded that acquisition of the lukSF-carrying prophage coincided with the acquisition of ACME and occurred between 1970 and 1993, resulting in the emergence of USA300.…”

Section: Community-associated Meticillin-resistant Staphylococcus Aurmentioning

confidence: 99%

Genomic insights into the emergence and spread of international clones of healthcare-, community- and livestock-associated meticillin-resistant Staphylococcus aureus : Blurring of the traditional definitions

Bal

Coombs

Holden

et al. 2016

Journal of Global Antimicrobial Resistance

120

105

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Please cite this article as: Bal AM, Coombs GW, Holden MTG, Lindsay JA, Nimmo GR, Tattevin P, Skov RL, Genomic insights into the emergence and spread of international clones of healthcare-, community-and livestock-associated meticillinresistant Staphylococcus aureus: blurring of the traditional definitions, Journal of Global Antimicrobial Resistance (2010), http://dx.doi.org/10.1016/j.jgar. 2016.04.004 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.  Genomics-based diagnostic tools such as whole-genome sequencing are useful in providing rapid information in relation to epidemiology and outbreaks.

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“…5) (9,(211)(212)(213). Comprehensive reviews address the essentially clonal population structure of S. aureus (214)(215)(216) and other staphylococcal species (207) and also the impact of HGT in the evolutionary history of staphylococcal populations (9,(217)(218)(219), with emphasis on the description of the plasmids associated with AbR genetic elements (9,51,84,220) and their influence on the variability of lineages (217,219,(221)(222)(223)(224).…”

Section: Staphylococcimentioning

confidence: 99%

The Plasmidome of Firmicutes: Impact on the Emergence and the Spread of Resistance to Antimicrobials

et al. 2015

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The phylum Firmicutes is one of the most abundant groups of prokaryotes in the microbiota of humans and animals and includes genera of outstanding relevance in biomedicine, health care, and industry. Antimicrobial drug resistance is now considered a global health security challenge of the 21st century, and this heterogeneous group of microorganisms represents a significant part of this public health issue.

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Molecular tracing of the emergence, diversification, and transmission of S. aureus sequence type 8 in a New York community

Cited by 167 publications

References 49 publications

Molecular Types of Methicillin-Resistant Staphylococcus aureus and Methicillin-Sensitive S. aureus Strains Causing Skin and Soft Tissue Infections and Nasal Colonization, Identified in Community Health Centers in New York City

Molecular Types of Methicillin-Resistant Staphylococcus aureus and Methicillin-Sensitive S. aureus Strains Causing Skin and Soft Tissue Infections and Nasal Colonization, Identified in Community Health Centers in New York City

Genomic insights into the emergence and spread of international clones of healthcare-, community- and livestock-associated meticillin-resistant Staphylococcus aureus : Blurring of the traditional definitions

The Plasmidome of Firmicutes: Impact on the Emergence and the Spread of Resistance to Antimicrobials

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