Molecular systematics of the genus Pleurotus: analysis of restriction polymorphisms in ribosomal DNA

Béatrice, Lraçabal; Georgios, Zervakis

doi:10.1099/13500872-141-6-1479

Cited by 30 publications

(4 citation statements)

References 41 publications

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“…Previous phylogenetic studies have given rise to various ambiguities in the genus Pleurotus . P. ostreatus , P. columbinus , and P. cornucopiae have been in turn associated in the same clade [ 36 ] or separated [ 34 ]. In our analysis, P. cornucopiae on clade III and P. columbinus and P. ostreatus on the clade V were included in two distinct clades.…”

Section: Resultsmentioning

confidence: 99%

Genetic and Chemical Diversity of Edible Mushroom Pleurotus Species

Lin

Yan

Kook

et al. 2022

BioMed Research International

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The genus Pleurotus is one of the most widely cultivated and edible mushrooms with various cultivators. Three molecular characteristics were used to evaluate the genetic diversity of 132 tested samples. Phylogenetic analysis showed five clades for tested samples of the genus Pleurotus by the combined ITS and LSU sequences with strong bootstraps and Bayesian posterior probability supports. A total of 94 polymorphic fragments ranging from 10 to 100 bp were observed by using an intersimple sequence repeat (ISSR) marker. The DNA fragment pattern showed that P. ostreatus cultivator (strain P9) was clearly distinguished from wild strain based on their clear banding profiles produced. DNA GC content of the genus Pleurotus varied from 55.6 mol% to 43.3 mol%. Their chemical composition was also determined, including sugar, amino acid, polar lipid, mycolic acid, quinone, and fatty acid, which presented some high homogeneity. Most of the tested samples contained mycolic acid; glucose and arabinose as the main sugars; aspartic acid, arginine, lysine, tyrosine, and alanine as the main amino acids; and C16:0, C18:0, C18:2cis-9,12, anteiso-C14:0, and summed feature 8 as the main fatty acids. In addition, their polar lipid profiles were investigated for the first time, which significantly varied among Pleurotus species. The genus Pleurotus contained menaquinone-6 as the sole respiratory quinone, which showed a significant difference with that of its closely related genera. These results of this study demonstrated that the combined method above could efficiently differentiate each Pleurotus species and thus be considered an efficient tool for surveying the genetic diversity of the genus Pleurotus.

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Section: Resultsmentioning

confidence: 99%

Genetic and Chemical Diversity of Edible Mushroom Pleurotus Species

Lin

Yan

Kook

et al. 2022

BioMed Research International

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“…A similar region of rDNA was also used to study the phylogenetic relationships in Agricus, 21 Amanita, 22 Coprinus, 23 Ganoderma, 24 and Pleurotus. 11 Iracabal et al 10 generated UPGMA and NJ trees for the Pleurotus species based on LSU rDNA RFLP data. They found only one major difference between the two trees: P. ostreatus, P. columbinus, and P. cornucopiae isolates were separated into three clusters on the UPGMA tree, but were joined into a common larger cluster on the NJ tree.…”

Section: Discussionmentioning

confidence: 99%

“…5 In recent decades, biochemical and molecular criteria, including isoeletric focusing analysis and isozyme electrophoresis, have been used to determine the intraspecific and interspecific relationships among Pleurotus species. 6,7 Molecular analyses based on restriction fragment length polymorphism (RFLP) of total DNA, 8 mitochondrial DNA, 9 and ribosomal DNA, 10,11 sequence and structure analysis of mitochondrial rRNA, 12 and sequence analysis of ribosomal DNA 3,13 have also been useful for understanding phylogenetic relationships as well as the taxonomical identification of Pleurotus species.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of oyster mushrooms (Pleurotus spp.) based on restriction fragment length polymorphisms of the 5′ portion of 26S rDNA

et al. 2004

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Polymorphism analysis of the 5Ј portion of 26S rDNA from 34 Pleurotus strains (12 intersterility groups) collected mainly from Asia was performed. By combining the restriction fragment length polymorphism (RFLP) patterns obtained from digestions with seven restriction enzymes, the 34 Pleurotus strains were assigned to 11 RFLP types. Ten RFLP patterns corresponded with biological species, but one pattern was found in intersterility groups I (P. ostreatus complex), II (P. pulmonarius complex), and VIII (P. eryngii). The phylogenetic tree suggests that Pleurotus species have evolved in two patterns based on 26S rDNA RFLP data. One major cluster comprising the "P. ostreatus clade" is separated by relatively short branches, suggesting that the P. ostreatus complex, the P. pulmonarius complex, P. eryngii, and P. nebrodensis (intersterility groups I, II, VIII, and IX, respectively), share a recent common ancestor. RFLP data did not distinguish the species in the intersterility groups I, II, and VIII. The other major cluster apparently divided into five sublevel clusters in early stages of evolution and these clades split into terminal nodes in late stages of evolution: the P. calyptratussalmoneostramineus clade, the P. cornucopiae-ulmarius clade, the P. dryinus clade, the P. corticatus clade, and the P. cystidiosus-smithii clade.

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“…can undergo a drastic loss of diversity resulting from man’s selection [11, 14]. This genetic erosion increases genetic vulnerability and it may lead to dramatic effects on the production yield during cultivation [8].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism

et al. 2012

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Pleurotus strains are the most important fungi used in the agricultural industry. The exact characterization and identification of Pleurotus species is fundamental for correct identification of the individuals and exploiting their full potential in food industry. The amplified fragment length polymorphism (AFLP) method was applied for genomic fingerprinting of 21 Pleurotus isolates of Asian and European origin. Using one Pst I restriction endonuclease and four selective primers in an AFLP assay, 371 DNA fragments were generated, including 308 polymorphic bands. The AFLP profiles were found to be highly specific for each strain and they unambiguously distinguished 21 Pleurotus sp. fungi. The coefficient of Jaccard’s genome profile similarity between the analyzed strains ranged from 0.0 ( Pleurotus sp. I vs. P. sajor - caju 237 and P. eryngii 238) to 0.750 ( P. ostreatus 246 vs. P. ostreatus 248), and the average was 0.378. The AFLP-based dendrogram generated by the UPGMA method grouped all the Pleurotus fungi studied into two major clusters and one independent lineage located on the outskirt of the tree occupied by naturally growing Pleurotus species strain I. The results of the present study suggest the possible applicability of the AFLP- Pst I method in effective identification and molecular characterization of Pleurotus sp. strains.

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Molecular systematics of the genus Pleurotus: analysis of restriction polymorphisms in ribosomal DNA

Cited by 30 publications

References 41 publications

Genetic and Chemical Diversity of Edible Mushroom Pleurotus Species

Genetic and Chemical Diversity of Edible Mushroom Pleurotus Species

Phylogenetic analysis of oyster mushrooms (Pleurotus spp.) based on restriction fragment length polymorphisms of the 5′ portion of 26S rDNA

Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism

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